ES457925A1 - A PROCEDURE FOR THE PREPARATION OF AN ENZYME ALPHA-AMI-LASA STABLE TO HEAT AND TO ACIDS. - Google Patents
A PROCEDURE FOR THE PREPARATION OF AN ENZYME ALPHA-AMI-LASA STABLE TO HEAT AND TO ACIDS.Info
- Publication number
- ES457925A1 ES457925A1 ES457925A ES457925A ES457925A1 ES 457925 A1 ES457925 A1 ES 457925A1 ES 457925 A ES457925 A ES 457925A ES 457925 A ES457925 A ES 457925A ES 457925 A1 ES457925 A1 ES 457925A1
- Authority
- ES
- Spain
- Prior art keywords
- heat
- lasa
- ami
- acids
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002253 acid Substances 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 150000007513 acids Chemical class 0.000 title 1
- 102000004139 alpha-Amylases Human genes 0.000 abstract 4
- 108090000637 alpha-Amylases Proteins 0.000 abstract 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract 3
- 229940024171 alpha-amylase Drugs 0.000 abstract 3
- 229910001424 calcium ion Inorganic materials 0.000 abstract 3
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Abstract
Una enzima alfa-amilasa estable al calor y a los ácidos caracterizado porque (1) es capaz de retener por lo menos aproximadamente 70% de su actividad inicial de alfa-amilasa cuando se mantiene a 90ºC y a un pH de 6,0 durante 10 minutos en ausencia de iones calcio añadidos; (2) es capaz de retener por lo menos aproximadamente 50% de su actividad inicial de alfa-amilasa cuando se mantiene a 90ºC a un pH de 6,0 durante 60 minutos en ausencia de iones calcio añadidos; y (3) es capaz de retener por lo menos aproximadamente 50% de retener por lo menos aproximadamente 50% de su actividad inicial de alfa-amilasa a una temperatura de 80ºC y a un pH de 4,55 en presencia de 5mM de iones calcio durante 10 minutos.A heat and acid stable alpha-amylase enzyme characterized in that (1) it is capable of retaining at least about 70% of its initial alpha-amylase activity when maintained at 90° C. and a pH of 6.0 for 10 minutes in the absence of added calcium ions; (2) it is capable of retaining at least about 50% of its initial alpha-amylase activity when maintained at 90° C. and a pH of 6.0 for 60 minutes in the absence of added calcium ions; and (3) it is capable of retaining at least about 50% of its initial alpha-amylase activity at a temperature of 80° C. and a pH of 4.55 in the presence of 5 mM calcium ions for 10 minutes.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67851376A | 1976-04-19 | 1976-04-19 | |
| US76492377A | 1977-02-02 | 1977-02-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES457925A1 true ES457925A1 (en) | 1978-03-01 |
Family
ID=27102035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES457925A Expired ES457925A1 (en) | 1976-04-19 | 1977-04-18 | A PROCEDURE FOR THE PREPARATION OF AN ENZYME ALPHA-AMI-LASA STABLE TO HEAT AND TO ACIDS. |
Country Status (16)
| Country | Link |
|---|---|
| JP (1) | JPS52151790A (en) |
| AR (1) | AR218023A1 (en) |
| AU (1) | AU513863B2 (en) |
| BR (1) | BR7702475A (en) |
| CA (1) | CA1081633A (en) |
| DE (1) | DE2717333C2 (en) |
| DK (1) | DK149157C (en) |
| ES (1) | ES457925A1 (en) |
| FI (1) | FI58157C (en) |
| FR (1) | FR2348930A1 (en) |
| GB (1) | GB1539694A (en) |
| IT (1) | IT1080353B (en) |
| MY (1) | MY8000095A (en) |
| NL (1) | NL7704280A (en) |
| NZ (1) | NZ183818A (en) |
| YU (1) | YU40476B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA82133B (en) * | 1981-01-15 | 1983-02-23 | Cpc International Inc | A process for cloning the gene coding for a thermostabic alpha amylase into escherichia coli and bacillus subtilis |
