[go: up one dir, main page]

ES8605033A1 - Procedimiento para la expresion de genes heterologos en e. coli - Google Patents

Procedimiento para la expresion de genes heterologos en e. coli

Info

Publication number
ES8605033A1
ES8605033A1 ES546242A ES546242A ES8605033A1 ES 8605033 A1 ES8605033 A1 ES 8605033A1 ES 546242 A ES546242 A ES 546242A ES 546242 A ES546242 A ES 546242A ES 8605033 A1 ES8605033 A1 ES 8605033A1
Authority
ES
Spain
Prior art keywords
promotor
region
coli
ribosome
pairs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546242A
Other languages
English (en)
Other versions
ES546242A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES546242A0 publication Critical patent/ES546242A0/es
Publication of ES8605033A1 publication Critical patent/ES8605033A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PROCEDIMIENTO PARA LA EXPRESION DE GENES HETEROLOGOS EN E. COLI. CONSISTE EN COLOCAR DELANTE DEL GEN HETEROLOGO EN LA DIRECCION DE LECTURA UNA REGULACION SINTETICA QUE CONTIENE: UN PROMOTOR CUYA REGION DECIMA TIENE SECUENCIA DE NUCLEOTIDOS DE HELICE CODIFICADORA (CTATAAT) Y CUYA REGION TRIGESIMOQUINTA TIENE LA SECUENCIA DE NUCLEOTIDOS DE HELICE CODIFICADORA (TTGACA); UN OPERADOR LAC MODIFICADO; UN LUGAR DE FIJACION HORMONAL; UNA AGRUPACION ESPACIADORA SITUADA ENTRE LAS REGIONES DECIMA Y TRIGESIMAQUINTA DEL PROMOTOR Y DOTADA DE 16 A 19 PARES DE BASES Y UNA AGRUPACION ESPACIADORA SITUADA ENTRE EL LUGAR DE FIJACION HORMONAL Y EL CORDON DE INICIACION ATG Y DOTADA DE 6 A 14 PARES DE BASES.
ES546242A 1984-08-21 1985-08-19 Procedimiento para la expresion de genes heterologos en e. coli Expired ES8605033A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843430683 DE3430683A1 (de) 1984-08-21 1984-08-21 Synthetische regulationsregion

Publications (2)

Publication Number Publication Date
ES546242A0 ES546242A0 (es) 1986-03-01
ES8605033A1 true ES8605033A1 (es) 1986-03-01

Family

ID=6243523

Family Applications (1)

Application Number Title Priority Date Filing Date
ES546242A Expired ES8605033A1 (es) 1984-08-21 1985-08-19 Procedimiento para la expresion de genes heterologos en e. coli

Country Status (13)

Country Link
EP (1) EP0173149B1 (es)
JP (1) JPS6158591A (es)
KR (1) KR870002259A (es)
AT (1) ATE46538T1 (es)
AU (1) AU592062B2 (es)
DE (2) DE3430683A1 (es)
DK (1) DK378385A (es)
ES (1) ES8605033A1 (es)
FI (1) FI853177A7 (es)
GR (1) GR852000B (es)
HU (1) HU197353B (es)
PT (1) PT80989B (es)
ZA (1) ZA856304B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3514113A1 (de) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression
FR2598430B1 (fr) * 1986-05-06 1990-02-02 Roussel Uclaf Nouveaux vecteurs d'expression, leur procede d'obtention et leur application pour exprimer une proteine quelconque dans escherichia coli
JP2970811B2 (ja) * 1987-10-30 1999-11-02 オンコゲン ア リミテッド パートナーシップ 原核生物細胞中にポリペプチドを調製するための発現システム
US5268284A (en) * 1988-03-11 1993-12-07 The Upjohn Company Ribosome binding site
AU3341089A (en) * 1988-03-11 1989-10-05 Upjohn Company, The Ribosome binding site
GR1002113B (en) * 1988-09-02 1996-02-02 Oncogen Expression systems for preparation of polypeptides in prokaryotic cells
US6689604B1 (en) * 1998-03-20 2004-02-10 National Research Council Of Canada Lipopolysaccharide α-2,3 sialyltransferase of Campylobacter jejuni and its uses

