ES8802075A1 - Procedimiento para la multiplicacion de un adn heterologo - Google Patents
Procedimiento para la multiplicacion de un adn heterologoInfo
- Publication number
- ES8802075A1 ES8802075A1 ES556975A ES556975A ES8802075A1 ES 8802075 A1 ES8802075 A1 ES 8802075A1 ES 556975 A ES556975 A ES 556975A ES 556975 A ES556975 A ES 556975A ES 8802075 A1 ES8802075 A1 ES 8802075A1
- Authority
- ES
- Spain
- Prior art keywords
- dna
- host
- multiplication
- procedure
- promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000013598 vector Substances 0.000 abstract 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Metal Extraction Processes (AREA)
- Manufacturing Cores, Coils, And Magnets (AREA)
- Production Of Multi-Layered Print Wiring Board (AREA)
Abstract
PROCEDIMIENTO PARA LA MULTIPLICACION DE UN ADN HETEROLOGO. COMPRENDE LAS OPERACIONES DE INSERTAR UN ADN HETEROLOGO, QUE CODIFICA UNA PROTEINA DESEADA, EN UN VECTOR DE LEVADURA DE COPIAS MULTIPLES, ESTABLE Y RECOMBINANTE; DE TRANSFORMAR EL VECTOR DENTRO DE UN ORGANISMO HOSPEDANTE DE LEVADURA APROPIADO QUE CONTIENE COPIAS DE <TIPO SILVESTRE> DE PLASMIDO CIRCULO 2/U; DE CULTIVAR EL HOSPEDANTE PRIMERAMENTE SOBRE UN MEDIOQUE DESACTIVA AL PROMOTOR REGULABLE; DE CULTIVAR EL HOSPEDANTE SOBRE UN MEDIO QUE REACTIVA AL PROMOTOR REGULABLE; DE TRANSFERIR EL HOSPEDANTE DE NUEVO AL MEDIO DESACTIVADOR DEL PROMOTOR REGULABLE; DE AISLAR Y PURIFICAR LOS VECTORES PROCEDENTES DE LAS CELULAS; Y DE AISLAR EL ADN HETEROLOGO A PARTIR DE LOS VECTORES Y DEPURIFICARLO A CONTINUACION.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843444495 DE3444495A1 (de) | 1984-12-04 | 1984-12-04 | Herstellung eines multi-kopien-hefe-vektors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8802075A1 true ES8802075A1 (es) | 1988-03-16 |
| ES556975A0 ES556975A0 (es) | 1988-03-16 |
Family
ID=6252051
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES549526A Expired ES8800342A1 (es) | 1984-12-04 | 1985-12-03 | Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinante |
| ES556975A Expired ES8802075A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la multiplicacion de un adn heterologo |
| ES556976A Expired ES8801705A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la expresion de una proteina heterologa |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES549526A Expired ES8800342A1 (es) | 1984-12-04 | 1985-12-03 | Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinante |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES556976A Expired ES8801705A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la expresion de una proteina heterologa |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP0184163A3 (es) |
| JP (1) | JPS61181385A (es) |
| AU (1) | AU5070685A (es) |
| DE (1) | DE3444495A1 (es) |
| DK (1) | DK559585A (es) |
| ES (3) | ES8800342A1 (es) |
| FI (1) | FI854781L (es) |
| GR (1) | GR852891B (es) |
| HU (1) | HU196454B (es) |
| NO (1) | NO854864L (es) |
| NZ (1) | NZ214422A (es) |
| PT (1) | PT81594B (es) |
| SU (1) | SU1364241A3 (es) |
| YU (1) | YU186985A (es) |
| ZA (1) | ZA859240B (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605035A1 (de) * | 1986-02-18 | 1987-08-20 | Strahlen Umweltforsch Gmbh | Plasmid pws101, verfahren zu seiner gewinnung und seine verwendung |
| DE4420785A1 (de) * | 1994-03-25 | 1995-10-05 | Basf Ag | Riboflavin-Biosynthese in Pilzen |
| EP3228707B1 (en) * | 2014-12-03 | 2020-09-23 | Kaneka Corporation | Vector containing centromere dna sequence and use thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL63035A (en) * | 1980-09-09 | 1985-05-31 | Univ California | Dnas capable of replication and stable mitotic maintenance in a host eukaryote,their preparation and eukaryotic cells comprising them |
-
1984
- 1984-12-04 DE DE19843444495 patent/DE3444495A1/de not_active Withdrawn
-
1985
- 1985-11-30 EP EP85115203A patent/EP0184163A3/de not_active Withdrawn
- 1985-12-02 YU YU01869/85A patent/YU186985A/xx unknown
- 1985-12-02 GR GR852891A patent/GR852891B/el unknown
- 1985-12-03 NO NO854864A patent/NO854864L/no unknown
