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FR2788281B1 - Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus - Google Patents

Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus

Info

Publication number
FR2788281B1
FR2788281B1 FR9816006A FR9816006A FR2788281B1 FR 2788281 B1 FR2788281 B1 FR 2788281B1 FR 9816006 A FR9816006 A FR 9816006A FR 9816006 A FR9816006 A FR 9816006A FR 2788281 B1 FR2788281 B1 FR 2788281B1
Authority
FR
France
Prior art keywords
adipocytes
producing
undifferentiated fibroblasts
undifferentiated
fibroblasts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9816006A
Other languages
English (en)
Other versions
FR2788281A1 (fr
Inventor
Jerome Gros
Cinderella Gerhardt
Arthur Donny Strosberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR9816006A priority Critical patent/FR2788281B1/fr
Priority to PCT/FR1999/003174 priority patent/WO2000037607A2/fr
Priority to AU16647/00A priority patent/AU1664700A/en
Publication of FR2788281A1 publication Critical patent/FR2788281A1/fr
Application granted granted Critical
Publication of FR2788281B1 publication Critical patent/FR2788281B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/81—Adrenaline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR9816006A 1998-12-18 1998-12-18 Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus Expired - Fee Related FR2788281B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR9816006A FR2788281B1 (fr) 1998-12-18 1998-12-18 Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus
PCT/FR1999/003174 WO2000037607A2 (fr) 1998-12-18 1999-12-17 Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus
AU16647/00A AU1664700A (en) 1998-12-18 1999-12-17 Method for producing adipocytes from non-differentiated fibroblasts and use of resulting adipocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9816006A FR2788281B1 (fr) 1998-12-18 1998-12-18 Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus

Publications (2)

Publication Number Publication Date
FR2788281A1 FR2788281A1 (fr) 2000-07-13
FR2788281B1 true FR2788281B1 (fr) 2001-03-09

Family

ID=9534136

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9816006A Expired - Fee Related FR2788281B1 (fr) 1998-12-18 1998-12-18 Methode de production d'adipocytes a partir de fibroblastes non differencies ainsi que l'utilisation des adipocytes ainsi obtenus

Country Status (3)

Country Link
AU (1) AU1664700A (fr)
FR (1) FR2788281B1 (fr)
WO (1) WO2000037607A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153432A (en) * 1999-01-29 2000-11-28 Zen-Bio, Inc Methods for the differentiation of human preadipocytes into adipocytes
DE502004004521D1 (de) * 2004-10-01 2007-09-13 Curacyte Discovery Gmbh Verwendung von differenzierten Fibroblasten oder einer primären Fibroblastkultur zur Transdifferenzierung in Fett-, Knochen- und Knorpelzellen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789654A (en) * 1996-05-09 1998-08-04 Beth Israel Hospital Association Transgenic animals deficient in endogenous β3 -adrenergic receptor and uses thereof

Also Published As

Publication number Publication date
WO2000037607A2 (fr) 2000-06-29
WO2000037607A3 (fr) 2000-11-09
FR2788281A1 (fr) 2000-07-13
AU1664700A (en) 2000-07-12

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Legal Events

Date Code Title Description
ST Notification of lapse