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ES2005545A6 - Un procedimiento para la preparacion de una formulacion de sustrato para un ensayo de fosfatasa alcalina. - Google Patents

Un procedimiento para la preparacion de una formulacion de sustrato para un ensayo de fosfatasa alcalina.

Info

Publication number
ES2005545A6
ES2005545A6 ES8700017A ES8700017A ES2005545A6 ES 2005545 A6 ES2005545 A6 ES 2005545A6 ES 8700017 A ES8700017 A ES 8700017A ES 8700017 A ES8700017 A ES 8700017A ES 2005545 A6 ES2005545 A6 ES 2005545A6
Authority
ES
Spain
Prior art keywords
amino
methyl
alkaline phosphatase
propanol buffer
substrate formulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8700017A
Other languages
English (en)
Inventor
Jeffrey W Steaffens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of ES2005545A6 publication Critical patent/ES2005545A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/50—Indoles
    • C12Q2334/52—5-Bromo-4-chloro-3-indolyl, i.e. BCI
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/967—Standards, controls, materials, e.g. validation studies, buffer systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

COMPRENDE: A) MEZCLAR UNA SAL DE TETRAZOLIO CON AGUA Y AGITAR EN LA OSCURIDAD DURANTE 30 MINUTOS APROXIMADAMENTE PARA OBTENER UNA SOLUCION A; B) AGREGAR 2-AMINO-2-METIL-1-PROPANOL EN AGUA AGITANDO Y MEZCLAR DURANTE UNOS 5 MINUTOS POR LO MENOS, INCORPORAR UN CONGENERE INDIGO FOSFATADO Y SEGUIR AGITANDO CON POCA LUZ DURANTE UNOS 20 MINUTOS COMO MINIMO Y AJUSTAR EL PH A 9,7-9,9 PARA OBTENER UNA SOLUCION B; C) MEZCLAR LENTAMENTE LAS SOLUCIONES A Y B CON AGITACION Y OPCIONALMENTE AÑADIR UNA SOLUCION DE MGC12 Y/O AZIDA SODICA Y CONTINUAR LA AGITACION HASTA DISOLUCION COMPLETA DE TODOS LOS SOLIDOS.ESTA FORMULACION SE UTILIZA EN LOS ENSAYOS DE FOSFATASA ALCALINA.
ES8700017A 1986-01-08 1987-01-05 Un procedimiento para la preparacion de una formulacion de sustrato para un ensayo de fosfatasa alcalina. Expired ES2005545A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/817,090 US4748115A (en) 1986-01-08 1986-01-08 Substrate formulation in 2-amino-2-methyl-1-propanol buffer for alkaline phosphatase assays

Publications (1)

Publication Number Publication Date
ES2005545A6 true ES2005545A6 (es) 1989-03-16

Family

ID=25222331

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8700017A Expired ES2005545A6 (es) 1986-01-08 1987-01-05 Un procedimiento para la preparacion de una formulacion de sustrato para un ensayo de fosfatasa alcalina.

Country Status (8)

Country Link
US (1) US4748115A (es)
EP (1) EP0228663B1 (es)
JP (1) JPH0695958B2 (es)
AT (1) ATE79411T1 (es)
AU (1) AU595570B2 (es)
CA (1) CA1293677C (es)
DE (1) DE3686409T2 (es)
ES (1) ES2005545A6 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5443958A (en) * 1987-08-14 1995-08-22 Director-General, National Institute Of Animal Industry 2-acrylamine-2-methyl-1-propanesulfonic acid enhancement of alkaline phosphatase label detection
US4956302A (en) * 1987-09-11 1990-09-11 Abbott Laboratories Lateral flow chromatographic binding assay device
US4892817A (en) * 1987-09-21 1990-01-09 Biogenex Laboratories Stable phosphatase substrate composition
IL85018A0 (en) * 1988-01-03 1988-06-30 Orgenics Ltd Stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt,method of making and using same in biological and diagnostic assays
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
US5137804A (en) * 1988-05-10 1992-08-11 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5208143A (en) * 1988-11-17 1993-05-04 Becton, Dickinson And Company Immunoassay on a preblocked solid surface
US5135847A (en) * 1988-11-18 1992-08-04 Becton, Dickinson And Company Substrate composition for alkaline phosphatase and method for assay using same
CA2023850A1 (en) * 1989-09-28 1991-03-29 Randal A. Hoke Stabilizing reagent for membrane immunoassay
KR910014706A (ko) * 1990-01-10 1991-08-31 원본미기재 세척이 필요없는 개량된 이뮤노어세이(immunoassay)장치
WO1994001544A1 (en) * 1992-07-02 1994-01-20 Boehringer Mannheim Corporation A reagent stabilized by bivalent metal salts
EP0648278B1 (en) * 1992-07-02 1999-12-08 Roche Diagnostics Corporation Stabilizing tetrazolium salts in a reagent
DE19639538A1 (de) * 1996-09-26 1998-04-09 Boehringer Mannheim Gmbh Enzymatisches Verfahren zum Nachweis der Erhitzungsbehandlung von Milch
ES2143934B1 (es) * 1998-01-23 2000-12-16 Univ Oviedo Cuantificacion amperometrica y voltamperometrica de azul de indigo para la determinacion de hidrolasas y desarrollo de biosensores.
DE19830478A1 (de) * 1998-07-08 2000-01-13 Friedrich Schiller Uni Jena Bu Verfahren zum Nachweis von alkalischer Phosphatase und Phosphatasekonjugaten
US6099892A (en) * 1998-10-01 2000-08-08 Pumpkin Ltd. Protective coating for decorative vegetable material
DE60224839T2 (de) * 2001-09-28 2009-01-29 Arkray, Inc. Verfahren zur lagerung von tetrazoliumverbindungen, dafür bestimmter stabilisator und nach dem verfahren aufbewahrte tetrazoliumverbindungsreagenzlösung
JP3910174B2 (ja) * 2001-10-11 2007-04-25 アークレイ株式会社 アジ化ナトリウムを用いた測定方法
WO2019139980A1 (en) * 2018-01-09 2019-07-18 Idexx Laboratories, Inc. Methods for measuring analyte and/or protein in biological samples

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4030995A (en) * 1976-07-13 1977-06-21 Sigma Chemical Company Alkaline phosphatase isoenzyme determination
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes

Also Published As

Publication number Publication date
DE3686409D1 (de) 1992-09-17
AU6713387A (en) 1987-07-09
ATE79411T1 (de) 1992-08-15
CA1293677C (en) 1991-12-31
US4748115A (en) 1988-05-31
EP0228663B1 (en) 1992-08-12
JPH0695958B2 (ja) 1994-11-30
EP0228663A3 (en) 1988-06-01
AU595570B2 (en) 1990-04-05
JPS62166899A (ja) 1987-07-23
EP0228663A2 (en) 1987-07-15
DE3686409T2 (de) 1993-02-18

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20041004