EP1497428A4 - SURFACE TRANSFECTION AND EXPRESSION PROCESS - Google Patents

SURFACE TRANSFECTION AND EXPRESSION PROCESS

Info

Publication number: EP1497428A4
Authority: EP; European Patent Office
Prior art keywords: expression process; surface transfection; transfection; expression
Prior art date: 2002-04-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP03747024A

Other languages

German (de)

French (fr)

Other versions

EP1497428A2 (en

Inventor

Michael D Uhler

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of Michigan System

University of Michigan Ann Arbor

Original Assignee

University of Michigan System

University of Michigan Ann Arbor

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2002-04-16

Filing date

2003-04-16

Publication date

2006-01-18

2003-04-16 Application filed by University of Michigan System, University of Michigan Ann Arbor filed Critical University of Michigan System

2005-01-19 Publication of EP1497428A2 publication Critical patent/EP1497428A2/en

2006-01-18 Publication of EP1497428A4 publication Critical patent/EP1497428A4/en

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
- B01J2219/00315—Microtiter plates
- B01J2219/00317—Microwell devices, i.e. having large numbers of wells
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/0061—The surface being organic
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/00626—Covalent
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/0074—Biological products
- B01J2219/00743—Cells
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

EP03747024A 2002-04-16 2003-04-16 SURFACE TRANSFECTION AND EXPRESSION PROCESS Withdrawn EP1497428A4 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US10/123,435 US6902933B2 (en)	2000-11-03	2002-04-16	Surface transfection and expression procedure
US123435		2002-04-16
PCT/US2003/011921 WO2003089600A2 (en)	2002-04-16	2003-04-16	Surface transfection and expression procedure

Publications (2)

Publication Number	Publication Date
EP1497428A2 EP1497428A2 (en)	2005-01-19
EP1497428A4 true EP1497428A4 (en)	2006-01-18

Family

ID=29248344

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP03747024A Withdrawn EP1497428A4 (en)	2002-04-16	2003-04-16	SURFACE TRANSFECTION AND EXPRESSION PROCESS

Country Status (6)

Country	Link
US (2)	US6902933B2 (en)
EP (1)	EP1497428A4 (en)
AU (1)	AU2003226419B2 (en)
CA (1)	CA2481986A1 (en)
IL (1)	IL164535A0 (en)
WO (1)	WO2003089600A2 (en)

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US7351578B2 (en)	1999-12-10	2008-04-01	Invitrogen Corp.	Use of multiple recombination sites with unique specificity in recombinational cloning
US7198924B2 (en)	2000-12-11	2007-04-03	Invitrogen Corporation	Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6890556B1 (en)	2001-02-14	2005-05-10	Northwestern University	Controlled surface-associated delivery of genes and oligonucleotides
US6905878B2 (en) *	2002-12-19	2005-06-14	The Scripps Research Institute	DNA array for high throughput solid-phase transfection and method for producing the same
WO2005054438A2 (en)	2003-12-01	2005-06-16	Invitrogen Corporation	Nucleic acid molecules containing recombination sites and methods of using the same
US20060257858A1 (en) *	2005-04-01	2006-11-16	Invitrogen Corporation	Methods and compositions for introducing biopolymers into cells
KR101227485B1 (en) *	2005-11-25	2013-01-29	엘지전자 주식회사	Recording mdium, Method and Apparatus for recording defect management information on the recording medium
EP2765983A1 (en) *	2011-10-11	2014-08-20	Hans Kosak	Composition for introducing nucleic acids into cells
HUE059435T2 (en) *	2012-10-05	2022-11-28	Biontech Delivery Tech Gmbh	Hydroxylated polyamine derivatives as transfection reagents
CN112370532B (en) *	2012-10-08	2024-11-01	生物技术传送科技有限责任公司	Carboxylated polyamine derivatives as transfection reagents
US20170191080A1 (en) *	2013-07-15	2017-07-06	Baden-Württemberg Stiftung Ggmbh	Solid Phase Transfection of Proteins and Nucleic Acids
US10294454B2 (en)	2016-08-24	2019-05-21	General Electric Company	Methods and kits for cell activation

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DE4104186A1 (en) *	1991-02-12	1992-08-13	Genentech Inc	NEW COMPLEXES INCLUDING ENDOCYTOSIS IN HIGHER EUKARYOTIC CELLS, NUCLEIC ACID
US5569588A (en) *	1995-08-09	1996-10-29	The Regents Of The University Of California	Methods for drug screening
WO1998040502A1 (en) *	1997-03-14	1998-09-17	Life Technologies, Inc.	Peptide-enhanced transfections
US20020006664A1 (en) *	1999-09-17	2002-01-17	Sabatini David M.	Arrayed transfection method and uses related thereto

