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EA201201132A1 - Очистка антител с помощью единого функционального блока - Google Patents

Очистка антител с помощью единого функционального блока

Info

Publication number
EA201201132A1
EA201201132A1 EA201201132A EA201201132A EA201201132A1 EA 201201132 A1 EA201201132 A1 EA 201201132A1 EA 201201132 A EA201201132 A EA 201201132A EA 201201132 A EA201201132 A EA 201201132A EA 201201132 A1 EA201201132 A1 EA 201201132A1
Authority
EA
Eurasian Patent Office
Prior art keywords
anion exchange
hydrophobic interaction
chromatography part
exchange chromatography
single functional
Prior art date
Application number
EA201201132A
Other languages
English (en)
Inventor
Дидерик Рейндер Кремер
Рандал Виллиам Марлевелд
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201201132A1 publication Critical patent/EA201201132A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретение относится к способу очистки антител от смеси белков, продуцируемой в биореакторе, по меньшей мере, включающему этапы промежуточной очистки и доочистки, где этапы промежуточной очистки и доочистки содержат последовательную обработку анионообменной хроматографией (АЕХ) и хроматографией с гидрофобным взаимодействием (HIC) в проточном режиме. Изобретение также относится к единому функциональному блоку, включающему и часть анионообменной хроматографии, и часть хроматографии с гидрофобным взаимодействием, которые соединены последовательно, причем блок включает вход на загрузочном конце части анионообменной хроматографии и выход на разгрузочном конце части хроматографии с гидрофобным взаимодействием и блок также включает входное отверстие между частью анионообменной хроматографии и частью хроматографии с гидрофобным взаимодействием.
EA201201132A 2010-02-12 2011-02-10 Очистка антител с помощью единого функционального блока EA201201132A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10153529 2010-02-12
PCT/EP2011/051975 WO2011098526A1 (en) 2010-02-12 2011-02-10 Single unit antibody purification

Publications (1)

Publication Number Publication Date
EA201201132A1 true EA201201132A1 (ru) 2013-03-29

Family

ID=42133600

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201201132A EA201201132A1 (ru) 2010-02-12 2011-02-10 Очистка антител с помощью единого функционального блока

Country Status (15)

Country Link
US (1) US20130131318A1 (ru)
EP (1) EP2534169A1 (ru)
JP (1) JP2013519652A (ru)
KR (1) KR20120118065A (ru)
CN (1) CN102762585B (ru)
AR (1) AR080163A1 (ru)
AU (1) AU2011214361C1 (ru)
BR (1) BR112012020254A2 (ru)
CA (1) CA2787897A1 (ru)
CL (1) CL2012002125A1 (ru)
EA (1) EA201201132A1 (ru)
IL (1) IL221072A0 (ru)
MX (1) MX2012009283A (ru)
TW (1) TW201144327A (ru)
WO (1) WO2011098526A1 (ru)

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Also Published As

Publication number Publication date
AR080163A1 (es) 2012-03-21
MX2012009283A (es) 2012-09-07
KR20120118065A (ko) 2012-10-25
JP2013519652A (ja) 2013-05-30
WO2011098526A1 (en) 2011-08-18
AU2011214361B2 (en) 2014-12-04
CA2787897A1 (en) 2011-08-18
TW201144327A (en) 2011-12-16
CL2012002125A1 (es) 2012-09-28
BR112012020254A2 (pt) 2016-05-03
AU2011214361A1 (en) 2012-08-23
EP2534169A1 (en) 2012-12-19
AU2011214361C1 (en) 2016-01-14
IL221072A0 (en) 2012-09-24
CN102762585A (zh) 2012-10-31
US20130131318A1 (en) 2013-05-23
CN102762585B (zh) 2016-01-20

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