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ES439201A1 - Cell and virus culture systems - Google Patents

Cell and virus culture systems

Info

Publication number
ES439201A1
ES439201A1 ES439201A ES439201A ES439201A1 ES 439201 A1 ES439201 A1 ES 439201A1 ES 439201 A ES439201 A ES 439201A ES 439201 A ES439201 A ES 439201A ES 439201 A1 ES439201 A1 ES 439201A1
Authority
ES
Spain
Prior art keywords
cells
bed
microorganism
vaccine
preparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES439201A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wellcome Foundation Ltd
Original Assignee
Wellcome Foundation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wellcome Foundation Ltd filed Critical Wellcome Foundation Ltd
Publication of ES439201A1 publication Critical patent/ES439201A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
    • C12N2770/32151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

An improved procedure for preparing a vaccine by means of a live eukaryotic cell culture system, comprising the steps of: (a) preparing a support bed or starting from a suspension of porous or particulate material, (b) applying a liquid suspension of eukaryotic cells to the bed, (c) allowing the cells retained in the bed to establish themselves, (d) circulating nutrient medium through the bed, whereby nutrients are supplied to the cells and separated metabolic and degradation products in the fluid leaving the bed, (e) infecting the cells with a microorganism to which the cells are susceptible, (f) cultivating the microorganism in a nutrient medium, (g) separating the microorganism from the waste from the cells, (h) if necessary, remove bacterial contamination by filtration, and (i) formulate the microorganisms in a vaccine in a known manner. (Machine-translation by Google Translate, not legally binding)
ES439201A 1972-04-26 1975-07-07 Cell and virus culture systems Expired ES439201A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1938772A GB1436323A (en) 1972-04-26 1972-04-26 Cell and virus culture systems

Publications (1)

Publication Number Publication Date
ES439201A1 true ES439201A1 (en) 1977-05-16

Family

ID=10128513

Family Applications (2)

Application Number Title Priority Date Filing Date
ES414067A Expired ES414067A1 (en) 1972-04-26 1973-04-25 Cell and virus culture systems
ES439201A Expired ES439201A1 (en) 1972-04-26 1975-07-07 Cell and virus culture systems

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES414067A Expired ES414067A1 (en) 1972-04-26 1973-04-25 Cell and virus culture systems

Country Status (12)

Country Link
JP (2) JPS5925597B2 (en)
AR (1) AR200261A1 (en)
BE (1) BE798703A (en)
DE (1) DE2320885C2 (en)
DK (1) DK141911B (en)
ES (2) ES414067A1 (en)
FR (1) FR2182049B1 (en)
GB (1) GB1436323A (en)
IN (1) IN139615B (en)
IT (1) IT1040527B (en)
NL (1) NL7305777A (en)
ZA (1) ZA732813B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
DE3065415D1 (en) * 1979-06-28 1983-12-01 Thomae Gmbh Dr K Process for surface growing of nucleus-containing cells and production of cell culture-dependent products
BE1025089B1 (en) 2017-03-30 2018-10-29 Univercells Sa CLARIFICATION OF CELL CULTURE

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1517758A1 (en) * 1966-11-02 1970-01-29 Intermag Ag Method for the quantitative fixation and dosage of microorganisms
US3580811A (en) * 1968-04-16 1971-05-25 Commercial Solvents Corp Synthetic fermentation medium and process using same for cultivating gibberella zeae
FR2053565A5 (en) * 1969-07-09 1971-04-16 Pasteur Institut

Also Published As

Publication number Publication date
ZA732813B (en) 1974-12-24
JPS5794291A (en) 1982-06-11
JPS5925597B2 (en) 1984-06-19
NL7305777A (en) 1973-10-30
FR2182049A1 (en) 1973-12-07
DK141911B (en) 1980-07-14
ES414067A1 (en) 1976-06-16
GB1436323A (en) 1976-05-19
JPS5825645B2 (en) 1983-05-28
DK141911C (en) 1980-11-24
JPS4947581A (en) 1974-05-08
IN139615B (en) 1976-07-10
DE2320885C2 (en) 1985-06-27
IT1040527B (en) 1979-12-20
FR2182049B1 (en) 1976-11-12
BE798703A (en) 1973-10-25
DE2320885A1 (en) 1973-11-15
AR200261A1 (en) 1974-10-31

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