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL65775A (en) * 1981-05-18 1985-05-31 Genentech Inc Microbial hybrid promotors and plasmids and e.coli comprising them
EP0121569B1 (en) * 1982-10-08 1990-08-22 Kyowa Hakko Kogyo Co., Ltd. Novel vector
GR81701B (es) * 1983-01-18 1984-12-12 Lilly Co Eli
AU3235584A (en) * 1983-08-26 1985-02-28 E.I. Du Pont De Nemours And Company Expression plasmids for directing overproduction of specific proteins

Also Published As

Publication number Publication date
KR870002259A (ko) 1987-03-30
AU592062B2 (en) 1990-01-04
EP0173149A1 (de) 1986-03-05
JPS6158591A (ja) 1986-03-25
FI853177L (fi) 1986-02-22
DK378385A (da) 1986-02-22
PT80989B (pt) 1987-11-11
ZA856304B (en) 1986-04-30
GR852000B (es) 1985-12-18
EP0173149B1 (de) 1989-09-20
ES546242A0 (es) 1986-03-01
HU197353B (en) 1989-03-28
DK378385D0 (da) 1985-08-20
ATE46538T1 (de) 1989-10-15
DE3430683A1 (de) 1986-03-06
HUT39207A (en) 1986-08-28
DE3573134D1 (en) 1989-10-26
AU4646885A (en) 1986-02-27
FI853177A7 (fi) 1986-02-22
FI853177A0 (fi) 1985-08-19
PT80989A (de) 1985-09-01

Similar Documents

Publication Publication Date Title
SE8700300D0 (sv) Forfarande for uttryck av gener i vexter
ATE41784T1 (de) Pflanzengen-expression.
EP0028033A3 (en) Novel dna, cloned dna, recombinant plasmid containing the dna, microorganism containing the recombinant plasmid and process for their production
DK453782A (da) Dna-sekvenser, rekombinant plasmid samt anvendelser deraf
FI810722A7 (fi) Menetelmä ja vektoriorganismi, joiden avulla kloonattujen heterologisten geenien tuotteita voidaan valmistaasuuria määriä valvotuissa olosuhteissa Bacillus subtiliksessa.
EP0123811A3 (en) The use of the gal 1 yeast promoter
NZ243500A (en) Nucleic acid encoding dihydrokaempferol hydroxylating enzyme (dhk) and its use in producing transgenic flowering plants
FR2518572B1 (fr) Plasmide d'expression permettant l'insertion appropriee d'adn heterologue
DE3584693D1 (de) Verfahren zur herstellung von kohlenstoffasern des pechtyps.
OA06823A (fr) Procédé d'élaboration d'acier inoxydable.
ES8605033A1 (es) Procedimiento para la expresion de genes heterologos en e. coli
FR2541686B1 (fr) Composition et procede pour la transformation genetique de plantes dicotyledones, et nouvelle souche d'agrobacterium rhizogenes utilisee a cet effet
ATE27308T1 (de) Duesentraeger aus feuerfestem material sowie verfahren zur verminderung seines verschleisses waehrend der benutzung.
DE3585695D1 (de) Expressionsplasmide die vollstaendige cdna-sequenz von kalbsprochymosin enthaltend.
IT8521507A0 (it) Vettore di clonaggio, molecole di dna ricombinante, ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni.
ZA851996B (en) Purification,cloning and characterization of the gene for interleukin 1
DE3573256D1 (en) (6,11-dihydro-11-oxodibenz(b,e)oxepinyl)pentanoic acids and derivatives, a process and intermediates for their preparation and their use as medicaments
IT8220743V0 (it) Barra di acciaio inossidabile ad aderenza migliorata per l'armatura del calcestruzzo.
PT81902B (pt) Processo de obtencao dum derivado de amida do acido 1,3-benzenodicarboxilico
IT7812735A0 (it) Fiaggio di ossigeno per la produzio procedimento per il controllo dinane dell'acciaio mico del carbonio durante il sof-
IT1181456B (it) Macchina diamantatrice per l'industria orafa