EA036416B1 - Antimicrobial composition - Google Patents

Abstract

The invention relates to an antimicrobial composition comprising: a) 0.01 to 2 wt.% of thymol; b) 0.01 to 2 wt.% of terpineol; and c) 0.1 to 2 wt.% of a cationic phospholipid complex, wherein the cationic phospholipid complex comprises linoleamidopropyl PG-dimonium chloride phosphate. The invention also relates to a method of cleaning or disinfecting a surface comprising the step of applying an antimicrobial composition on to the surface. Furthermore, the invention relates to the use of a leave-on antimicrobial composition and to the use of linoleamidopropyl PG-dimonium chloride phosphate in an antimicrobial composition comprising thymol and terpineol to increase the antimicrobial activity of the composition.

Description

The technical field to which the invention relates

The present invention relates to an antimicrobial composition, and more specifically to an antimicrobial composition that does not require rinsing after use.

Prior art

People tend to take good care of the outer surface of their bodies. Specific issues related to skin care include healthy skin without infections, good skin tone, and skin hygiene. Skin hygiene is achieved mainly by protecting the skin from infections. One way to cope with an infection is by treating it with antimicrobial drugs after infection is established. Another approach is to leave a minimum amount of antimicrobial agent on the surface so that any invading microorganism can be killed or inactivated in order to minimize the spread of disease. Another approach is to improve the natural immunity of a specific surface.

The advantage of the no-rinse composition is that it remains on the skin after application. Therefore, it is possible to provide increased antibacterial activity through the use of rinse-free compositions.

Various antimicrobial compositions are known in the art.

WO 2010/046238 (Unilever) discloses an antimicrobial composition for cleansing or personal care. It is an object of the present invention to provide antimicrobial compositions having a relatively rapid antimicrobial action.

The present inventors have surprisingly found that compositions containing selected ingredients, namely thymol and terpineol, in certain proportions provide a relatively rapid antimicrobial effect.

USA 2004/0014818 (Boeck) discloses a bactericidal preparation in the form of a solution, cream or ointment, consisting of photosynthesized hydrocarbons isolated from hydrocarbons, 2-hydroxy-1-isopropyl-4-methyl benzene (thymol) and butylated hydroxytoluene, and gives examples of several compositions, each of which contains from 10 to 20 compounds with antibacterial effectiveness.

GB 508407 (Shepherd, 1938) discloses an antiseptic product and a method for its preparation, including the steps of mixing salol and thymol in a weight ratio of 1: 3, melting the mixture and cooling until crystals are formed. An example of a composition is described containing 59 parts of crystals, 41 parts of terpineol, 200 parts of red Turkish oil and 200 parts of water. The composition described in this document contains about 8 wt.% Thymol and about 8 wt.% Terpineol and is believed to be particularly useful for air disinfection.

While the prior art discloses compositions with antimicrobial activity, there is still a need to provide an anti-microbial composition that does not require rinsing, that can exhibit antimicrobial activity for a long time, and contains the minimum amount of known antimicrobial ingredients.

Therefore, an object of the present invention is to provide an antimicrobial composition.

Another object of the present invention is to provide an antimicrobial composition for rinse-free use.

Another object of the present invention is to provide an antimicrobial composition for rinse-free use having long-term antibacterial activity.

A further object of the present invention is to provide an antimicrobial composition for rinse-free applications using the minimum amount of known antimicrobial compounds.

The present inventors, working intensively in this field, unexpectedly found that a formulation with a small amount of thymol, terpineol and a cationic phospholipid complex provides an antimicrobial composition with good and long-lasting antibacterial activity, which cannot be achieved using a small amount of thymol and terpineol alone, thus satisfying one or more of the above purposes.

The essence of the invention

In a first aspect, the present invention provides an antimicrobial composition comprising:

a) 0.01 to 2 wt% thymol,

b) 0.01 to 2% by weight terpineol, and

c) 0.1 to 2% by weight of a cationic phospholipid complex.

In a second aspect, the present invention provides a method for disinfecting a surface comprising the steps of applying a composition according to the first aspect to said surface.

