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FI971129A7 - Hydrofobisten amplifiointituotteiden detektointi uuttamalla orgaaniseen faasiin - Google Patents

Hydrofobisten amplifiointituotteiden detektointi uuttamalla orgaaniseen faasiin Download PDF

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Publication number
FI971129A7
FI971129A7 FI971129A FI971129A FI971129A7 FI 971129 A7 FI971129 A7 FI 971129A7 FI 971129 A FI971129 A FI 971129A FI 971129 A FI971129 A FI 971129A FI 971129 A7 FI971129 A7 FI 971129A7
Authority
FI
Finland
Prior art keywords
hydrophobic
extraction
detection
organic phase
amplification products
Prior art date
Application number
FI971129A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI971129A0 (fi
Inventor
G Terrance Walker
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of FI971129A0 publication Critical patent/FI971129A0/fi
Publication of FI971129A7 publication Critical patent/FI971129A7/fi

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
FI971129A 1995-07-19 1997-03-18 Hydrofobisten amplifiointituotteiden detektointi uuttamalla orgaaniseen faasiin FI971129A7 (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/503,964 US5550025A (en) 1995-07-19 1995-07-19 Detection of hydrophobic amplification products by extraction into an organic phase
PCT/US1996/011977 WO1997004132A1 (en) 1995-07-19 1996-07-18 Detection of hydrophobic amplification products by extraction into an organic phase

Publications (2)

Publication Number Publication Date
FI971129A0 FI971129A0 (fi) 1997-03-18
FI971129A7 true FI971129A7 (fi) 1997-05-16

Family

ID=24004274

Family Applications (1)

Application Number Title Priority Date Filing Date
FI971129A FI971129A7 (fi) 1995-07-19 1997-03-18 Hydrofobisten amplifiointituotteiden detektointi uuttamalla orgaaniseen faasiin

Country Status (10)

Country Link
US (1) US5550025A (es)
EP (1) EP0783588A1 (es)
JP (1) JP3061419B2 (es)
KR (1) KR100226297B1 (es)
AU (1) AU712978B2 (es)
BR (1) BR9606520A (es)
CA (1) CA2200347C (es)
FI (1) FI971129A7 (es)
MX (1) MX9702000A (es)
WO (1) WO1997004132A1 (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5631147A (en) * 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
US5667994A (en) * 1996-02-28 1997-09-16 Becton, Dickinson And Company Amplification and detection of mycobacterium avium complex species
CA2249717A1 (en) * 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
US5719031A (en) * 1996-08-14 1998-02-17 Molecular Probes, Inc. Dye labeled polymers as reagents for measuring polymer degradation
US6204002B1 (en) * 1996-10-22 2001-03-20 Daikin Industries, Ltd. Gangliosides having fluorescent-tagged ceramide moieties
US6287822B1 (en) 1997-08-05 2001-09-11 Transgenomic, Inc. Mutation detection method
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5863736A (en) * 1997-05-23 1999-01-26 Becton, Dickinson And Company Method, apparatus and computer program products for determining quantities of nucleic acid sequences in samples
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
EP1023463A4 (en) 1997-10-09 2003-01-02 Transgenomic Inc MODIFICATION OF DOUBLE-STRANDED DNA TO IMPROVE CHROMATOGRAPHIC SEPARATIONS OF MATCHED ION POLYNUCLEOTIDES
US6060324A (en) * 1997-11-12 2000-05-09 Phytochem Technologies, Inc. Fluorometric assay composition for measurement of antioxidant activity
US6066458A (en) * 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
US6455692B1 (en) 1998-08-04 2002-09-24 Transgenomic, Inc. Method of concentrating polynucleotides using MIPC
US6238868B1 (en) * 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US20020025519A1 (en) * 1999-06-17 2002-02-28 David J. Wright Methods and oligonucleotides for detecting nucleic acid sequence variations
US20030165913A1 (en) 1999-06-17 2003-09-04 Sha-Sha Wang Methods for detecting nucleic acid sequence variations
US6316200B1 (en) 2000-06-08 2001-11-13 Becton, Dickinson And Company Probes and methods for detection of nucleic acids
US6379888B1 (en) 1999-09-27 2002-04-30 Becton, Dickinson And Company Universal probes and methods for detection of nucleic acids
US6258546B1 (en) 2000-06-23 2001-07-10 Becton, Dickinson And Company Detection of nucleic acid amplification
KR20050008720A (ko) 2002-05-17 2005-01-21 벡톤 디킨슨 앤드 컴퍼니 표적 핵산 서열의 분리, 증폭 및 검출을 위한 자동화 시스템
US20040175737A1 (en) * 2002-12-23 2004-09-09 Wyeth Assay for RNase H activity
CN101824409B (zh) * 2003-04-25 2015-11-25 贝克顿·迪金森公司 通过核酸扩增检测1型和2型单纯疱疹病毒
US7521185B2 (en) * 2003-09-12 2009-04-21 Becton, Dickinson And Company Assay for SARS coronavirus by amplification and detection of the replicase sequence
CA2538086A1 (en) * 2003-09-12 2005-03-24 Becton, Dickinson And Company Assay for sars coronavirus by amplification and detection of nucleocapsid rna sequence
US20050233332A1 (en) * 2004-04-14 2005-10-20 Collis Matthew P Multiple fluorophore detector system
US20060073511A1 (en) 2004-10-05 2006-04-06 Affymetrix, Inc. Methods for amplifying and analyzing nucleic acids

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
JP3030417B2 (ja) * 1989-03-03 2000-04-10 ジェネンテク,インコーポレイテッド インビトロでのdnaの増幅、ゲノムクローニングおよびマッピングの、改良された方法
US5348853A (en) * 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
CA2145719C (en) * 1994-04-18 1998-06-30 James G. Nadeau Detection of nucleic acid amplification
AU705637B2 (en) * 1994-09-23 1999-05-27 Becton Dickinson & Company Detection of nucleic acid amplification

Also Published As

Publication number Publication date
EP0783588A1 (en) 1997-07-16
FI971129A0 (fi) 1997-03-18
AU712978B2 (en) 1999-11-18
US5550025A (en) 1996-08-27
KR100226297B1 (ko) 1999-10-15
AU6648596A (en) 1997-02-18
JP3061419B2 (ja) 2000-07-10
JPH10502259A (ja) 1998-03-03
WO1997004132A1 (en) 1997-02-06
BR9606520A (pt) 1997-12-23
CA2200347C (en) 2000-12-05
MX9702000A (es) 1997-06-28
CA2200347A1 (en) 1997-02-06
KR970706404A (ko) 1997-11-03

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