FI871288A0 - Förfarande för framställning av aprotinin med rekombinant-DNA-teknik och vid förfarandet användbart DNA, expressionsplasmid och värdcell - Google Patents

Förfarande för framställning av aprotinin med rekombinant-DNA-teknik och vid förfarandet användbart DNA, expressionsplasmid och värdcell

Info

Publication number: FI871288A0
Authority: FI; Finland
Prior art keywords: aprotinin; preparation; host cell; expression plasmid; method useful
Prior art date: 1986-03-26

Application number

FI871288A

Other languages

English (en)

Finnish (fi)

Other versions

FI94876B (sv

FI871288L (sv

FI94876C (sv

Inventor

Ernst-August Auerswald

Werner Schroeder

Eugen Schnabel

Wolfgang Bruns

Gerd Reinhardt

Michael Kotick

Original Assignee

Bayer Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-03-26

Filing date

1987-03-24

Publication date

1987-03-24

1987-03-24 Application filed by Bayer Ag filed Critical Bayer Ag

1987-03-24 Publication of FI871288A0 publication Critical patent/FI871288A0/sv

1987-09-27 Publication of FI871288L publication Critical patent/FI871288L/sv

1995-07-31 Publication of FI94876B publication Critical patent/FI94876B/sv

1995-11-10 Application granted granted Critical

1995-11-10 Publication of FI94876C publication Critical patent/FI94876C/sv

Links

108010039627 Aprotinin Proteins 0.000 title abstract 3
229960004405 aprotinin Drugs 0.000 title abstract 3
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 title abstract 3
238000000034 method Methods 0.000 title 2
108020004414 DNA Proteins 0.000 title 1
108020004511 Recombinant DNA Proteins 0.000 title 1
239000013613 expression plasmid Substances 0.000 title 1
102000016387 Pancreatic elastase Human genes 0.000 abstract 2
108010067372 Pancreatic elastase Proteins 0.000 abstract 2
108010028275 Leukocyte Elastase Proteins 0.000 abstract 1
102000016799 Leukocyte elastase Human genes 0.000 abstract 1
210000002966 serum Anatomy 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/14—Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Medicinal Chemistry (AREA)
Pharmacology & Pharmacy (AREA)
Veterinary Medicine (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Chemical Kinetics & Catalysis (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
General Chemical & Material Sciences (AREA)
Cell Biology (AREA)
Crystallography & Structural Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Tropical Medicine & Parasitology (AREA)
Vascular Medicine (AREA)
Heart & Thoracic Surgery (AREA)
Rheumatology (AREA)
Cardiology (AREA)
Virology (AREA)
Pain & Pain Management (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

FI871288A 1986-03-26 1987-03-24 Förfarande för framställning av aprotinin med rekombinant-DNA-teknik och vid förfarandet användbart DNA, expressionsplasmid och värdcell FI94876C (sv)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB08607523A GB2188322A (en)	1986-03-26	1986-03-26	Aprotinin and analogues thereof produced by a recombinant host
GB8607523		1986-03-26

Publications (4)

Publication Number	Publication Date
FI871288A0 true FI871288A0 (sv)	1987-03-24
FI871288L FI871288L (sv)	1987-09-27
FI94876B FI94876B (sv)	1995-07-31
FI94876C FI94876C (sv)	1995-11-10

Family

ID=10595278

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI871288A FI94876C (sv)	1986-03-26	1987-03-24	Förfarande för framställning av aprotinin med rekombinant-DNA-teknik och vid förfarandet användbart DNA, expressionsplasmid och värdcell

Country Status (15)

