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ES2532030A1 - Method of measuring ex vivo vascular tone and flow chamber (Machine-translation by Google Translate, not legally binding) - Google Patents

Method of measuring ex vivo vascular tone and flow chamber (Machine-translation by Google Translate, not legally binding) Download PDF

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Publication number
ES2532030A1
ES2532030A1 ES201300866A ES201300866A ES2532030A1 ES 2532030 A1 ES2532030 A1 ES 2532030A1 ES 201300866 A ES201300866 A ES 201300866A ES 201300866 A ES201300866 A ES 201300866A ES 2532030 A1 ES2532030 A1 ES 2532030A1
Authority
ES
Spain
Prior art keywords
vascular tone
flow chamber
measuring
vessel
vivo vascular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201300866A
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Spanish (es)
Other versions
ES2532030B2 (en
Inventor
María Teresa TEJERINA SÁNCHEZ
Santiago REDONDO BLASCO
Úrsula Fabiola MEDINA MORENO
Jorge NAVARRO DORADO
Marta RAMAJO MATESANZ
Alexis CANTIZANO GONZÁLEZ
Mario CASTRO PONCE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Complutense de Madrid
Original Assignee
Universidad Complutense de Madrid
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Publication date
Application filed by Universidad Complutense de Madrid filed Critical Universidad Complutense de Madrid
Priority to ES201300866A priority Critical patent/ES2532030B2/en
Publication of ES2532030A1 publication Critical patent/ES2532030A1/en
Application granted granted Critical
Publication of ES2532030B2 publication Critical patent/ES2532030B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método de medición del tono vascular ex vivo y cámara de flujo. La presente invención se refiere a un método de medición del tono vascular ex vivo que comprende: perfundir un flujo constante de una suspensión celular en un circuito abierto que incluye un vaso cuyo tono se desea estudiar, cerrar el circuito, perfundir una solución nutricia de Krebs en el circuito cerrado y aplicar una presión transmural constante, añadir una sustancia vasoactiva y registrar las variaciones de diámetro del vaso mediante un sistema de captación de imágenes situado perpendicularmente al vaso y acoplado a un microscopio. La invención también se refiere a una cámara de flujo en la que realizar el método de medición del tono vascular que incluye dos capilares de vidrio, un baño, una bomba de infusión, un sistema de presión hidrostática, un manómetro, un sistema de termorregulación de la temperatura del baño y de las soluciones y/o suspensiones utilizadas, un sistema de captación de imágenes y un microscopio.Ex vivo vascular tone measurement method and flow chamber. The present invention refers to a method for measuring ex vivo vascular tone that comprises: perfusing a constant flow of a cell suspension in an open circuit that includes a vessel whose tone is to be studied, closing the circuit, perfusing a Krebs nutrient solution in the closed circuit and apply a constant transmural pressure, add a vasoactive substance and record the variations in diameter of the vessel using an image capture system located perpendicular to the vessel and coupled to a microscope. The invention also refers to a flow chamber in which to carry out the vascular tone measurement method that includes two glass capillaries, a bath, an infusion pump, a hydrostatic pressure system, a manometer, a thermoregulation system of the temperature of the bath and of the solutions and/or suspensions used, an image capture system and a microscope.

Description

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Claims (1)

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ES201300866A 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber Active ES2532030B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Publications (2)

Publication Number Publication Date
ES2532030A1 true ES2532030A1 (en) 2015-03-23
ES2532030B2 ES2532030B2 (en) 2015-12-28

Family

ID=52683008

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201300866A Active ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Country Status (1)

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ES (1) ES2532030B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878A (en) * 2023-03-07 2023-05-02 中国人民解放军军事科学院军事医学研究院 Vascular pipe diameter detection equipment

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
US4841974A (en) * 1986-12-22 1989-06-27 Siemens Aktiengesellschaft Apparatus and method for the examination of a liquid medium
WO1990014791A1 (en) * 1989-05-31 1990-12-13 Board Of Regents, The University Of Texas System Apparatus for analysis of biological compounds by mass spectrometry
US20020188240A1 (en) * 2001-05-14 2002-12-12 Gorsuch Reynolds G. Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
WO2006119143A2 (en) * 2005-04-29 2006-11-09 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
US4841974A (en) * 1986-12-22 1989-06-27 Siemens Aktiengesellschaft Apparatus and method for the examination of a liquid medium
WO1990014791A1 (en) * 1989-05-31 1990-12-13 Board Of Regents, The University Of Texas System Apparatus for analysis of biological compounds by mass spectrometry
US20020188240A1 (en) * 2001-05-14 2002-12-12 Gorsuch Reynolds G. Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
WO2006119143A2 (en) * 2005-04-29 2006-11-09 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878A (en) * 2023-03-07 2023-05-02 中国人民解放军军事科学院军事医学研究院 Vascular pipe diameter detection equipment

Also Published As

Publication number Publication date
ES2532030B2 (en) 2015-12-28

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