| US4376824A (en) * | 1981-04-27 | 1983-03-15 | Nabisco Brands, Inc. | Process for producing glucose/fructose syrups from unrefined starch hydrolysates |
| GB2129806B (en) * | 1982-10-29 | 1986-04-03 | Cpc International Inc | Process for preparing high-dextrose starch hydrolysates with immobilized glucoamylase |
| GB8414272D0 (en) * | 1984-06-05 | 1984-07-11 | Cpc International Inc | Enzymatic hydrolysis |
| US5010008A (en) * | 1988-12-12 | 1991-04-23 | Enzyme Bio-Systems Ltd. | Stable liquid enzyme concentrate and process for its production |
| JP2019094420A (en) * | 2017-11-22 | 2019-06-20 | 株式会社ニイタカ | Detergent composition, detergent for immerse and detergent for dishwasher |
| CN110423737B (en) * | 2019-09-10 | 2021-04-30 | 白银赛诺生物科技有限公司 | Thermostable α-amylase derived from Geobacillus stearothermophilus and its application |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2695863A (en) * | 1952-04-16 | 1954-11-30 | Purdue Research Foundation | Process for preparing alpha amylase |
| GB1285173A (en) * | 1968-09-09 | 1972-08-09 | Glaxo Lab Ltd | AN alpha-AMYLASE AND A PROCESS FOR ITS PREPARATION |
| FR2256248A1 (en) * | 1973-12-28 | 1975-07-25 | Staley Mfg Co A E | Non-retrograded thinned starch hydrolyzates - with reduced alpha amylase requirements |
| JPS5144690A (en) * | 1974-10-14 | 1976-04-16 | Nagase & Co Ltd | Tainetsuseiarufua amiraazeno seizoho |
| FR2304675A1 (en) * | 1975-03-21 | 1976-10-15 | Cpc International Inc | Granular starch conversion to fructose - in water using alpha-amylase, glucamylase and glucose isomerase mixt. under non-gelatinising conditions |
-
1977
- 1977-04-06 NZ NZ183818A patent/NZ183818A/en unknown
- 1977-04-13 AU AU24226/77A patent/AU513863B2/en not_active Expired
- 1977-04-15 YU YU998/77A patent/YU40476B/en unknown
- 1977-04-18 GB GB16029/77A patent/GB1539694A/en not_active Expired
- 1977-04-18 CA CA276,395A patent/CA1081633A/en not_active Expired
- 1977-04-18 DK DK169877A patent/DK149157C/en not_active IP Right Cessation
- 1977-04-18 ES ES457925A patent/ES457925A1/en not_active Expired
- 1977-04-19 BR BR7702475A patent/BR7702475A/en unknown
- 1977-04-19 AR AR267261A patent/AR218023A1/en active
- 1977-04-19 FR FR7711773A patent/FR2348930A1/en active Granted
- 1977-04-19 IT IT22615/77A patent/IT1080353B/en active
- 1977-04-19 FI FI771240A patent/FI58157C/en not_active IP Right Cessation
- 1977-04-19 NL NL7704280A patent/NL7704280A/en not_active Application Discontinuation
- 1977-04-19 DE DE2717333A patent/DE2717333C2/en not_active Expired
- 1977-04-19 JP JP4416877A patent/JPS52151790A/en active Granted
-
1980
- 1980-12-30 MY MY95/80A patent/MY8000095A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA1081633A (en) | 1980-07-15 |
| DE2717333A1 (en) | 1977-11-10 |
| DK169877A (en) | 1977-10-20 |
| JPS52151790A (en) | 1977-12-16 |
| AR218023A1 (en) | 1980-05-15 |
| BR7702475A (en) | 1978-05-02 |
| DE2717333C2 (en) | 1986-10-16 |
| NZ183818A (en) | 1980-05-08 |
| IT1080353B (en) | 1985-05-16 |
| JPS5752837B2 (en) | 1982-11-10 |
| YU99877A (en) | 1982-08-31 |
| FI58157B (en) | 1980-08-29 |
| FR2348930B1 (en) | 1983-02-11 |
| DK149157C (en) | 1986-07-14 |
| YU40476B (en) | 1986-02-28 |
| NL7704280A (en) | 1977-10-21 |
| AU2422677A (en) | 1978-10-19 |
| MY8000095A (en) | 1980-12-31 |
| DK149157B (en) | 1986-02-17 |
| AU513863B2 (en) | 1981-01-08 |
| FI58157C (en) | 1980-12-10 |
| FI771240A7 (en) | 1977-10-20 |
| GB1539694A (en) | 1979-01-31 |
| FR2348930A1 (en) | 1977-11-18 |
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