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EP1699560A1 - Pointe de pipette, systeme de pipette, dispositif d'actionnement de pipette et procede de pipettage dans la plage du nanolitre - Google Patents

Pointe de pipette, systeme de pipette, dispositif d'actionnement de pipette et procede de pipettage dans la plage du nanolitre

Info

Publication number
EP1699560A1
EP1699560A1 EP05823001A EP05823001A EP1699560A1 EP 1699560 A1 EP1699560 A1 EP 1699560A1 EP 05823001 A EP05823001 A EP 05823001A EP 05823001 A EP05823001 A EP 05823001A EP 1699560 A1 EP1699560 A1 EP 1699560A1
Authority
EP
European Patent Office
Prior art keywords
pipette tip
elastic tube
tube
actuator
radially elastic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP05823001A
Other languages
German (de)
English (en)
Other versions
EP1699560B1 (fr
Inventor
Peter Koltay
Wolfgang Streule
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP1699560A1 publication Critical patent/EP1699560A1/fr
Application granted granted Critical
Publication of EP1699560B1 publication Critical patent/EP1699560B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers

Definitions

  • an end of the described squish tube means spaced from the pipette tip is connectable to a syringe via a three-way valve such that sample liquid and a diluent previously drawn into the syringe are actuated by actuating the syringe and pressurizing the syringe Hole of the block can be ejected through the pipette tip into a discharge vessel.
  • a second end having a radially elastic tube with an orifice, the resilient tube being configured to be substantially rigid to deflections along the tube axis but flexible and resilient to radial deformations,
  • first end is adapted to be coupled to a mating coupling device of a pipetting device, such that liquid can be sucked through the muzzle into the fluid region by an actuating device in the pipetting device.
  • a first actuator for generating a negative pressure at the fluid port at the first end of the pipette tip to aspirate fluid through the mouth of the radially elastic tube; and a second actuator for temporally varying the volume of a portion of the radially elastic tube to thereby expel liquid as a droplet or as a free-flying jet from the mouth of the radially elastic tube.
  • an actuator for temporally varying the volume of a portion of the radially elastic tube to thereby expel liquid as a droplet or as a free-flying jet from the mouth of the radially elastic tube when the fixing device is in the first state.
  • a radially elastic tube is to be understood as one which is flexible and elastic with respect to radial deformations.
  • the radially elastic tube may be formed by an elastic tube, i. H. a fluid conduit which also has deflections along the tube axis, i. H. in the axial direction, can be flexible.
  • an elastic tube i. H. a fluid conduit which also has deflections along the tube axis, i. H. in the axial direction
  • elastic hoses tend to behave more like a tube, in the case of lengths typically used in a microdosing device, i. H. substantially rigid with respect to deflections along the tube axis, but flexible and elastic with respect to radial deformations.
  • Typical materials for the radially elastic tube include the polyimide, polyamide or silicone. Typical diameters can be from 0.1 to 1 mm.
  • the tube, which is elastic in the radial direction can be attached to the pipette tip body, for example by gluing, injecting, shrinking or by press-fitting. be satisfied.
  • the elastic in the radial direction tube may further arbitrarily shaped, for. B. have round or angular, constant or changing cross sections.
  • the present invention includes devices and a method that can be integrated into conventional automatic pipetting machines and provide the ability to deliver volumes in the range of 0.1 nL to several ⁇ L.
  • the present invention also allows for intermediate storage of the fluid in the pipette tip.
  • a composite of a rigid part which may have the structure of a conventional pipette tip, and an elastic tube is used according to the invention.
  • the rigid part allows the picking up of the pipette tip in the automatic pipetting machines and allows the ejection by means of conventional devices built into the machine.
  • the rigid part may allow for inclusion in a handheld pipette.
  • the tube attached to the underside of the pipette serves to deliver the fluid in the pipette tip by the method as described above with reference to Figs. 7a to 7c and as set forth in German application 10337484.
  • the dosing volume dispensed without contact may be in the range from 0.1 nL to 10OnL per individual dosing operation. Multiple dosing allows a higher dosing volume to be achieved with flow rates of up to 20 ⁇ L / s.
  • the present invention also allows the use of different drive units for aspiration and dosing.