In a third aspect of the present invention, the antimicrobial composition comprising thymol and terpineol provides for the use of a cationic phospholipid complex to enhance antibacterial activity.

- 1 036416

Any feature of one aspect of the present invention may be used in any other aspect of the invention. The word containing includes the meaning including, but not necessarily consisting of. In other words, the steps or options listed do not need to be exhaustive. Except for working and comparative examples, or where expressly indicated otherwise, all numbers in this description indicating quantities of material or reaction conditions, physical properties of materials and / or use are to be understood in view of the changes introduced by the word about (about). The range of numeric values, expressed in the format from x to y, is understood to include x and y. Where multiple preferred ranges are described for a feature in the format x to y, it is assumed that all the limits combining the different endpoints are also considered.

Detailed description of the invention

The present invention provides an antimicrobial composition comprising:

a) 0.01 to 2% by weight of thymol;

b) 0.01 to 2% by weight terpineol, and

c) 0.1 to 2% by weight of a cationic phospholipid complex.

An antimicrobial composition, as mentioned above, preferably means any composition capable of killing or at least causing a significant reduction in the number of microbes that cause general diseases. Gram-positive organisms that cause common diseases include Staphylococcus, Streptococcus, and Enterococcus spp. Some of the gram-negative organisms that cause common diseases include Escherichia coli, Salmonella, Klebsiella, and Shigella. Escherichia coli and Salmonella can cause severe gastrointestinal illness.

The composition of the present invention is preferably in the form of a rinse-free composition. A rinse-free composition preferably means compositions that are applied to a surface (eg, skin) without the subsequent step of rinsing the surface to remove the composition after use. Compositions that remain on the surface after application are predominantly meant.

Thymol.

The antimicrobial composition of the present invention contains thymol. The amount of thymol is advantageously in the range from 0.01 to 2%, more preferably from 0.01 to 1% and even more preferably from 0.01 to 0.5%, most preferably from 0.01 to 0.1% and even more preferably 0.01 to 0.05%. The compositions of the present invention are capable of providing the desired antibacterial effect at a very low concentration of thymol. At concentrations higher than the high preferred concentrations of thymol when combined with terpineol, taking into account that the kinetic characteristics of action should not be compromised, the present inventors have found that, in contrast to therapeutic / pesticidal / herbicidal applications where sensory aspects do not play a role, critical, in the case of the present application, which is preferably a personal care application where the product is in contact with hands or other body parts, sensory aspects such as smell and sensation on the skin may be disturbed. Thymol can be added to the antimicrobial composition in purified form.

The amount of thymol used in the composition of the present invention is preferably very low. The present inventors have found that a low concentration of thymol, when combined with the other required formulation ingredients, is capable of providing a good, long-lasting and long-lasting antibacterial effect.

Alternatively, thyme oil or thyme extract containing thymol can be added to the antimicrobial composition, but care must be taken that thymol is present in the desired concentration in the composition of the present invention. Thyme oil or thyme extract is obtained from the thyme plant. The thyme plant belongs to the Thymus plant genus, which includes the following species: Thymus vulgaris, Thymus zygis, Thymus satureoides, Thymus mastichina, Thymus broussonetti, Thymus maroccanus, Thymus pallidus, Thymus algeriensis, Thymus serpyllum, and Thymus pule Thymus pule The thymol isomer (carvacrol) can also preferably be used.

- 2 036416

Terpineol.

The antimicrobial composition of the present invention also contains terpineol. The amount of terpineol is preferably in the range from 0.01 to 2%, more preferably from 0.01 to 1% and even more preferably from 0.01 to 0.5%, most preferably from 0.01 to 0.1% and further more preferably 0.01 to 0.05%. The compositions of the present invention are capable of providing the desired antibacterial effect at a very low concentration of terpineol. At concentrations higher than the high preferred concentrations of terpineol when combined with thymol, taking into account that the kinetic characteristics of exposure should not be compromised, the present inventors have found that, in contrast to therapeutic / pesticidal / herbicidal applications where sensory aspects do not play a role, critical, in the case of the present application, which is preferably a personal care application where the product is in contact with hands or other body parts, sensory aspects such as smell and sensation on the skin may be disturbed. Terpineol can be added to the antimicrobial composition in purified form.