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US4683202A (en)	1985-03-28	1987-07-28	Cetus Corporation	Process for amplifying nucleic acid sequences
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JP4338106B2 (en) *	1995-06-07	2009-10-07	ライフテクノロジーズコーポレーション	Peptide enhanced cationic lipid transfection
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2002
- 2002-04-16 US US10/123,435 patent/US6902933B2/en not_active Expired - Fee Related
2003
- 2003-04-16 AU AU2003226419A patent/AU2003226419B2/en not_active Ceased
- 2003-04-16 EP EP03747024A patent/EP1497428A4/en not_active Withdrawn
- 2003-04-16 CA CA002481986A patent/CA2481986A1/en not_active Abandoned
- 2003-04-16 WO PCT/US2003/011921 patent/WO2003089600A2/en not_active Application Discontinuation
2004
- 2004-10-12 IL IL16453504A patent/IL164535A0/en unknown
2005
- 2005-03-18 US US11/084,411 patent/US20060078996A1/en not_active Abandoned

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Also Published As

Publication number	Publication date
AU2003226419B2 (en)	2007-08-23
WO2003089600A2 (en)	2003-10-30
WO2003089600A3 (en)	2004-07-08
IL164535A0 (en)	2005-12-18
US6902933B2 (en)	2005-06-07
AU2003226419A1 (en)	2003-11-03
US20030077827A1 (en)	2003-04-24
US20060078996A1 (en)	2006-04-13
EP1497428A2 (en)	2005-01-19
CA2481986A1 (en)	2003-10-30

Publication	Publication Date	Title
DE60315460D1 (en)	2007-09-20	INTRODUCTION SLEEVE
BRPI0313238A2 (en)	2016-06-21	method
DE60319810D1 (en)	2008-04-30	ANSCHWEMMFILTERMEDIEN AND MANUFACTURING AND USE PROCESS
EP1532178A4 (en)	2006-10-25	ARTIFICIAL TRANSCRIPTION FACTORS
NO20051285L (en)	2005-04-19	Bronnkule-fall apparatus
FI20020757A0 (en)	2002-04-19	Injection procedure and apparatus
FI20020479A7 (en)	2003-09-15	Surface treatment process
FI20030893L (en)	2004-12-14	Keyboard and method for making the same
SE0202107L (en)	2004-02-26	Surface modified stainless steel
DE10393257D2 (en)	2006-01-19	Tubulysin biosynthesis genes
FI20020817A7 (en)	2003-09-15	Surface treatment process
EP1497428A4 (en)	2006-01-18	SURFACE TRANSFECTION AND EXPRESSION PROCESS
DE60322421D1 (en)	2008-09-04	IMPROVED GOGGLES
DE60334272D1 (en)	2010-11-04	heater
ATE442835T1 (en)	2009-10-15	SURFACE TREATMENT
DE50206487D1 (en)	2006-05-24	heater
ITMI20030178A1 (en)	2003-08-05	APPARATUS AND FASTENING / RETAINING PROCEDURE
NO20023358D0 (en)	2002-07-11	Method
FI20020500A7 (en)	2003-09-19	Flotation apparatus and process
DE60336104D1 (en)	2011-04-07	Display surface and manufacturing process
FI20035154A7 (en)	2003-09-15	Surface treatment process
SE0201844D0 (en)	2002-06-17	New methods and use
NO20043433L (en)	2004-08-17	Approach
ITMI20022446A1 (en)	2004-05-20	ANTI-ANDROGENETIC SUBSTANCES AND TREATMENT METHODS
SE0202568D0 (en)	2002-08-29	New process

Legal Events

Date	Code	Title	Description
2004-12-03	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2005-01-19	17P	Request for examination filed	Effective date: 20041109
2005-01-19	AK	Designated contracting states	Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
2005-01-19	AX	Request for extension of the european patent	Extension state: AL LT LV MK
2006-01-18	A4	Supplementary search report drawn up and despatched	Effective date: 20051201
2006-01-18	RIC1	Information provided on ipc code assigned before grant	Ipc: C12Q 1/68 19800101ALI20051125BHEP Ipc: C12N 15/63 19900101ALI20051125BHEP Ipc: C12N 15/87 19900101AFI20051125BHEP
2006-12-06	17Q	First examination report despatched	Effective date: 20060313
2007-07-18	17Q	First examination report despatched	Effective date: 20060313
2010-04-16	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2010-05-19	18D	Application deemed to be withdrawn	Effective date: 20091127