- 1985-12-03 AU AU50706/85A patent/AU5070685A/en not_active Abandoned
- 1985-12-03 NZ NZ214422A patent/NZ214422A/en unknown
- 1985-12-03 HU HU854619A patent/HU196454B/hu not_active IP Right Cessation
- 1985-12-03 ES ES549526A patent/ES8800342A1/es not_active Expired
- 1985-12-03 FI FI854781A patent/FI854781L/fi not_active IP Right Cessation
- 1985-12-03 DK DK559585A patent/DK559585A/da not_active Application Discontinuation
- 1985-12-03 ZA ZA859240A patent/ZA859240B/xx unknown
- 1985-12-03 SU SU853991833A patent/SU1364241A3/ru active
- 1985-12-03 PT PT81594A patent/PT81594B/pt unknown
- 1985-12-04 JP JP60273181A patent/JPS61181385A/ja active Pending
-
1986
- 1986-07-23 ES ES556975A patent/ES8802075A1/es not_active Expired
- 1986-07-23 ES ES556976A patent/ES8801705A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES556976A0 (es) | 1988-02-16 |
| ZA859240B (en) | 1987-08-26 |
| NO854864L (no) | 1986-06-05 |
| EP0184163A2 (de) | 1986-06-11 |
| ES8800342A1 (es) | 1987-11-01 |
| HU196454B (en) | 1988-11-28 |
| FI854781A0 (fi) | 1985-12-03 |
| AU5070685A (en) | 1986-06-12 |
| ES8801705A1 (es) | 1988-02-16 |
| ES549526A0 (es) | 1987-11-01 |
| GR852891B (es) | 1986-04-03 |
| DK559585D0 (da) | 1985-12-03 |
| SU1364241A3 (ru) | 1987-12-30 |
| FI854781A7 (fi) | 1986-06-05 |
| DE3444495A1 (de) | 1986-06-05 |
| PT81594A (de) | 1986-01-01 |
| ES556975A0 (es) | 1988-03-16 |
| DK559585A (da) | 1986-06-05 |
| JPS61181385A (ja) | 1986-08-14 |
| YU186985A (en) | 1988-06-30 |
| HUT39775A (en) | 1986-10-29 |
| PT81594B (de) | 1987-10-16 |
| EP0184163A3 (de) | 1988-01-13 |
| NZ214422A (en) | 1988-02-12 |
| FI854781L (fi) | 1986-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL90423A (en) | Interleukin-1 inhibitors methods for producing them pharmaceutical compositions containing them isolated DNA sequences encoding interleukin-1 inhibitors and host cells and vectors containing them | |
| FI841977A7 (fi) | Stabiliserade plasmider | |
| ATE141517T1 (de) | Gen-sequenzentransfer und -exprimierung bei zellen des zentralen nervensystems mit mutierenden herpes-simplex-viren, die deletionen in viren-replikationsgenen enthalten | |
| DK577783A (da) | Vektor for udtrykkelse af et dna-segment i gaer | |
| EP0088195A3 (en) | Messenger rna, production and use thereof | |
| ATE143690T1 (de) | Cd40 kodierende dna | |
| AU7833494A (en) | Enhanced expression in plants using non-translated leader sequences | |
| AR008508A1 (es) | METODO PARA LOGRAR UNA EXPRESION ESPECíFICA MESoFILA DE UNA SECUENCIA DE NUCLEOTIDOS CODIFICANTE EN PLANTAS, CONTROLADA POR UN PROMOTOR , CASETES DE EXPRESION, VECTORES RECOMBINANTES Y MICROORGANISMOS QUE CONTIENEN DICHOS PROMOTORES, UTILIZACION DE DICHOS VECTORES Y PROMOTORES Y PROCEDIMIENTO PARA P | |
| ATE203269T1 (de) | Genherstellung zur produktion von transgenfisch | |
| GB8601597D0 (en) | Nucleotide sequences | |
| AU2812484A (en) | Dna vectors and there use in recombinant dna technology | |
| DK306890D0 (da) | Udtrykkelse af celleproliferation med decorin | |
| AU2353184A (en) | A polycistronic expression vector for vertebrate cells | |
| FI950628A0 (fi) | Modifioidut sienisolut ja menetelmä rekombinanttituotteiden tuottamiseksi | |
| DE59209981D1 (de) | Rekombinantes core-streptavidin | |
| DE69831417D1 (de) | Duale selektionkassette und sie enthaltende plasmide | |
| DK1021553T3 (da) | Eukaryotisk genekspressionskassette og anvendelser deraf | |
| DK0566554T3 (da) | Fremstilling af rekombinante proteiner ved anvendelse af herpesviruspromotorer og VP16-transaktivatorer | |
| ES8802075A1 (es) | Procedimiento para la multiplicacion de un adn heterologo | |
| NZ242124A (en) | Dna encoding an enzyme having arabinan-degrading activity | |
| AU6724794A (en) | GRB3-3 gene, its variants and their uses | |
| NZ241829A (en) | Oxalate oxidase, genes encoding it, vectors and transformed plants | |
| DK1097233T3 (da) | Promotor for vækstdifferentieringsfaktor og anvendelser deraf | |
| AU4641393A (en) | Use of mtr gene sequences for expression of foreign genes | |
| ATE256184T1 (de) | Promotor des utrophingens |