ES2484665B2 - Electrode device for the detection of gluconic acid, manufacturing process and use of said device - Google Patents
Electrode device for the detection of gluconic acid, manufacturing process and use of said device Download PDFInfo
- Publication number
- ES2484665B2 ES2484665B2 ES201300149A ES201300149A ES2484665B2 ES 2484665 B2 ES2484665 B2 ES 2484665B2 ES 201300149 A ES201300149 A ES 201300149A ES 201300149 A ES201300149 A ES 201300149A ES 2484665 B2 ES2484665 B2 ES 2484665B2
- Authority
- ES
- Spain
- Prior art keywords
- detection
- electrode
- gluconic acid
- active area
- work
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 title abstract 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 title abstract 2
- 235000012208 gluconic acid Nutrition 0.000 title abstract 2
- 239000000174 gluconic acid Substances 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 229940098773 bovine serum albumin Drugs 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- FHCPAXDKURNIOZ-UHFFFAOYSA-N tetrathiafulvalene Chemical compound S1C=CSC1=C1SC=CS1 FHCPAXDKURNIOZ-UHFFFAOYSA-N 0.000 abstract 2
- 101710162174 D-glyceraldehyde dehydrogenase (NADP(+)) Proteins 0.000 abstract 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 abstract 1
- 230000001588 bifunctional effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 229920000728 polyester Polymers 0.000 abstract 1
- 238000007650 screen-printing Methods 0.000 abstract 1
- 229910052709 silver Inorganic materials 0.000 abstract 1
- 239000004332 silver Substances 0.000 abstract 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente invención consiste en un dispositivo electródico para la detección de ácido glucónico, con tres electrodos, uno de referencia, otro de trabajo y otro auxiliar, obtenidos todos ellos por serigrafía, el área activa del electrodo de referencia incluye tinta de plata/cloruro de plata, la del contraelectrodo tinta de carbono y la del electrodo de trabajo con una tinta de carbono que incorpora tetratiafulvaleno (TTF) al 3%, sobre una lámina plástica de poliéster, modificándose el área activa del trabajo con la enzima GADH utilizando un agente bifuncional en presencia de seroalbúmina bovina (BSA), así como su procedimiento de fabricación y su uso en la detección citada, de manera simple, con bajo coste y en relativo corto tiempo de análisis.The present invention consists of an electrode device for the detection of gluconic acid, with three electrodes, one reference, one working and one auxiliary, all obtained by screen printing, the active area of the reference electrode includes silver ink / chloride silver, the carbon ink counter electrode and the work electrode with a carbon ink incorporating 3% tetrathiafulvalene (TTF) on a plastic polyester sheet, modifying the active area of work with the GADH enzyme using a bifunctional agent in the presence of bovine serum albumin (BSA), as well as its manufacturing procedure and its use in the detection mentioned, in a simple way, with low cost and in a relatively short time of analysis.
Description
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201300149A ES2484665B2 (en) | 2013-02-11 | 2013-02-11 | Electrode device for the detection of gluconic acid, manufacturing process and use of said device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201300149A ES2484665B2 (en) | 2013-02-11 | 2013-02-11 | Electrode device for the detection of gluconic acid, manufacturing process and use of said device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2484665A1 ES2484665A1 (en) | 2014-08-11 |
| ES2484665B2 true ES2484665B2 (en) | 2015-03-16 |
Family
ID=51265999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES201300149A Active ES2484665B2 (en) | 2013-02-11 | 2013-02-11 | Electrode device for the detection of gluconic acid, manufacturing process and use of said device |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2484665B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2638737B1 (en) * | 2016-04-22 | 2018-08-14 | Universidad De Burgos | Electrode device and measurement procedure for the detection of lactic acid |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1292823A1 (en) * | 2000-05-23 | 2003-03-19 | Radiometer Medical A/S | A sensor membrane, a method for the preparation thereof, a sensor and a layered membrane structure for such sensor |
| EP1321763B1 (en) * | 2000-09-25 | 2010-01-27 | Asahi Kasei Kabushiki Kaisha | Enzyme electrode |
| DE60125822T2 (en) * | 2000-11-09 | 2007-04-26 | Ird A/S | Method for producing membrane electrode assemblies for use in direct methanol fuel cells |
| CA2699823C (en) * | 2007-09-18 | 2016-10-25 | Ultizyme International Ltd. | Enzyme electrode |
-
2013
- 2013-02-11 ES ES201300149A patent/ES2484665B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2484665A1 (en) | 2014-08-11 |
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|---|---|---|---|
| FG2A | Definitive protection |
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