The amount of terpineol used in the composition of the present invention is preferably very low. The present inventors have found that a low concentration of terpineol, when combined with the other required formulation ingredients, is capable of providing good, long-lasting and long-lasting antibacterial action.

The structure of terpineol is given below

Alternatively, a terpineol-containing coniferous oil can be added to the antimicrobial composition.

Cationic phospholipid complex.

The antimicrobial composition of the present invention also contains a cationic phospholipid complex, also known as a cationic phospholipid compound.

Phospholipids are a class of lipids that are the main components of all cell membranes. They have amphiphilic characteristics; therefore, they can form lipid bilayers. The structure of a phospholipid molecule typically consists of two hydrophobic fatty acid tails and a hydrophilic head.

A preferred cationic phospholipid complex of the composition of the present invention comprises phosphatide diesters.

Linoleamidopropyl PG-dimonium chloride phosphate is the most preferred cationic phospholipid complex for the composition of the present invention. It has the following structure:

ch ₃

A j ⁰ '? ^{1 =} Y ⁼ \ LLL ^L nnAL _m yL ₁ ch, ° P p

Linoleamidopropyl PG-dimonium chloride phosphate consists of several chains of predominantly diesters of phosphatides. It is isolated from safflower oil, which has a high linoleic acid content.

The amount of the phospholipid complex is in the range of 0.1 to 2%, preferably 0.1 to 1%, and most preferably 0.1 to 0.5% by weight of the composition.

The composition of the present invention also preferably contains a mixture of oils.

The term oil blend preferably means an additional oil blend, other than thymol and terpineol, which is used to provide the enhanced sensory properties of a personal care composition.

The oil will in most cases impart good sensory properties to the composition, which is a non-rinse composition. Preferably, the non-rinse composition contains oil in its composition. The composition of the present invention preferably contains from 3 to 30%,

3 036416 more preferably 5 to 30%, even more preferably 10 to 30% and most preferably 20 to 30% by weight of the oil mixture.

The oil mixture preferably contains saturated triglycerides and linear dimethicone.

Dimethicones with different viscosities can preferably be added to optimize the rheological properties of the composition.

It is preferred that the saturated triglycerides comprise a mixture of capric and caprylic triglycerides. In the most preferred situation, the ratio of capric to caprylic in the saturated triglyceride ranges from 60:40 to 80:20, and most preferably 70:30.

We have found that the addition of a small amount of the oil mixture affects the antibacterial activity of the composition of the present invention. However, with an increase in the amount of the oil mixture, the antibacterial activity is restored.

The composition of the present invention preferably contains a cosmetically acceptable base.

The cosmetically acceptable base is preferably a cream, lotion, gel or emulsion.

Personal care compositions (not requiring rinsing) can be prepared using a variety of cosmetically acceptable emulsifying or non-emulsifying systems and bases. Cream is a very suitable base. Fast absorbing creams are particularly preferred. Fast-absorbing cream bases contain 5 to 25% fatty acid and 0.1 to 10% soap. The base for fast absorbing creams gives an increased matte feeling to the skin. C12-C20 fatty acids are particularly preferred in fast absorbing cream bases, even more preferred are C14-C18 fatty acids. The most preferred fatty acid is stearic acid. The fatty acid in the composition is more preferably present in an amount ranging from 5 to 20% by weight of the composition. Soap bases for fast-absorbing creams include an alkali metal salt of fatty acids like sodium or potassium salts, most preferably potassium stearate. The soap in the fast absorbing cream base is generally present in an amount in the range of 0.1 to 10%, more preferably 0.1 to 3% by weight of the composition. Typically, a fast-absorbing cream base in personal care compositions is prepared by mixing the required amount of the total fatty substance with the required amounts of potassium hydroxide. Soap is usually formed in situ during mixing.