  • the aspirating can be done, for example, by a conventional automatic pipetting device which generates a vacuum at the first end of the pipette tip.
  • aspiration can also be driven only by capillary forces.
  • a capillary-driven aspiration is described, for example, in GB 2353093A.
  • the dispensing can be done by an actuator for generating a volume change of a tube elastic in the radial direction, or by overpressure or inertial forces, such as.
  • GB 2353093 A or DE 19913076 A be brought about.
  • Fig. 5 schematically shows a conventional hand pipette
  • FIG. 6 shows schematically a movable holder of a conventional automatic pipetting device
  • Fig. 7a to 7c are schematic representations for explaining a preferred dosing method used according to the invention.
  • the pipette tip 100 includes a rigid pipette tip body 101 and an elastic tube 102 attached to a portion 104 on the pipette tip body 101.
  • the pipette tip 100 includes a first end 106 formed by the rigid pipette tip body 101 and adapted to be removably attached to a pipetting machine or a hand-held pipette. te to be attached.
  • the first end 106 includes a fluid port 106a fluidly connected to a fluid chamber 108 in the micropipette.
  • the interior of the tube 102 is also fluidly connected to the fluid chamber 108.
  • the elastic tube is disposed at a second end 110 of the pipette tip and has a mouth or fluid opening 112 through which liquid can enter and exit.
  • a controller 130 is provided and connected to the positioning means 126 and the pressure generating means 128 to control the operation thereof.
  • the pipetting device has, in addition to the pressure-generating device, an actuating device or actuator.
  • Drive means 132 to eject liquid from the elastic tube of the pipette tip 100.
  • the controller 130 is also connected to the actuator 132 to control the operation thereof.
  • a complete pipetting cycle using a pipetting device as described above with reference to FIG. 2, for example, may look like this.
  • the pipette tip 100 is received from a carrier (carrier) using the pipetting unit 120 and in particular the coupling device 122.
  • a recording can proceed in accordance with conventional recording methods provided by conventional pipetting automata.
  • the pipetting unit 120 is moved by use of the positioning device 126 to immerse the elastic tube of the pipette tip in the liquid.
  • a negative pressure which is provided by the pressure generating device 128 as in conventional automatic pipetting, the liquid is drawn through the tube into the pipette tip.
  • the dosing unit is moved together with the pipette tip by the positioning device 126 to the delivery point, d. H .
  • the hose and actuating device are preferably designed to discharge a metering of liquid volumes in the nanoliter range, for example between 0.1 and 100 nL per metering operation. In this case, several dosing operations to different destinations or. take place in different vessels from the same pipette tip.
  • a volume of liquid possibly remaining in the pipette tip can be ejected in pressure-driven manner by air-cushion pipettes in order to completely empty the pipette tip and, if necessary, recover valuable liquid.
  • the metering unit can be moved using the positioning device 126 to a storage container where the pipette tip can be detached from the pipetting unit and coupler 122 and into the storage container using an ejection mechanism that may correspond to that of conventional automatic pipetting machines is given.
  • the positioning device may suitably include drive mechanisms, which may include motors and gears, to effectuate the desired movements of the dosing unit 120.
  • the controller 130 may in any suitable manner, for example by using a microprocessor, be adapted to the positioning means 126 to control the pressure-generating device 128 and the 'actuator 132nd An exemplary embodiment of the actuator 132 will be described below with reference to FIGS. 3 and 4 explained in more detail.
  • FIGS. 3a and 3b For the sake of clarity, only the pipette tip 100 and the sections of the actuating device 132 required for explanation are shown in FIGS. 3a and 3b. Furthermore, in FIGS. 3a and 3b, respective elements are shown at least partially transparent in order to simplify the explanation.
  • the actuating device comprises a first clamping jaw 134, a second clamping jaw 136 and an actuating member 138.
  • the clamping jaws 134 and 136 are mounted via slide rails 140, 142, wherein a suitable drive mechanism 143 (shown schematically in FIG. 4b) to move the jaws 134, 136 relative to one another along the rails 140, 142.
  • a suitable drive mechanism 144 ( Figure 4b) is also provided by which the actuator 138 can be moved substantially parallel to the tracks 140 and 142.
  • Figures 4a to 4c illustrate cross-sectional views with a section axis passing centrally through the recesses 150 in the jaws 134 and 136.