Country	Link
US (1)	US4894436A (sv)
EP (1)	EP0238993B1 (sv)
JP (1)	JP2537507B2 (sv)
KR (2)	KR950000302B1 (sv)
CN (1)	CN1018793B (sv)
AT (1)	ATE123301T1 (sv)
AU (1)	AU600475B2 (sv)
CA (1)	CA1338309C (sv)
DE (1)	DE3751324D1 (sv)
DK (1)	DK151687A (sv)
ES (1)	ES2073390T3 (sv)
FI (1)	FI94876C (sv)
GB (1)	GB2188322A (sv)
IL (1)	IL81964A (sv)
ZA (1)	ZA872181B (sv)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB2188933A (en) *	1986-04-10	1987-10-14	Bayer Ag	Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby
US5654176A (en) *	1987-05-28	1997-08-05	Amrad Corporation Limited	Fusion proteins containing glutathione-s-transferase
DE3724570A1 (de) *	1987-06-27	1989-01-05	Bayer Ag	Human-aprotinin, dessen lys-rest in position 15 gegen einen anderen protogenen aminosaeurerest ausgetauscht ist
US5591603A (en) *	1987-08-28	1997-01-07	Novo Nordisk A/S	Process for preparing aprotinin and aprotinin analogs in yeast cells
DK225488D0 (da) *	1988-04-26	1988-04-26	Novo Industri As	Polypeptid
ES2063161T3 (es) *	1988-03-07	1995-01-01	Ciba Geigy Ag	Proteinas modificadas.
US5180667A (en) *	1988-03-07	1993-01-19	Ciba-Geigy Corporation	Genes encoding eglin C mutants
US20060134087A1 (en) *	1988-09-02	2006-06-22	Dyax Corp.	ITI-D1 Kunitz domain mutants as hNE inhibitors
US5223409A (en)	1988-09-02	1993-06-29	Protein Engineering Corp.	Directed evolution of novel binding proteins
US7413537B2 (en)	1989-09-01	2008-08-19	Dyax Corp.	Directed evolution of disulfide-bonded micro-proteins
DE3930522A1 (de) *	1989-09-13	1991-03-21	Bayer Ag	Rekombinante aprotinin-varianten - gentechnisches verfahren zur mikrobiellen herstellung von homogen prozessierten aprotinin-varianten sowie die therapeutische anwendung derselben
US5231010A (en) *	1989-09-13	1993-07-27	Bayer Aktiengesellschaft	Recombinant aprotinin variants genetically engineered process for the microbial preparation of homogeneously processed aprotinin variants and the therapeutic use thereof
AU8740491A (en) *	1990-09-28	1992-04-28	Protein Engineering Corporation	Proteinaceous anti-dental plaque agents
IL99585A0 (en) *	1990-10-01	1992-08-18	Novo Nordisk As	Aprotinin analogues,their production and pharmaceutical compositions containing them
EP0486001A1 (en) *	1990-11-13	1992-05-20	Mochida Pharmaceutical Co., Ltd.	Recombinant urinary trypsin inhibitor fragments and drug composition
DK1279731T3 (da)	1991-03-01	2007-09-24	Dyax Corp	Fremgangsmåde til udvikling af bindende miniproteiner
DE69230061T2 (de) *	1991-07-25	2000-01-05	Oriental Yeast Co. Ltd., Tokio/Tokyo	Künstliches immunoglobulin-bindendes protein
US5795954A (en) *	1994-03-04	1998-08-18	Genentech, Inc.	Factor VIIa inhibitors from Kunitz domain proteins
US5780265A (en) *	1995-06-05	1998-07-14	Genentech, Inc.	Kunitz type plasma kallikrein inhibitors
US5786328A (en) *	1995-06-05	1998-07-28	Genentech, Inc.	Use of kunitz type plasma kallikrein inhibitors
US5824870A (en) *	1995-11-06	1998-10-20	Baszczynski; Chris	Commercial production of aprotinin in plants
WO1998024474A1 (en) *	1996-12-06	1998-06-11	Fonden Til Fremme Af Eksperimentel Cancerforskning	Inhibition of invasive remodelling
FR2774988B1 (fr) *	1998-02-16	2000-05-05	Commissariat Energie Atomique	Procede de purification de la prpres a partir d'un echantillon biologique et ses applications
US7241730B2 (en) *	1998-08-27	2007-07-10	Universitat Zurich	Enzyme-mediated modification of fibrin for tissue engineering: fibrin formulations with peptides
US7910550B2 (en) *	2004-04-16	2011-03-22	Stc.Unm	Human kunitz-type inhibitor with enhanced antifibrinolytic activity
US7432238B2 (en) *	2004-04-16	2008-10-07	Stc.Unm	Human Kunitz-type inhibitor with enhanced antifibrinolytic activity
US20060218667A1 (en) *	2004-10-12	2006-09-28	Vojdani Fakhrieh S	Plant-produced recombinant aprotinin and aprotinin variants
EP1859041B2 (en) *	2005-02-18	2014-11-19	Angiochem Inc.	Aprotinin polypeptides for transporting a compound across the blood-brain barrier
CN100406558C (zh) *	2005-06-14	2008-07-30	吉林圣元科技有限责任公司	制备重组抑酶肽的方法
DK1976985T3 (da)	2005-12-29	2013-10-21	Univ California	Fremgangsmåder og præparater relateret til mutant Kunitz-domæne I af TFPI-2
WO2008110301A1 (de) *	2007-03-13	2008-09-18	Bayer Schering Pharma Aktiengesellschaft	Aprotinin-varianten mit verbesserten eigenschaften
DE102007056231A1 (de)	2007-09-08	2009-03-12	Bayer Healthcare Ag	Herstellung und Verwendung von Varianten humander Kunitz-Typ Protease-Inhibitoren (hKTPI)
CN116925291A (zh) *	2015-07-31	2023-10-24	罗门哈斯公司	用于合成单峰丙烯酸珠粒的寡聚物晶种

