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ES2174884T3 - EXPANSION OF CHAINS BY DISPLACEMENT USING THERMOPHILIC ENCIMS. - Google Patents

EXPANSION OF CHAINS BY DISPLACEMENT USING THERMOPHILIC ENCIMS.

Info

Publication number
ES2174884T3
ES2174884T3 ES95103569T ES95103569T ES2174884T3 ES 2174884 T3 ES2174884 T3 ES 2174884T3 ES 95103569 T ES95103569 T ES 95103569T ES 95103569 T ES95103569 T ES 95103569T ES 2174884 T3 ES2174884 T3 ES 2174884T3
Authority
ES
Spain
Prior art keywords
thermophilic
sda
found
temperature range
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95103569T
Other languages
Spanish (es)
Inventor
Melinda S Fraiser
Catherine A Spargo
George Terrance Walker
Cleve Mark Van
David James Wright
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2174884T3 publication Critical patent/ES2174884T3/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)

Abstract

Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37 DEG C to 70 DEG C). The preferred temperature range for thermophilic SDA is 50 DEG C to 70 DEG C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products. In addition, the need to add the enzymes in a separate step after the initial heat denaturation of double stranded targets is eliminated when enzymes capable of tolerating the denaturation temperature are used.
ES95103569T 1994-04-18 1995-03-13 EXPANSION OF CHAINS BY DISPLACEMENT USING THERMOPHILIC ENCIMS. Expired - Lifetime ES2174884T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/229,279 US5648211A (en) 1994-04-18 1994-04-18 Strand displacement amplification using thermophilic enzymes

Publications (1)

Publication Number Publication Date
ES2174884T3 true ES2174884T3 (en) 2002-11-16

Family

ID=22860532

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95103569T Expired - Lifetime ES2174884T3 (en) 1994-04-18 1995-03-13 EXPANSION OF CHAINS BY DISPLACEMENT USING THERMOPHILIC ENCIMS.

Country Status (11)

Country Link
US (2) US5648211A (en)
EP (1) EP0684315B1 (en)
JP (1) JP3140937B2 (en)
KR (1) KR0156290B1 (en)
AT (1) ATE219792T1 (en)
AU (1) AU695574B2 (en)
BR (1) BR9501581A (en)
CA (1) CA2144495C (en)
DE (1) DE69527171T2 (en)
ES (1) ES2174884T3 (en)
TW (1) TW408224B (en)

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