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EA201990035A1 - Генно-инженерные бета-глюкозидазы и способы глюкозилирования - Google Patents

Генно-инженерные бета-глюкозидазы и способы глюкозилирования

Info

Publication number
EA201990035A1
EA201990035A1 EA201990035A EA201990035A EA201990035A1 EA 201990035 A1 EA201990035 A1 EA 201990035A1 EA 201990035 A EA201990035 A EA 201990035A EA 201990035 A EA201990035 A EA 201990035A EA 201990035 A1 EA201990035 A1 EA 201990035A1
Authority
EA
Eurasian Patent Office
Prior art keywords
enzymes
glucosidases
genetic
beta
engineering
Prior art date
Application number
EA201990035A
Other languages
English (en)
Inventor
Джек Лян
Джонатан Врум
Стефани Сью Галани
Никки Деллас
Рави Давид Гарсия
Original Assignee
Кодексис, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Кодексис, Инк. filed Critical Кодексис, Инк.
Publication of EA201990035A1 publication Critical patent/EA201990035A1/ru

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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01169—Protein O-GlcNAcase (3.2.1.169)

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к генно-инженерным β-глюкозидазным (BGL) ферментам, полипептидам, обладающим BGL-активностью, и полинуклеотидам, кодирующим эти ферменты, а также к векторам и клеткам-хозяевам, содержащим эти полинуклеотиды и полипептиды. Настоящее изобретение также относится к композициям, содержащим данные ферменты, и к способам применения генно-инженерных BGL-ферментов для получения продуктов с β-глюкозными связями.
EA201990035A 2016-06-15 2017-06-09 Генно-инженерные бета-глюкозидазы и способы глюкозилирования EA201990035A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662350625P 2016-06-15 2016-06-15
PCT/US2017/036704 WO2017218325A1 (en) 2016-06-15 2017-06-09 Engineered beta-glucosidases and glucosylation methods

Publications (1)

Publication Number Publication Date
EA201990035A1 true EA201990035A1 (ru) 2019-06-28

Family

ID=60663309

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201990035A EA201990035A1 (ru) 2016-06-15 2017-06-09 Генно-инженерные бета-глюкозидазы и способы глюкозилирования

Country Status (14)

Country Link
US (2) US11299723B2 (ru)
EP (1) EP3471744A4 (ru)
JP (1) JP2019517801A (ru)
KR (1) KR20190018512A (ru)
CN (1) CN109715182A (ru)
AU (1) AU2017284104B2 (ru)
BR (1) BR112018075932A2 (ru)
CA (1) CA3027189A1 (ru)
EA (1) EA201990035A1 (ru)
IL (1) IL263597A (ru)
MX (1) MX2018015534A (ru)
PH (1) PH12018502656A1 (ru)
SG (1) SG11201810998WA (ru)
WO (1) WO2017218325A1 (ru)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019517801A (ja) * 2016-06-15 2019-06-27 コデクシス， インコーポレイテッド 操作されたβ−グルコシダーゼおよびグルコシル化方法
CN109097423B (zh) * 2018-08-13 2021-05-28 江南大学 应用交替糖蔗糖酶催化合成单、二葡萄糖基莱鲍迪苷a
CN109355275B (zh) * 2018-11-21 2021-08-20 中山大学 高热稳定性β-葡萄糖苷酶突变体及其应用
CN110283809A (zh) * 2019-06-28 2019-09-27 苏州大学 一种β-葡糖苷酶固定化方法及应用

