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FR2793807B1 - Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre - Google Patents

Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre

Info

Publication number
FR2793807B1
FR2793807B1 FR9906419A FR9906419A FR2793807B1 FR 2793807 B1 FR2793807 B1 FR 2793807B1 FR 9906419 A FR9906419 A FR 9906419A FR 9906419 A FR9906419 A FR 9906419A FR 2793807 B1 FR2793807 B1 FR 2793807B1
Authority
FR
France
Prior art keywords
kits
fragment
vector
applications
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9906419A
Other languages
English (en)
Other versions
FR2793807A1 (fr
Inventor
Marc Delcourt
Stephane Blesa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomethodes SA
Original Assignee
Biomethodes SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes SA filed Critical Biomethodes SA
Priority to FR9906419A priority Critical patent/FR2793807B1/fr
Priority to PCT/FR2000/001349 priority patent/WO2000071730A1/fr
Priority to AU47649/00A priority patent/AU4764900A/en
Publication of FR2793807A1 publication Critical patent/FR2793807A1/fr
Application granted granted Critical
Publication of FR2793807B1 publication Critical patent/FR2793807B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR9906419A 1999-05-20 1999-05-20 Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre Expired - Fee Related FR2793807B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR9906419A FR2793807B1 (fr) 1999-05-20 1999-05-20 Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre
PCT/FR2000/001349 WO2000071730A1 (fr) 1999-05-20 2000-05-18 Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre
AU47649/00A AU4764900A (en) 1999-05-20 2000-05-18 Method for inserting a nucleic acid fragment in a vector, uses thereof and kits therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9906419A FR2793807B1 (fr) 1999-05-20 1999-05-20 Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre

Publications (2)

Publication Number Publication Date
FR2793807A1 FR2793807A1 (fr) 2000-11-24
FR2793807B1 true FR2793807B1 (fr) 2003-05-16

Family

ID=9545810

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9906419A Expired - Fee Related FR2793807B1 (fr) 1999-05-20 1999-05-20 Methode d'insertion d'un fragment d'acide nucleique dans un vecteur, ses applications et les kits pour sa mise en oeuvre

Country Status (3)

Country Link
AU (1) AU4764900A (fr)
FR (1) FR2793807B1 (fr)
WO (1) WO2000071730A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US20020146732A1 (en) 2001-02-02 2002-10-10 Padgett Hal S. Method of increasing complementarity in a heteroduplex
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
CN118546962A (zh) * 2024-05-17 2024-08-27 广州派真生物技术有限公司 一种基于引物连接的载体构建试剂盒及方法和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8926269D0 (en) * 1989-11-21 1990-01-10 Dynal As Plasmid
US5523221A (en) * 1993-06-16 1996-06-04 Stratagene Method for the directional cloning of DNA
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method

Also Published As

Publication number Publication date
AU4764900A (en) 2000-12-12
FR2793807A1 (fr) 2000-11-24
WO2000071730A1 (fr) 2000-11-30

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Legal Events

Date Code Title Description
ST Notification of lapse

Effective date: 20080131