ES8704541A1 - Procedimiento para la preparacion de un vector con el sistema de expresion en trp - Google Patents
Procedimiento para la preparacion de un vector con el sistema de expresion en trpInfo
- Publication number
- ES8704541A1 ES8704541A1 ES554097A ES554097A ES8704541A1 ES 8704541 A1 ES8704541 A1 ES 8704541A1 ES 554097 A ES554097 A ES 554097A ES 554097 A ES554097 A ES 554097A ES 8704541 A1 ES8704541 A1 ES 8704541A1
- Authority
- ES
- Spain
- Prior art keywords
- sequence
- dna
- start codon
- ligation
- coli
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 3
- 108091081024 Start codon Proteins 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 230000004075 alteration Effects 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 5
- 238000003776 cleavage reaction Methods 0.000 abstract 4
- 230000007017 scission Effects 0.000 abstract 4
- 241000588724 Escherichia coli Species 0.000 abstract 3
- 108091033380 Coding strand Proteins 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000007515 enzymatic degradation Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- CJWXCNXHAIFFMH-AVZHFPDBSA-N n-[(2s,3r,4s,5s,6r)-2-[(2r,3r,4s,5r)-2-acetamido-4,5,6-trihydroxy-1-oxohexan-3-yl]oxy-3,5-dihydroxy-6-methyloxan-4-yl]acetamide Chemical compound C[C@H]1O[C@@H](O[C@@H]([C@@H](O)[C@H](O)CO)[C@@H](NC(C)=O)C=O)[C@H](O)[C@@H](NC(C)=O)[C@@H]1O CJWXCNXHAIFFMH-AVZHFPDBSA-N 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Complex Calculations (AREA)
- Advance Control (AREA)
- Electrotherapy Devices (AREA)
Abstract
PROCEDIMIENTO PARA PREPARAR UN VECTOR CON UN SISTEMA DE EXPRESION EN TRP. COMPRENDE: A) CORTAR CON LA ENZIMA DE RESTRICCION NCO I A UN VECTOR DE E. COLI QUE CONTIENE LA SECUENCIA DE ADN I (5KGTATCGACC) (HELICE CODIFICADORA) SEGUIDA POR 5K ATCG 3K; Y B) LIGAR AL VECTOR CORTADO UNA ESTRUCTURA DE GEN DE LA SECUENCIA ADN II (5K CATGX...3K) (3K Y...5K). SIENDO X E Y UN PAR COMPLEMENTARIO DE NUCLEOTIDOS DETRAS DEL CODON DE INICIACION DEL GEN ESTRUCTURAL. EL GEN ESTRUCTURAL EN EL EXTREMO 5K ES UN GEN PROPIO DEL HOSPEDANTE QUE CODIFICA UNA PROTEINA DE FUSION INSOLUBLE, UN INTERFERON O UNA INTERLEUCINA O SOMATOSTATINA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19853514113 DE3514113A1 (de) | 1985-04-19 | 1985-04-19 | Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8704541A1 true ES8704541A1 (es) | 1987-04-01 |
| ES554097A0 ES554097A0 (es) | 1987-04-01 |
Family
ID=6268543
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES554097A Expired ES8704541A1 (es) | 1985-04-19 | 1986-04-17 | Procedimiento para la preparacion de un vector con el sistema de expresion en trp |
| ES556233A Expired ES8706818A1 (es) | 1985-04-19 | 1986-06-19 | Procedimiento para preparar un polipeptido |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES556233A Expired ES8706818A1 (es) | 1985-04-19 | 1986-06-19 | Procedimiento para preparar un polipeptido |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP0198415B1 (es) |
| JP (1) | JPH0665317B2 (es) |
| KR (1) | KR940004543B1 (es) |
| AT (1) | ATE44046T1 (es) |
| AU (1) | AU600229B2 (es) |
| CA (1) | CA1321963C (es) |
| DE (2) | DE3514113A1 (es) |
| DK (1) | DK172695B1 (es) |
| ES (2) | ES8704541A1 (es) |
| FI (1) | FI84362C (es) |
| GR (1) | GR861022B (es) |
| HU (1) | HU196458B (es) |
| IE (1) | IE58994B1 (es) |
| IL (1) | IL78529A (es) |
| NO (1) | NO175646C (es) |
| NZ (1) | NZ215858A (es) |
| PH (1) | PH26596A (es) |
| PT (1) | PT82417B (es) |
| ZA (1) | ZA862925B (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4966849A (en) * | 1985-09-20 | 1990-10-30 | President And Fellows Of Harvard College | CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression |
| AU8379991A (en) * | 1990-09-14 | 1992-03-26 | Astra Aktiebolag | A novel method of generating clones for the expression of unfused proteins |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3247922A1 (de) * | 1982-12-24 | 1984-06-28 | Boehringer Ingelheim International GmbH, 6507 Ingelheim | Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten |
| JPS60188077A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド |
| DE3430683A1 (de) * | 1984-08-21 | 1986-03-06 | Hoechst Ag, 6230 Frankfurt | Synthetische regulationsregion |
-
1985
- 1985-04-19 DE DE19853514113 patent/DE3514113A1/de not_active Withdrawn
-
1986
- 1986-04-09 AT AT86104879T patent/ATE44046T1/de not_active IP Right Cessation
- 1986-04-09 DE DE8686104879T patent/DE3663956D1/de not_active Expired
- 1986-04-09 EP EP86104879A patent/EP0198415B1/de not_active Expired
- 1986-04-17 ES ES554097A patent/ES8704541A1/es not_active Expired
- 1986-04-17 IL IL78529A patent/IL78529A/xx not_active IP Right Cessation
- 1986-04-17 NZ NZ215858A patent/NZ215858A/xx unknown
- 1986-04-17 HU HU861613A patent/HU196458B/hu unknown
- 1986-04-17 FI FI861624A patent/FI84362C/fi not_active IP Right Cessation
- 1986-04-17 PT PT82417A patent/PT82417B/pt unknown
- 1986-04-17 GR GR861022A patent/GR861022B/el unknown
- 1986-04-17 PH PH33672A patent/PH26596A/en unknown
- 1986-04-18 AU AU56387/86A patent/AU600229B2/en not_active Expired
- 1986-04-18 IE IE103186A patent/IE58994B1/en not_active IP Right Cessation
- 1986-04-18 JP JP61089832A patent/JPH0665317B2/ja not_active Expired - Lifetime
- 1986-04-18 DK DK198601796A patent/DK172695B1/da not_active IP Right Cessation
- 1986-04-18 CA CA000507096A patent/CA1321963C/en not_active Expired - Lifetime
- 1986-04-18 ZA ZA862925A patent/ZA862925B/xx unknown
- 1986-04-18 NO NO861551A patent/NO175646C/no unknown
- 1986-04-19 KR KR1019860003040A patent/KR940004543B1/ko not_active Expired - Lifetime
- 1986-06-19 ES ES556233A patent/ES8706818A1/es not_active Expired
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD2A | Announcement of lapse in spain |
Effective date: 20151230 |