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ES509946A0 - Un metodo para aumentar la produccion de proteinas por adn heterologo, en vectores de clonacion de plasmidos. - Google Patents

Un metodo para aumentar la produccion de proteinas por adn heterologo, en vectores de clonacion de plasmidos.

Info

Publication number
ES509946A0
ES509946A0 ES509946A ES509946A ES509946A0 ES 509946 A0 ES509946 A0 ES 509946A0 ES 509946 A ES509946 A ES 509946A ES 509946 A ES509946 A ES 509946A ES 509946 A0 ES509946 A0 ES 509946A0
Authority
ES
Spain
Prior art keywords
proteins
production
increase
cloning vectors
plasmid cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES509946A
Other languages
English (en)
Other versions
ES8305415A1 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of ES509946A0 publication Critical patent/ES509946A0/es
Publication of ES8305415A1 publication Critical patent/ES8305415A1/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/69Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ES509946A 1981-02-27 1982-02-26 Un metodo para aumentar la produccion de proteinas por adn heterologo, en vectores de clonacion de plasmidos. Expired ES8305415A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23916581A 1981-02-27 1981-02-27

Publications (2)

Publication Number Publication Date
ES509946A0 true ES509946A0 (es) 1983-04-01
ES8305415A1 ES8305415A1 (es) 1983-04-01

Family

ID=22900899

Family Applications (1)

Application Number Title Priority Date Filing Date
ES509946A Expired ES8305415A1 (es) 1981-02-27 1982-02-26 Un metodo para aumentar la produccion de proteinas por adn heterologo, en vectores de clonacion de plasmidos.

Country Status (11)

Country Link
EP (1) EP0060045A3 (es)
JP (1) JPS57172000A (es)
AU (1) AU554700B2 (es)
BR (1) BR8201015A (es)
CA (1) CA1198067A (es)
DK (1) DK85682A (es)
ES (1) ES8305415A1 (es)
FI (1) FI820688L (es)
IL (1) IL65061A (es)
NO (1) NO820610L (es)
WO (1) WO1982002901A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1198069A (en) * 1982-06-08 1985-12-17 David H. Gelfand Temperature sensitive multicopy plasmids
CA1209501A (en) * 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
WO1984001171A1 (en) * 1982-09-16 1984-03-29 Benzon As Alfred Plasmids with conditional uncontrolled replication behaviour
NL8203716A (nl) * 1982-09-24 1984-04-16 Tno Dna en plasmiden.
GB8308236D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Dna vectors
US5670371A (en) * 1983-07-15 1997-09-23 Bio-Technology General Corp. Bacterial expression of superoxide dismutase
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
DE3585170D1 (de) 1984-03-06 1992-02-27 Lilly Co Eli Expressionsvektoren fuer rinderwachstumshormonderivate.
US4753886A (en) * 1984-08-10 1988-06-28 Eli Lilly And Company Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes
GB2166743B (en) * 1984-11-13 1989-01-05 Solvay Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression
JP4972412B2 (ja) 2004-02-04 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション E.コリ発酵によるプラスミドdnaの大規模生産のための方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4322499A (en) * 1978-12-22 1982-03-30 The Regents Of The University Of California Adrenocorticotropin-lipotropin precursor gene
FI793884A7 (fi) * 1978-12-26 1981-01-01 Cetus Corp Vakaita runsaasti jäljentyviä plasmideja.

Also Published As

Publication number Publication date
CA1198067A (en) 1985-12-17
WO1982002901A1 (en) 1982-09-02
EP0060045A3 (en) 1983-08-24
FI820688A7 (fi) 1982-08-28
ES8305415A1 (es) 1983-04-01
DK85682A (da) 1982-08-28
IL65061A (en) 1986-01-31
FI820688L (fi) 1982-08-28
EP0060045A2 (en) 1982-09-15
JPS57172000A (en) 1982-10-22
IL65061A0 (en) 1982-04-30
NO820610L (no) 1982-08-30
AU554700B2 (en) 1986-08-28
AU8274782A (en) 1982-09-14
BR8201015A (pt) 1983-01-04

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