EA201600428A1 - METHOD OF CREATION, REPLACEMENT - Google Patents
METHOD OF CREATION, REPLACEMENTInfo
- Publication number
- EA201600428A1 EA201600428A1 EA201600428A EA201600428A EA201600428A1 EA 201600428 A1 EA201600428 A1 EA 201600428A1 EA 201600428 A EA201600428 A EA 201600428A EA 201600428 A EA201600428 A EA 201600428A EA 201600428 A1 EA201600428 A1 EA 201600428A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- dna construct
- growth factor
- specific
- genetic engineering
- species
- Prior art date
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Изобретение относится к медицине, а также биохимии и биотехнологии и представляет собой способ стимуляции регенерации соединительной ткани (сухожилия и связки) при травмах и заболеваниях опорно-двигательного аппарата у лошади. Способ стимуляции репаративного ангиогенеза и регенерации соединительной ткани, при её повреждении и/или заболевании, в медицине, заключающийся в том, что его выполняют введением в организм животного генно-инженерной ДНК-конструкции, в которую клонированы гены, кодирующие экспрессию видоспецифичных (для конкретного вида животного) белковых факторов, а именно сосудистого эндотелиального фактора роста изоформы 164 (VEGF164) и фактора роста фибробластов 2 (FGF2), способ по п.1 характеризуется тем, что генно-инженерная ДНК-конструкция выполнена в виде плазмидной конструкций, способ по п.1 характеризуется тем, что генно-инженерная ДНК-конструкция выполнена в виде вирусного вектора либо вирусных частиц, способ по п.1 характеризуется тем, что генно-инженерная ДНК-конструкция выполнена в любом известном виде, способ по пп.1-4, отличающийся тем, что для реализации способа используют фармацевтическую композицию, содержащую генетическую ДНК-конструкцию в эффективном количестве и фармацевтически допустимые вспомогательные вещества, а также возможно вирусы, микроорганизмы или клетки, генетическая ДНК-конструкция SEQ ID NO:1, характеризующаяся тем, что для реализации способа по п.1 используют двухкассетную экспрессионную плазмидную ДНК-конструкцию, созданную на базе вектора pBudCE4.1, в котором клонированы видоспецифично кодон-оптимизированные гены, кодирующие экспрессию видоспецифичных для лошади домашней (Equus ferus caballus) белковых факторов, а именно сосудистого эндотелиального фактора роста изоформы 164 (VEGF164) и фактора роста фибробластов 2 (FGF2).The invention relates to medicine, as well as biochemistry and biotechnology, and is a method of stimulating the regeneration of connective tissue (tendons and ligaments) in injuries and diseases of the musculoskeletal system in a horse. A method of stimulating reparative angiogenesis and regeneration of connective tissue, in case of its damage and / or disease, in medicine, which consists in introducing into the animal organism a genetically engineered DNA construct into which genes encoding species-specific expression are cloned (for a specific species animal) protein factors, namely, vascular endothelial growth factor isoform 164 (VEGF164) and fibroblast growth factor 2 (FGF2), the method according to claim 1 is characterized in that the genetic engineering DNA construct is made in de plasmid constructs, the method according to claim 1 is characterized by the fact that the genetic engineering DNA construct is made in the form of a viral vector or virus particles, the method according to claim 1 is characterized in that the genetic engineering DNA construct is made in any known form, method According to claims 1-4, characterized in that for the implementation of the method a pharmaceutical composition is used containing a genetic DNA construct in an effective amount and pharmaceutically acceptable excipients, as well as possibly viruses, microorganisms or cells, genet The DNA DNA construct SEQ ID NO: 1, characterized in that the method of claim 1 uses a two-cassette expression plasmid DNA construct based on the pBudCE4.1 vector, in which species-specific codon-optimized genes that encode expression-specific for horse domestic (Equus ferus caballus) protein factors, namely, vascular endothelial growth factor isoform 164 (VEGF164) and fibroblast growth factor 2 (FGF2).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2015154355A RU2614665C1 (en) | 2015-12-18 | 2015-12-18 | Method for reparative angiogenesis stimulation and connective tissue regeneration in case of its damage, by the genetic therapy method using species-specific vegf and fgf2 protein factors genes in veterinary medicine, and genetic structure for implementation of method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201600428A1 true EA201600428A1 (en) | 2017-06-30 |
| EA033539B1 EA033539B1 (en) | 2019-10-31 |
Family
ID=58505620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201600428A EA033539B1 (en) | 2015-12-18 | 2016-06-14 | Method of promotion of reparative angiogenesis and regeneration of damaged connective tissue by means of gene therapy using orphan genes of vegf and fgf2 protein factors in veterinary medicine, and genetic construct for realizing such method |
Country Status (2)
| Country | Link |
|---|---|
| EA (1) | EA033539B1 (en) |
| RU (1) | RU2614665C1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2719513C1 (en) * | 2019-06-07 | 2020-04-20 | федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) | Method of stimulating reparative angiogenesis and regeneration of skin of dogs with damage thereof by gene therapy using species-specific genes of protein factors vegf and fgf2 in veterinary science and genetic construct for realizing declared method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8213405B2 (en) * | 2008-02-01 | 2012-07-03 | Qualcomm Incorporated | Wireless network synchronization |
| CN101980714A (en) * | 2008-03-31 | 2011-02-23 | 奥古斯蒂努斯·巴德 | Methods and compositions for tissue regeneration by means of stem cells or bone marrow cells |
| RU2517117C2 (en) * | 2012-11-26 | 2014-05-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Казанский государственный медицинский университет" Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Казанский ГМУ Минздравсоцразвития России) | Method for stimulating neurotisation using nanostructured matrix and genetic constructs |
| RU2558294C1 (en) * | 2014-09-16 | 2015-07-27 | Общество с ограниченной ответственностью "НекстГен" | Codon-optimised recombinant plasmid, stimulation technique for peripheral nerve regeneration, treatment modality for damaged human nerve |
-
2015
- 2015-12-18 RU RU2015154355A patent/RU2614665C1/en active
-
2016
- 2016-06-14 EA EA201600428A patent/EA033539B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EA033539B1 (en) | 2019-10-31 |
| RU2614665C1 (en) | 2017-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): RU |