  • Fig. Figure 4b shows the actuator after the jaws 134 and 136 are automatically closed.
  • the actuator 138 is driven up by the drive mechanism 144 through the opening of the jaw 136 provided therefor to reduce the volume of the elastic tube 102 thereby To eject liquid as a free-flowing droplet or as a free-flowing jet from the mouth 112 of the elastic tube 102.
  • the jaw 134 acts as a counter holding element.
  • the phase in which the volume of the tube is reduced is shown in Fig. 4c.
  • the jaw 136 and actuator 138 are preferably moved together, whereupon the actuator is actuated to the position shown in FIG. 4c.
  • FIGS. 3a to 4c are purely schematic in this respect, wherein the actuating member and the counter-holding element may be shaped in a suitable manner so as to cause only a partial squeezing of the sleeve. ches or even a complete squeezing of the hose to allow.
  • the stroke of the drive means 144 of the actuator 138 may be adjustable so that by adjusting the stroke, different drop volumes may be expelled from the mouth 112 of the flexible tube 102.
  • the ejection method reference is again made to the above description of FIGS. 7a to 1c and also to the German application 10337484.1.
  • a centering auxiliary device 145 is provided to center the pipette tip in the actuator 132.
  • a centering aid is optional, for example if a sufficiently accurate positioning device is provided for the pipetting unit.
  • the jaws may be closed using an electromagnetic drive to fix the flexible hose.
  • the actuator may be electromagnetically or piezoelectrically driven to provide the desired nanoliter dosage.
  • multiple dosing may optionally be done to different targets or into different vessels. After the dosing process, the jaws can be opened and the pipette tip can be removed by a conventional automatic pipetting machine with a corresponding mechanism.
  • the pipette tips may be dispensed in a waste box or in a storage carrier by means of an ejector mechanism (not shown) located in the pipetting machine.
  • an ejector mechanism located in the pipetting machine.
  • the dosing cycle can be resumed with the movement of the pipette tip to the dispensing position later.
  • the pipette tip can also be deposited in a bearing carrier immediately after aspiration and can only be removed again at a later time in order to carry out a dispensing of a volume of the aspirated liquid.
  • the nozzle opening has no contact whatsoever with the actuating device (drive unit) so that carry-over of the liquid is prevented.
  • the pipette tips may also be dispensed by the pipetting unit (eg, 120 in Fig. 2) to the actuator that includes the fixation device so that fluid delivery may occur at a location other than the receptacle, with the pipette tip not communicating with the pipette unit for fluid delivery or the automatic vending machine must be coupled.
  • the automatic petting machine or the pipetting unit can be used elsewhere during dosing by the actuating device for ejecting a liquid from the hose.
  • the present invention provides a novel pipetting device or components for a pipetting device.
  • a conventional Pipetierautomat be used together with inventive pipette tips and an actuator according to the invention for such pipette tips.
  • the pipette tips according to the invention without the actuating device according to the invention.
  • the dispensing can by conventional methods, such.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne une pointe de pipette (100) remplaçable, qui comprend une première extrémité (106) munie d'une ouverture pour fluide (106a) et d'une seconde extrémité (110) qui présente un tube (102) élastique dans le sens radial. La bouche (112) et l'ouverture pour fluide (106a) sont en communication fluidique. La première extrémité (106) est conçue de manière à pouvoir être raccordée à un dispositif d'accouplement (122) d'un système de pipette (120), de sorte que du liquide puisse être aspiré dans la zone de fluide (108), par un dispositif d'actionnement, dans le système de pipette (120). Un changement de volume d'une section du tube élastique dans le sens radial permet d'éjecter le liquide sous forme de gouttelettes libres ou de faisceau, hors de la bouche du tube élastique dans le sens radial.
EP05823001A 2005-01-19 2005-12-19 Pointe de pipette, systeme de pipette, dispositif d'actionnement de pipette et procede de pipettage dans la plage du nanolitre Expired - Lifetime EP1699560B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005002525A DE102005002525A1 (de) 2005-01-19 2005-01-19 Pipettenspitze, Pipetiervorrichtung, Pipettenspitzen-Betätigungsvorrichtung und Verfahren zum Pipetieren im nL-Bereich
PCT/EP2005/013671 WO2006076957A1 (fr) 2005-01-19 2005-12-19 Pointe de pipette, systeme de pipette, dispositif d'actionnement de pipette et procede de pipettage dans la plage du nanolitre