Family Cites Families (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4356196A (en) 1980-10-20 1982-10-26 Hultquist Joe H Process for treating alfalfa and other cellulosic agricultural crops
US5037663A (en) 1981-10-14 1991-08-06 Colorado State University Research Foundation Process for increasing the reactivity of cellulose-containing materials
US4600590A (en) 1981-10-14 1986-07-15 Colorado State University Research Foundation Method for increasing the reactivity and digestibility of cellulose with ammonia
JPS58149697A (ja) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
US4556430A (en) 1982-09-20 1985-12-03 Trustees Of Dartmouth College Process for hydrolysis of biomass
JP2815023B2 (ja) 1989-10-17 1998-10-27 農林水産省食品総合研究所長 セロビオースの製造方法
US5171592A (en) 1990-03-02 1992-12-15 Afex Corporation Biomass refining process
KR100356926B1 (ko) 1993-09-07 2003-01-10 교와 핫꼬 고교 가부시끼가이샤 트랜스-4-히드록시-l-프롤린의제조법
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
KR970703426A (ko) 1994-06-03 1997-07-03 제임스 쉐한 정제된 Myceliophthora 락카제 및 그것을 암호화 하는 핵산(PURIFIED MYCELIOPHTHORA LACCASES AND NUCLEIC ACIDS ENCODING SAME)
DE69534185T2 (de) 1994-06-30 2006-02-23 Novozymes Biotech, Inc., Davis Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
EA000169B1 (ru) 1995-03-25 1998-10-29 Рон-Пуленк Родиа Акциенгезелльшафт Способ активации полисахаридов, получаемые согласно этому способу полисахариды и их применение
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
ATE334225T1 (de) 1997-01-17 2006-08-15 Maxygen Inc Evolution prokaryotischer ganzer zellen durch rekursive sequenzrekombination
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
IL138002A0 (en) 1999-01-19 2001-10-31 Maxygen Inc Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
CA2362737A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
US7090973B1 (en) * 1999-04-09 2006-08-15 Oscient Pharmaceuticals Corporation Nucleic acid sequences relating to Bacteroides fragilis for diagnostics and therapeutics
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
US7049485B2 (en) 2000-10-20 2006-05-23 Board Of Trustees Of Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
KR100851610B1 (ko) 2001-09-21 2008-08-12 디아이씨 가부시끼가이샤 감미료 및 그 제조 방법
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
DK1493027T3 (en) 2002-03-01 2014-11-17 Codexis Mayflower Holdings Llc Methods, systems and software for identifying functional biomolecules
WO2003078583A2 (en) 2002-03-09 2003-09-25 Maxygen, Inc. Optimization of crossover points for directed evolution
WO2005017135A1 (en) 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
US20060041961A1 (en) 2004-03-25 2006-02-23 Abad Mark S Genes and uses for pant improvement
CA2603774C (en) 2005-04-12 2015-11-17 E.I. Du Pont De Nemours And Company System and process for biomass treatment
WO2006128304A1 (en) 2005-06-03 2006-12-07 Iogen Energy Corporation Method of continuous processing of lignocellulosic feedstocks
EP2118281A2 (en) 2007-02-12 2009-11-18 Codexis, Inc. Structure-activity relationships
US20080295980A1 (en) 2007-05-31 2008-12-04 Lignol Innovations Ltd. Continuous counter-current organosolv processing of lignocellulosic feedstocks
US8093028B2 (en) 2007-07-24 2012-01-10 Wisconsin Alumni Research Foundation Engineered glycosyltransferases with expanded substrate specificity
US7807419B2 (en) 2007-08-22 2010-10-05 E. I. Du Pont De Nemours And Company Process for concentrated biomass saccharification
EP2250594B1 (en) 2008-02-12 2017-04-19 Codexis, Inc. Method of generating an optimized, diverse population of variants
EP2250595B1 (en) 2008-02-12 2017-06-14 Codexis, Inc. Method of selecting an optimized diverse population of variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
EP2285958B1 (en) 2008-06-13 2016-03-09 Codexis, Inc. Method of synthesizing polynucleotide variants
