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EP4655383A1 - Normalisation de l'ensemencement de bioréacteurs - Google Patents

Normalisation de l'ensemencement de bioréacteurs

Info

Publication number
EP4655383A1
EP4655383A1 EP24708295.1A EP24708295A EP4655383A1 EP 4655383 A1 EP4655383 A1 EP 4655383A1 EP 24708295 A EP24708295 A EP 24708295A EP 4655383 A1 EP4655383 A1 EP 4655383A1
Authority
EP
European Patent Office
Prior art keywords
cell
cells
bioreactor
volume
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24708295.1A
Other languages
German (de)
English (en)
Inventor
Abraham J. OLSON
Alexis MESA
Yingjian YOU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of EP4655383A1 publication Critical patent/EP4655383A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé d'automatisation du démarrage d'un bioréacteur et de normalisation de l'ensemencement de cultures cellulaires par comptage d'un certain nombre de cellules viables dans un échantillon cellulaire par un module de santé cellulaire. Une densité de départ souhaitée de cellules est reçue et un volume de milieu et un volume de l'échantillon cellulaire sont chacun déterminés, sur la base du nombre de cellules dans l'échantillon de cellules, pour être combinés afin d'obtenir une densité prédéterminée de cellules dans un bioréacteur. Le milieu et l'échantillon cellulaire sont combinés pour obtenir une culture cellulaire en fournissant le volume de milieu au bioréacteur et en fournissant le volume de l'échantillon cellulaire au bioréacteur.
EP24708295.1A 2023-01-25 2024-01-23 Normalisation de l'ensemencement de bioréacteurs Pending EP4655383A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363441094P 2023-01-25 2023-01-25
PCT/US2024/012621 WO2024158817A1 (fr) 2023-01-25 2024-01-23 Normalisation de l'ensemencement de bioréacteurs

Publications (1)

Publication Number Publication Date
EP4655383A1 true EP4655383A1 (fr) 2025-12-03

Family

ID=90097628

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24708295.1A Pending EP4655383A1 (fr) 2023-01-25 2024-01-23 Normalisation de l'ensemencement de bioréacteurs

Country Status (4)

Country Link
EP (1) EP4655383A1 (fr)
KR (1) KR20250135878A (fr)
CN (1) CN120500526A (fr)
WO (1) WO2024158817A1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112585257A (zh) * 2018-06-19 2021-03-30 加拿大干细胞科技公司 用于细胞自动培养的系统、方法及装置
EP3699261A1 (fr) * 2019-02-20 2020-08-26 Sartorius Stedim Biotech GmbH Ensemble de dispositif de bioprocessus et procédé d'inoculation
CN114207136A (zh) * 2019-06-07 2022-03-18 朱诺治疗学股份有限公司 自动化t细胞培养

Also Published As

Publication number Publication date
CN120500526A (zh) 2025-08-15
KR20250135878A (ko) 2025-09-15
WO2024158817A1 (fr) 2024-08-02

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