Publications (2)

Publication Number Publication Date
EP1699560A1 true EP1699560A1 (fr) 2006-09-13
EP1699560B1 EP1699560B1 (fr) 2007-08-22

Family

ID=35744657

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05823001A Expired - Lifetime EP1699560B1 (fr) 2005-01-19 2005-12-19 Pointe de pipette, systeme de pipette, dispositif d'actionnement de pipette et procede de pipettage dans la plage du nanolitre

Country Status (5)

Country Link
US (1) US8071049B2 (fr)
EP (1) EP1699560B1 (fr)
AT (1) ATE370788T1 (fr)
DE (2) DE102005002525A1 (fr)
WO (1) WO2006076957A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9067051B2 (en) 2010-06-02 2015-06-30 Technische Universitat Berlin Valve device for controlling a flow of a fluid through a fluid channel, arrangement and multi-way valve device

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ES2424216T3 (es) 2010-06-02 2013-09-30 Technische Universität Berlin Dispositivo de válvula para controlar un flujo de un fluido a través de un canal de fluido, disposición así como dispositivo de múltiples vías
USD698458S1 (en) * 2011-08-18 2014-01-28 Bioptic, Inc. Disposable bio-analysis cartridge
EP2662137A1 (fr) 2012-05-08 2013-11-13 Roche Diagniostics GmbH Ensemble de distribution
DE102012209314B4 (de) 2012-06-01 2015-04-02 Albert-Ludwigs-Universität Freiburg Vorrichtung und Verfahren zur Abgabe oder Aufnahme eines Flüssigkeitsvolumens
DE202013003390U1 (de) 2013-04-11 2014-07-14 Brand Gmbh + Co Kg Pipettiervorrichtung mit einer Mikrodosiereinheit
DE102013006227A1 (de) 2013-04-11 2014-10-16 Brand Gmbh + Co Kg Pipettiervorrichtung mit einer Mikrodosiereinheit
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DE202016004140U1 (de) 2016-07-05 2017-10-08 Brand Gmbh + Co Kg Pipettiervorrichtung zum Aspirieren und Dispensieren von Flüssigkeiten
WO2019083512A1 (fr) * 2017-10-24 2019-05-02 Hewlett-Packard Development Company, L.P. Distributeur de fluide
EP3489693B1 (fr) 2017-11-22 2020-09-09 Brand Gmbh + Co Kg Procédé de commande d'un dispositif de pipetage
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US10625254B2 (en) 2017-11-22 2020-04-21 Brand Gmbh + Co Kg Method for controlling a pipetting device
DE102018131088A1 (de) * 2018-12-05 2020-06-10 Biofluidix Gmbh Flüssigkeitsdosiervorrichtung zur ballistischen Abgabe von Dosiermengen im Nanoliterbereich, Flüssigkeitsdosierverfahren und Pipettierspitze hierfür
DE102019113679A1 (de) * 2019-05-22 2020-11-26 Hamilton Storage Gmbh Flüssigkeit-Screeningbaugruppe mit mechanischer Auslösung kleinster Flüssigkeitsmengen
CN115106143A (zh) * 2022-06-29 2022-09-27 合肥瀚海星点生物科技有限公司 一种高精度电动微量液体移液器
DE102022211313A1 (de) * 2022-10-25 2024-04-25 Biofluidix Gmbh Dosiermodul mit Betätigungsfenster
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Also Published As

Publication number Publication date
DE102005002525A1 (de) 2006-07-27
US8071049B2 (en) 2011-12-06
EP1699560B1 (fr) 2007-08-22
US20060171854A1 (en) 2006-08-03
ATE370788T1 (de) 2007-09-15
DE502005001306D1 (de) 2007-10-04
WO2006076957A1 (fr) 2006-07-27

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