ES262178A1 - METHOD FOR OBTAINING A POLYVALENT GONADOTROPIC HORMONE FREE OF ANTIGENIC PROPERTIES - Google Patents
METHOD FOR OBTAINING A POLYVALENT GONADOTROPIC HORMONE FREE OF ANTIGENIC PROPERTIESInfo
- Publication number
- ES262178A1 ES262178A1 ES0262178A ES262178A ES262178A1 ES 262178 A1 ES262178 A1 ES 262178A1 ES 0262178 A ES0262178 A ES 0262178A ES 262178 A ES262178 A ES 262178A ES 262178 A1 ES262178 A1 ES 262178A1
- Authority
- ES
- Spain
- Prior art keywords
- hormone
- precipitate
- washing
- ethanol
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 229940088597 hormone Drugs 0.000 title abstract 8
- 239000005556 hormone Substances 0.000 title abstract 8
- 230000001456 gonadotroph Effects 0.000 title abstract 2
- 230000000890 antigenic effect Effects 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- 238000005406 washing Methods 0.000 abstract 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 239000005995 Aluminium silicate Substances 0.000 abstract 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 abstract 2
- 239000005695 Ammonium acetate Substances 0.000 abstract 2
- 235000012211 aluminium silicate Nutrition 0.000 abstract 2
- 229940043376 ammonium acetate Drugs 0.000 abstract 2
- 235000019257 ammonium acetate Nutrition 0.000 abstract 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract 1
- 229910021536 Zeolite Inorganic materials 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 125000002091 cationic group Chemical group 0.000 abstract 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 230000003397 luteinic effect Effects 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 239000010457 zeolite Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
A gonadotropic hormone active both as a follicle-stimulant hormone, and as a luteinic hormone and devoid of antigen properties, is prepared by treating fresh urine obtained from women in menopausal or post-menopausal stage, at a pH of 4 to 4.5 with kaolin purified by washing with dilute mineral acid, separating the kaolin with adsorbed hormone, washing, extracting with aqueous ammonia of pH 11 to 11.3, centrifuging and adjusting the pH of the liquid so obtained to pH 8.5 by the addition of acetic acid, discarding the precipitate, lowering the pH of the residual liquid to pH 5 to 5.5 and adding acetone to precipitate the crude hormone which is further purified, repeated if necessary, by chromatographic adsorption on a cationic exchange zeolite, previously acidified to a pH of 3.7 to 3.8, followed by washing with water to neutrality, next with aqueous ethanol of 70 to 80% concentration, then with aqueous ethanol of 70 to 80% concentration containing ammonium acetate and finally eluting with aqueous ethanol of 35 to 45% concentration containing ammonium acetate, the hormone being precipitated from the eluate by increasing the ethanol content of the eluate, and is then washed and dried. It is preferred to precipitate the hormone by adjusting to an ethanol concentration of 80%.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT964060 | 1960-05-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES262178A1 true ES262178A1 (en) | 1961-01-01 |
Family
ID=11132851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES0262178A Expired ES262178A1 (en) | 1960-05-31 | 1960-11-04 | METHOD FOR OBTAINING A POLYVALENT GONADOTROPIC HORMONE FREE OF ANTIGENIC PROPERTIES |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES262178A1 (en) |
| GB (1) | GB903346A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013259551B2 (en) * | 2012-05-09 | 2017-11-02 | Sunovion Pharmaceuticals Inc. | Heteroaryl compounds and methods of use thereof |
-
1960
- 1960-10-14 GB GB3525860A patent/GB903346A/en not_active Expired
- 1960-11-04 ES ES0262178A patent/ES262178A1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB903346A (en) | 1962-08-15 |
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