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FI871919A7 - Erityssignaaliselektiovektoreita solunulkoiseen proteiinisynteesiin Bacillus-bakteereilla - Google Patents

Erityssignaaliselektiovektoreita solunulkoiseen proteiinisynteesiin Bacillus-bakteereilla Download PDF

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Publication number
FI871919A7
FI871919A7 FI871919A FI871919A FI871919A7 FI 871919 A7 FI871919 A7 FI 871919A7 FI 871919 A FI871919 A FI 871919A FI 871919 A FI871919 A FI 871919A FI 871919 A7 FI871919 A7 FI 871919A7
Authority
FI
Finland
Prior art keywords
protein synthesis
signal selection
secretion signal
extracellular protein
bacillus bacteria
Prior art date
Application number
FI871919A
Other languages
English (en)
Swedish (sv)
Other versions
FI871919A0 (fi
FI871919L (fi
Inventor
Ee Jan Hendrik Van
Ben P H Peeters
Gerard Venema
Hilde E Smith
Sierd Bron
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of FI871919A0 publication Critical patent/FI871919A0/fi
Publication of FI871919L publication Critical patent/FI871919L/fi
Publication of FI871919A7 publication Critical patent/FI871919A7/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
FI871919A 1986-05-02 1987-04-29 Erityssignaaliselektiovektoreita solunulkoiseen proteiinisynteesiin Bacillus-bakteereilla FI871919A7 (fi)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP86200774 1986-05-02

Publications (3)

Publication Number Publication Date
FI871919A0 FI871919A0 (fi) 1987-04-29
FI871919L FI871919L (fi) 1987-11-03
FI871919A7 true FI871919A7 (fi) 1987-11-03

Family

ID=8195736

Family Applications (1)

Application Number Title Priority Date Filing Date
FI871919A FI871919A7 (fi) 1986-05-02 1987-04-29 Erityssignaaliselektiovektoreita solunulkoiseen proteiinisynteesiin Bacillus-bakteereilla

Country Status (10)

Country Link
US (1) US5037760A (fi)
EP (1) EP0244042B1 (fi)
JP (1) JPS6339586A (fi)
AT (1) ATE110111T1 (fi)
DE (1) DE3750378T2 (fi)
DK (1) DK221087A (fi)
ES (1) ES2061482T3 (fi)
FI (1) FI871919A7 (fi)
IE (1) IE64916B1 (fi)
PT (1) PT84801B (fi)

