Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
  • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
  • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
  • G01N21/64—Fluorescence; Phosphorescence
  • G01N21/645—Specially adapted constructive features of fluorimeters
  • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
  • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
  • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
  • G01N21/64—Fluorescence; Phosphorescence
  • G01N21/645—Specially adapted constructive features of fluorimeters
  • G01N2021/6463—Optics
  • G01N2021/6473—In-line geometry
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2201/00—Features of devices classified in G01N21/00
  • G01N2201/04—Batch operation; multisample devices
  • G01N2201/0446—Multicell plate, sequential

Definitions

• the subject of the present invention is an apparatus for the measurement of fluorescence out of a liquid sample, the said apparatus comprising a source of light, from which the light of measurement is arranged so as to be passed vertically through optics, a monochromator, and through the sample placed in a pit plate to a measurement monochromator and a detector.
• the samples have been dosed into small cuvettes, which may also be arranged in the form of a line or plate-
• the measurement takes place in the longitudinal axial direction of the cuvette so that the cuvette is placed in the apparatus in the space between the source of light required and the detector.
• a monochromatic excitation light and a detector measuring the fluorescence radi ⁇ ation emitted from the sample are required.
• a monochromatic excitation light and a detector measuring the fluorescence radi ⁇ ation emitted from the sample are required.
• a monochromator such as an interference filter or a grid. Since fluorescence radiation is emitted uniformly in all directions, in principle it can be measured from any direction whatsoever. A factor disturbing the measurement is the strong excitation light, wherefore attempts have been made to minimize its effect on the measurement result by means of various arrangements.
• the commonest mode of measurement in use is the so-called 90° measurement geometry.
• the directions of the excitation light and of the measure- ment are at an angle of 90 in relation to each other. In this way the access of direct excitation light to the detector is eliminated.
• the procedure in itself is simple, but it imposes considerable limitations on the geometry of the object to be measured.
• the sample cuvettes can be treated only one by one or in the line form.
• It is also possible to measure the fluorescence radiation emitted by the surface by means of a particular arrangement. In this case, the direc ⁇ tions of excitation and measurement form a rather small ( ⁇ 10 to 20°) angle.
• Such a measurement geometry is usually used when the sample absorbs either the exci- tation radiation or the emitted fluorescence radiation strongly.
• the apparatus for the measurement of fluore ⁇ scence in accordance with the invention is mainly char ⁇ acterized in that, for each process of measurement, the source of light, the optics, the monochromator, the sample to be measured, the measurement monochromator, and the detector are arranged so that they can be brought onto the same vertical straight line in relation to each other, in this sequence.
• the invention comes out more closely from the following description and from the attached draw ⁇ ings, wherein
• Figure 1 is a schematical side view of the apparatus for the measurement of fluorescence and Figure 2 shows a section at A-A in Fig. 1.
• the source 1 of light and the detector 6 are placed at opposite sides of the sample to be measured (Fig. 1) .
• the monochromator 5 in front of the detector 6 has been selected so that it is penetrable by the desired measurement wave-length ⁇ i only, but not by the excitation radiation ⁇ o producing the fluorescence.
• Such a measurement geometry is highly advantageous when such samples are. supposed to be measured as are dosed in a so-called pit plate 4.
• the most important requirement regarding the source 1 of light is a sufficient light intensity at the wave-length ⁇ o. Suitable sources of light are xenon or mercury gas-discharge lamps. Laser may also be used in some applications. In such a case, no monochromator 3 is required. As a detector 6, it is favourable to use a photomultiplier tube. As monochromators 3 and 5, it is possible to use interference filters or grids. The monochromator 3 and/or the measurement monochromator 5 are preferably exchangeable or removable out of the line of measurement.

Landscapes

• Health & Medical Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Physics & Mathematics (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Immunology (AREA)
• Pathology (AREA)
• Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

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• 1981
  • 1981-09-21 FI FI812933A patent/FI812933L/fi not_active Application Discontinuation
• 1982
  • 1982-09-20 EP EP82902816A patent/EP0088781A1/en not_active Withdrawn
  • 1982-09-20 JP JP57502814A patent/JPS58501735A/ja active Pending
  • 1982-09-20 WO PCT/FI1982/000036 patent/WO1983001111A1/en not_active Ceased

Non-Patent Citations (1)

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