ES485047A1 - Support Matrices for Immobilized Enzymes - Google Patents
Support Matrices for Immobilized EnzymesInfo
- Publication number
- ES485047A1 ES485047A1 ES485047A ES485047A ES485047A1 ES 485047 A1 ES485047 A1 ES 485047A1 ES 485047 A ES485047 A ES 485047A ES 485047 A ES485047 A ES 485047A ES 485047 A1 ES485047 A1 ES 485047A1
- Authority
- ES
- Spain
- Prior art keywords
- support
- copolymeric
- immobilized enzymes
- aminopolystyrene
- pores
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108010093096 Immobilized Enzymes Proteins 0.000 title abstract 3
- 229920001577 copolymer Polymers 0.000 abstract 3
- 230000001588 bifunctional effect Effects 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 239000000178 monomer Substances 0.000 abstract 2
- 239000011148 porous material Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 238000000151 deposition Methods 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 239000011368 organic material Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F8/00—Chemical modification by after-treatment
- C08F8/04—Reduction, e.g. hydrogenation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Organic-inorganic support matrices for immobilized enzymes comprise a solid, porous, inorganic, water insoluble support and a copolymeric material resulting from the reaction of aminopolystyrene and a bifunctional monomer. The copolymeric material is substantially entrapped in the pores of the support and contains functionalized pendent groups to which an enzyme may be coupled to form the immobilized enzyme conjugate. The matrix may be prepared by depositing a salt of aminopolystyrene within the pores of the solid support from an aqueous solution at a pH less than 7 after which the resulting composite is then reacted with an excess of a bifunctional reactive monomer thus forming the copolymeric organic material in situ.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/951,949 US4206259A (en) | 1978-10-16 | 1978-10-16 | Support matrices for immobilized enzymes |
| US05/951,948 US4193910A (en) | 1978-10-16 | 1978-10-16 | Preparation of support matrices for immobilized enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES485047A1 true ES485047A1 (en) | 1980-05-16 |
Family
ID=27130323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES485047A Expired ES485047A1 (en) | 1978-10-16 | 1979-10-16 | Support Matrices for Immobilized Enzymes |
Country Status (7)
| Country | Link |
|---|---|
| CA (1) | CA1128917A (en) |
| DE (1) | DE2941881C2 (en) |
| DK (1) | DK436379A (en) |
| ES (1) | ES485047A1 (en) |
| FR (1) | FR2453898A1 (en) |
| GB (1) | GB2033399B (en) |
| IT (1) | IT1125483B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0062968B2 (en) * | 1981-03-18 | 1991-07-10 | FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. | Support material for use in serological testing and process for the production thereof |
| DE3130924A1 (en) * | 1981-08-05 | 1983-02-17 | Röhm GmbH, 6100 Darmstadt | SURFACE-BASED SYSTEMS FOR FIXING SUBSTRATES CONTAINING NUCLEOPHILE GROUPS |
| CN113061600A (en) * | 2021-03-05 | 2021-07-02 | 宁波泓森生物科技有限公司 | Preparation method of immobilized threonine aldolase, immobilized threonine aldolase and application |
| CN114540332B (en) * | 2022-03-01 | 2023-07-21 | 江苏恰瑞生物科技有限公司 | Preparation method and application of uricase immobilized resin |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3860486A (en) * | 1973-05-24 | 1975-01-14 | Owens Illinois Inc | Insolubilizing enzymes with polystyrene derivatives |
| GB1485122A (en) * | 1973-08-06 | 1977-09-08 | Nat Res Dev | Biologically active matrices |
| US4001085A (en) * | 1973-09-10 | 1977-01-04 | Owens-Illinois, Inc. | Immobilization of enzymes on an inorganic matrix |
| GB1485123A (en) * | 1974-07-25 | 1977-09-08 | Nat Res Dev | Support for biologically active material |
| PT64790B (en) * | 1975-02-18 | 1977-07-07 | Uop Inc | Immobilized enzyme conjugates |
| US4141857A (en) * | 1976-04-30 | 1979-02-27 | Uop Inc. | Support matrices for immobilized enzymes |
-
1979
- 1979-10-15 CA CA337,572A patent/CA1128917A/en not_active Expired
- 1979-10-16 ES ES485047A patent/ES485047A1/en not_active Expired
- 1979-10-16 GB GB7935953A patent/GB2033399B/en not_active Expired
- 1979-10-16 IT IT26535/79A patent/IT1125483B/en active
- 1979-10-16 FR FR7925672A patent/FR2453898A1/en active Granted
- 1979-10-16 DK DK436379A patent/DK436379A/en not_active Application Discontinuation
- 1979-10-16 DE DE2941881A patent/DE2941881C2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB2033399B (en) | 1982-11-24 |
| DE2941881A1 (en) | 1980-04-17 |
| IT1125483B (en) | 1986-05-14 |
| CA1128917A (en) | 1982-08-03 |
| IT7926535A0 (en) | 1979-10-16 |
| FR2453898B1 (en) | 1983-11-18 |
| DK436379A (en) | 1980-04-17 |
| FR2453898A1 (en) | 1980-11-07 |
| DE2941881C2 (en) | 1983-11-10 |
| GB2033399A (en) | 1980-05-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 19990601 |