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3309501A1 (de) *	1983-03-17	1984-09-20	Bayer Ag, 5090 Leverkusen	Plasmid-vektoren zur klonierung, identifizierung und expression von genen
AU560584B2 (en) *	1983-07-28	1987-04-09	Bayer Aktiengesellschaft	Homologues of aprotinin
DE3410437A1 (de) *	1984-03-22	1985-09-26	Bayer Ag, 5090 Leverkusen	Verfahren zur herstellung von proteinen
EP0168342B1 (de) *	1984-06-14	1991-07-03	Ciba-Geigy Ag	Verfahren zur Herstellung von Thrombin-Inhibitoren
DE3581328D1 (de) *	1984-06-19	1991-02-21	Transgene Sa	Derivate des menschlichen alpha-1-antitrypins und verfahren zu deren herstellung.
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
ATE81673T1 (de) *	1984-08-16	1992-11-15	Bio Technology General Corp	Verfahren zur beseitigung von nendaminos\|ureresten aus eukaryotpolypeptidanlagen und so erzeugte polypeptide.
DE3523701A1 (de) *	1985-07-03	1987-01-08	Bayer Ag	Verfahren zur herstellung von proteinen und polypeptiden
GB2188933A (en) *	1986-04-10	1987-10-14	Bayer Ag	Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby

1986
- 1986-03-26 GB GB08607523A patent/GB2188322A/en not_active Withdrawn
1987
- 1987-03-11 AU AU69901/87A patent/AU600475B2/en not_active Ceased
- 1987-03-18 ES ES87103942T patent/ES2073390T3/es not_active Expired - Lifetime
- 1987-03-18 EP EP87103942A patent/EP0238993B1/de not_active Expired - Lifetime
- 1987-03-18 AT AT87103942T patent/ATE123301T1/de not_active IP Right Cessation
- 1987-03-18 DE DE3751324T patent/DE3751324D1/de not_active Expired - Fee Related
- 1987-03-23 IL IL81964A patent/IL81964A/xx not_active IP Right Cessation
- 1987-03-23 US US07/029,501 patent/US4894436A/en not_active Expired - Lifetime
- 1987-03-24 CA CA000532864A patent/CA1338309C/en not_active Expired - Fee Related
- 1987-03-24 FI FI871288A patent/FI94876C/sv not_active IP Right Cessation
- 1987-03-25 DK DK151687A patent/DK151687A/da not_active Application Discontinuation
- 1987-03-25 ZA ZA872181A patent/ZA872181B/xx unknown
- 1987-03-25 KR KR1019870002715A patent/KR950000302B1/ko not_active Expired - Fee Related
- 1987-03-26 JP JP62072938A patent/JP2537507B2/ja not_active Expired - Lifetime
- 1987-03-26 CN CN87102412A patent/CN1018793B/zh not_active Expired
- 1987-07-22 KR KR1019870007966A patent/KR960007194B1/ko not_active Expired - Fee Related