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US5304637A (en) * 1987-07-13 1994-04-19 Gist-Brocades N.V. Expression and purification of human interleukin-3 and muteins thereof
US6384194B1 (en) 1987-12-16 2002-05-07 Dsm N.V. Expression and purification of human interleukin-3 and muteins thereof
US5242821A (en) * 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria
ES2109336T3 (es) 1990-11-26 1998-01-16 Genetics Inst Expresion de pace en celulas huesped y metodos de utilizacion de la misma.
US5422427A (en) * 1991-09-17 1995-06-06 The United States Of America As Represented By The United States Department Of Health And Human Services Pneumococcal fimbrial protein A
US6312944B1 (en) 1991-11-14 2001-11-06 The United States Of America As Represented By The Department Of Health And Human Services Pneumococcal fimbrial protein A
US5854416A (en) * 1991-11-14 1998-12-29 The United States Of America As Represented By The Department Of Health And Human Services Streptococcus pneumoniae 37-KDA surface adhesin a protein and nucleic acids coding therefor
GB9513371D0 (en) 1995-06-30 1995-09-06 Biocine Spa Immunogenic detoxified mutant toxins
JP2879303B2 (ja) * 1993-01-14 1999-04-05 佑 本庶 ｃＤＮＡライブラリーの作製方法、および新規なポリペプチドとそれをコードするＤＮＡ
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
US5789653A (en) * 1995-01-10 1998-08-04 University Of Edinburgh Secretory gene trap
EP0832209A1 (en) * 1995-06-07 1998-04-01 ZymoGenetics, Inc. Secretion leader trap cloning method
US5753462A (en) * 1995-06-07 1998-05-19 Zymogenetics, Inc. Secretion leader trap cloning method
FR2739629B1 (fr) * 1995-10-06 1997-12-26 Systems Bio Ind Utilisation d'un systeme de secretion sec-dependant pour secreter des proteines normalement secretees par un systeme de secretion sec-independant, bacteries les contenant et leur utilisation
CA2252425A1 (en) * 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
US6521440B1 (en) 1997-09-15 2003-02-18 Genencor International, Inc. Proteases from gram-positive organisms
US6300117B1 (en) * 1997-09-15 2001-10-09 Genencor International, Inc. Proteases from gram-positive organisms
GB9719637D0 (en) * 1997-09-15 1997-11-19 Genencor Int Bv Proteases from gram-positive organisms
AU9363798A (en) 1997-11-06 1999-05-31 Chiron S.P.A. Neisserial antigens
CA2317815A1 (en) 1998-01-14 1999-07-22 Chiron S.P.A. Neisseria meningitidis antigens
US6103472A (en) * 1998-02-20 2000-08-15 Amgen Inc. Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
AU758764B2 (en) * 1998-03-02 2003-03-27 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Epitope peptides immunogenic against (streptococcus pneumoniae)
US6228590B1 (en) 1998-03-23 2001-05-08 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
WO2001031019A2 (en) 1999-10-29 2001-05-03 Chiron Spa Neisserial antigenic peptides
PT1944371E (pt) 1998-05-01 2015-07-13 Novartis Ag Antigénios e composições da neisseria meningitidis
EP2290083B1 (en) 1999-04-30 2014-08-20 Novartis Vaccines and Diagnostics S.r.l. Conserved neisserial antigens
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
GB9916529D0 (en) 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
JP2003520248A (ja) 2000-01-17 2003-07-02 カイロン エセ．ピー．アー． Ｎ．ｍｅｎｉｎｇｉｔｉｄｉｓ血清型ｂ外膜タンパク質を含む外膜小胞（ｏｍｖ）ワクチン
WO2001064863A2 (en) * 2000-02-28 2001-09-07 Stratagene A screening system to identify polynucleotides encoding cleavable n-terminal signal sequences
NZ594877A (en) 2000-10-27 2012-07-27 Novartis Vaccines & Diagnostic Nucleic acids and proteins from streptococcus groups A & B
GB0107661D0 (en) 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
GB0107658D0 (en) 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
EP1417347A4 (en) * 2001-05-02 2005-06-22 Univ South Florida VECTOR SYSTEM FOR THE SELECTION OF SEPARATED PROTEINS AND MEMBRANE-BONDED PROTEINS CODING GENES
EP2335723A1 (en) 2001-12-12 2011-06-22 Novartis Vaccines and Diagnostics S.r.l. Immunisation against chlamydia trachomatis
US20070059329A1 (en) 2002-11-15 2007-03-15 Nathalie Norais Unexpected surface proteins in meningococcus
GB0308198D0 (en) 2003-04-09 2003-05-14 Chiron Srl ADP-ribosylating bacterial toxin
DK1713930T3 (da) 2004-01-17 2011-01-17 Korea Res Inst Of Bioscience Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
US20100015168A1 (en) 2006-06-09 2010-01-21 Novartis Ag Immunogenic compositions for streptococcus agalactiae
JP2009017840A (ja) * 2007-07-13 2009-01-29 Japan Agengy For Marine-Earth Science & Technology 外来遺伝子を細胞に安定に保持する方法
EP2573122A3 (en) * 2008-12-04 2013-07-10 Korea Research Institute of Bioscience and Biotechnology Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins
KR101291241B1 (ko) 2010-11-04 2013-08-01 한국생명공학연구원 효모에서 인체 상피세포 성장인자를 대량 생산하는 방법

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US4711843A (en) * 1980-12-31 1987-12-08 Cetus Corporation Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis
US4585739A (en) * 1983-03-07 1986-04-29 E. I. Du Pont De Nemours And Company Plasmid for foreign gene expression in B. subtilis
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
JPS60126087A (ja) * 1983-12-12 1985-07-05 Agency Of Ind Science & Technol プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６
NL8400687A (nl) * 1984-03-02 1985-10-01 Unilever Nv Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.
AU5668486A (en) * 1985-03-29 1986-10-23 Biotechnica International Inc. Secretion vector
JPS61271988A (ja) * 1985-05-27 1986-12-02 Agency Of Ind Science & Technol プラスミド

Also Published As

Publication number Publication date
DK221087A (da) 1987-11-03
PT84801A (en) 1987-05-01
ES2061482T3 (es) 1994-12-16
PT84801B (pt) 1989-12-29
EP0244042A1 (en) 1987-11-04
ATE110111T1 (de) 1994-09-15
IE871156L (en) 1987-11-02
FI871919A0 (fi) 1987-04-29
FI871919L (fi) 1987-11-03
DK221087D0 (da) 1987-04-30
US5037760A (en) 1991-08-06
DE3750378T2 (de) 1995-01-19
EP0244042B1 (en) 1994-08-17
DE3750378D1 (de) 1994-09-22
IE64916B1 (en) 1995-09-20
JPS6339586A (ja) 1988-02-20

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MM Patent lapsed

Owner name: GIST-BROCADES N.V.