US8328947B2 (en) 2008-08-29 2012-12-11 Iogen Energy Corporation Method for low water hydrolysis or pretreatment of polysaccharides in a lignocellulosic feedstock
EP2443235A4 (en) 2009-06-16 2013-07-31 Codexis Inc ß-glucosidase VARIANTS
SG179197A1 (en) 2009-09-18 2012-04-27 Codexis Inc Reduced codon mutagenesis
SI2357227T1 (sl) 2010-02-11 2015-11-30 Sued-Chemie Ip Gmbh & Co. Kg Optimizirani celulazni encimi
MX373909B (es) 2010-06-02 2020-07-09 Minnesota Specialty Yeast Llc Produccion recombinante de esteviol glucosidos.
US20130004979A1 (en) 2010-06-11 2013-01-03 Wisconsin Alumni Research Foundation Glycosyltransferase reversibility for sugar nucleotide synthesis and microscale scanning
HUE042817T2 (hu) 2011-06-28 2019-07-29 Codexis Inc Fehérjevariánsok elõállítása régiókeveréssel
WO2013133689A1 (en) 2012-03-08 2013-09-12 Purecircle Sdn Bhd High-purity rubusoside and process for producing of the same
EP3444338A1 (en) 2012-01-23 2019-02-20 DSM IP Assets B.V. Diterpene production
WO2013138339A1 (en) 2012-03-15 2013-09-19 Codexis, Inc. Gene shuffling methods
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
CN110358795B (zh) 2012-05-22 2024-12-03 谱赛科有限责任公司 高纯度的甜菊醇糖苷
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
TW201402599A (zh) 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
EP2900677A4 (en) 2012-09-25 2016-03-09 Cargill Inc MIXTURES OF STÉVIOSIDE
DE102012018926B4 (de) 2012-09-25 2024-08-01 Infineon Technologies Ag Blinkerschaltung zur Steuerung eines Blinkers in einem Fahrzeug
KR20150113166A (ko) 2013-01-31 2015-10-07 코덱시스, 인코포레이티드 상호작용 성분을 이용하여 생체분자를 확인하기 위한 방법, 시스템, 및 소프트웨어
WO2014122227A2 (en) 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
SG11201506127WA (en) 2013-02-11 2015-09-29 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
KR101404728B1 (ko) 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
WO2014146135A2 (en) 2013-03-15 2014-09-18 The Coca-Cola Company Steviol glycosides, their compositions and their purification
JP2016533739A (ja) 2013-07-29 2016-11-04 ダニスコ・ユーエス・インク 変異酵素
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
ES2774965T3 (es) 2013-09-27 2020-07-23 Codexis Inc Modelado predictivo a base de estructura
ES2709439T3 (es) 2013-11-01 2019-04-16 Conagen Inc Producción recombinante de glucósidos de esteviol
CN106471128A (zh) 2014-01-28 2017-03-01 百事可乐公司 一种酶法制备瑞鲍迪甙m的方法
US9803182B2 (en) * 2014-02-03 2017-10-31 The Regents Of The University Of California Ionic liquid-tolerant cellulase enzymes
WO2016028899A1 (en) 2014-08-19 2016-02-25 The Coca-Cola Company Methods for preparing rebaudioside i and uses
KR101765369B1 (ko) 2014-09-19 2017-08-08 한국과학기술원 돌외 유래의 신규한 당전이효소 및 이의 용도
CN107105734A (zh) 2014-09-19 2017-08-29 谱赛科有限责任公司 高纯度甜菊醇糖苷
US9783566B2 (en) 2014-10-03 2017-10-10 Conagen Inc. Non-caloric sweeteners and methods for synthesizing
WO2016055578A1 (en) 2014-10-08 2016-04-14 Dsm Ip Assets B.V. Steviol glycoside production
MY182396A (en) 2014-11-05 2021-01-23 Manus Biosynthesis Inc Microbial production of steviol glycosides
JP6783232B2 (ja) 2014-11-24 2020-11-11 カーギル インコーポレイテッド グリコシド組成物
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
BR112017020308A2 (pt) 2015-03-23 2018-07-10 Dsm Ip Assets Bv udp-glicosiltransferases
JP2019517801A (ja) * 2016-06-15 2019-06-27 コデクシス， インコーポレイテッド 操作されたβ−グルコシダーゼおよびグルコシル化方法

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US20190211318A1 (en) 2019-07-11
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AU2017284104A1 (en) 2018-12-20
JP2019517801A (ja) 2019-06-27
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KR20190018512A (ko) 2019-02-22
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MX2018015534A (es) 2019-03-14
US20220195411A1 (en) 2022-06-23

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