Also Published As

Publication number	Publication date
GB8607523D0 (en)	1986-04-30
DK151687A (da)	1987-09-27
KR890002401A (ko)	1989-04-10
EP0238993B1 (de)	1995-05-31
AU600475B2 (en)	1990-08-16
KR960007194B1 (ko)	1996-05-29
JP2537507B2 (ja)	1996-09-25
FI94876B (sv)	1995-07-31
US4894436A (en)	1990-01-16
FI871288L (sv)	1987-09-27
CA1338309C (en)	1996-05-07
IL81964A0 (en)	1987-10-20
ES2073390T3 (es)	1995-08-16
CN87102412A (zh)	1987-10-07
DK151687D0 (da)	1987-03-25
FI94876C (sv)	1995-11-10
IL81964A (en)	1993-07-08
EP0238993A2 (de)	1987-09-30
DE3751324D1 (de)	1995-07-06
KR950000302B1 (ko)	1995-01-13
CN1018793B (zh)	1992-10-28
KR870009025A (ko)	1987-10-22
ATE123301T1 (de)	1995-06-15
GB2188322A (en)	1987-09-30
AU6990187A (en)	1987-10-01
ZA872181B (en)	1987-12-30
JPS637794A (ja)	1988-01-13
EP0238993A3 (en)	1989-03-22

Publication	Publication Date	Title
FI871288A0 (sv)	1987-03-24	Förfarande för framställning av aprotinin med rekombinant-DNA-teknik och vid förfarandet användbart DNA, expressionsplasmid och värdcell
DK322188A (da)	1988-12-17	Fremgangsmaade til fremstilling af protein
BR9609270A (pt)	1999-05-11	Polinucleotídeo isolado e purificado peptídeo isolado e purificado processo para detectar a presença da câncer mamário em um paciente e kit para a detecção da presença de células neoplásticas mamárias em uma amostra
BR8301918A (pt)	1983-12-20	Proteina,sequencia de dna,veiculo de expressao replicavel,microorganismo ou cultura celular,plasmideo,composicao,cultura de celulas,utilizacao de tal proteina,processo de expressao,processo para producao de um veiculo de expressao e processo para preparacao de uma proteina uroquinase
TR199902124T2 (xx)	2000-06-21	�ltihapl� hastal�klar�n tedavisinde yararl� k��k molek�ller.
MY101204A (en)	1991-08-17	Solubilization of protein aggregates
FI830774A7 (sv)	1983-09-09	Expression, behandling och sekretion av ett heterologiskt protein med jäst.
DE3585953D1 (de)	1992-06-04	Dna-fragmente, expressionsvektoren, proteine, wirte und verfahren zur herstellung der proteine.
DK430486A (da)	1987-03-10	Fremgangsmaade til udvinding af protein
FI880017L (sv)	1988-07-08	Förfarande för framställning av varianter av pankreatisk sekretorisk trypsininhibitor och vid förfarandet använt DNA, använd vektor och värdcell
DE69031656D1 (de)	1997-12-04	Saccharinderivate zur verwendung als proteolytische enzyminhibitoren sowie verfahren zur herstellung
GR3007031T3 (sv)	1993-07-30
KR880701777A (ko)	1988-11-05	재조합 dna 방법에 의해 제조된 인간의 췌장 분비 트립신 억제제와 그의 제조방법
FI883005A7 (sv)	1988-12-25	Promotorer för expression av främmande DNA i metylotrofiska bakterier, deras tillvaratagande och deras användning.
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Date	Code	Title	Description
1995-07-03	BB	Publication of examined application
2004-08-31	MM	Patent lapsed	Owner name: BAYER AKTIENGESELLSCHAFT