A particularly suitable cosmetically acceptable base is an oil-in-water emulsion containing silicone oils as a dispersion phase. The water in oil emulsions preferably contains a mixture of cross-linked silicone elastomers.

The inclusion of a mixture of silicone elastomers in a water-in-oil emulsion can be used as a cosmetically acceptable base for preparing the compositions of the present invention. When using silicone fluids, silicone crosslinked elastomers are particularly preferred. In contrast to silicone liquid polymers, the physical properties of elastomers generally depend on the number of crosslinks rather than molecular weight. The swelling properties of silicone elastomers make them ideal thickeners for oil phases. Elastomers leave a smooth and soft feeling when applied to skin or hair. They can also be used as delivery vehicles for flavors, vitamins, and other additives in cosmetic compositions.

Suitable mixtures or gels of silicone elastomers that are commercially available, suitable for inclusion in the composition of the invention, and found to provide increased stability are Dow Corning® EL-8051 IN Organic Silicone Elastomer Blend [INCI Name: Isodecyl Neopentanate (s) Dimethicone / bis-isobutyl PPG-20 crosspolymer]; EL-8050 [INCI name: isododecane (s) dimethicone / bis-isobutyl PPG 20 crosspolymer] DC 9040, DC9041, DC9045 (dimethicone crosspolymer); DC 9506, 9509 (dimethicone / vinyl dimethicone crosspolymer); Shin-Etsu KSG-15, KSG-16, KSG-17 (dimethicone / vinyl dimethicone crosspolymer). Preferably, the composition contains from 5 to 50% silicone elastomer by weight of the composition.

The composition of the invention preferably contains 0.1 to 5% by weight of nicotinamide or its derivatives. Any nicotinamide derivatives having properties similar to nicotinamide can be preferably used. In addition to nicotinamide, other well-known skin whitening agents can be used, such as aloe extract, ammonium lactate, arbutin, azelaic acid, kojic acid, butylhydroxyanisole, butylhydroxytoluene, citric acid esters, 3 diphenyl propane derivatives, 2,5 dihydroxybenzoic acid and its derivatives acid, fennel extract, glucopyranosyl-1-ascorbate, gluconic acid, glycolic acid, green tea extract, hydroquinone, hydroxyanisole and its derivatives, 4-hydroxybenzoic acid derivatives, hydroxycaprylic acid, lemon extract, linoleic acid, magnesium ascorbyl phosphate, mulberry root extract , 2,4 resorcinol derivatives, 3,5 resorcinol derivatives, salicylic acid, vitamins such as vitamin B6, vitamin B12, vitamin C, vitamin A, dicarboxylic acid, resorcinol derivatives, hydroxycarboxylic acid, lactic acid and its salts, e.g. lactate

- 4 036416 sodium and mixtures thereof.

In addition, the composition may, although not preferred, contain a sunscreen component. Any sunscreen component that is suitable for use with the base can be added. Both UVA and UVB sunscreens are preferably added.

The composition of the invention may preferably contain a UV-A sunscreen which is dibenzoylmethane or derivatives thereof. Preferred dibenzoylmethane derivatives are selected from 4-tert-butyl-4'-methoxydibenzoylmethane, 2-methyldibenzoylmethane, 4-methyl-dibenzoylmethane, 4-isopropyldibenzoylmethane, 4-tert-butyldibenzoylmethane, 2,4-dimethyldibenzoylmethane, 4-dibenzoylmethane, 4'-diisopropyldibenzoylmethane, 2-methyl-5-isopropyl-4'-methoxydibenzoylmethane, 2-methyl-5-tert-butyl-4'-methoxydibenzoylmethane, 2,4-dimethyl-4'-methoxydibenzoylmethane or 2,6-dimethyl- 4-tert-butyl-4'-methoxydibenzoylmethane. The most preferred dibenzoylmethane derivative is 4-tert-butyl-4'-methoxydibenzoylmethane. The composition of the invention preferably contains from 0.1 to 10%, more preferably from 0.2 to 5%, even more preferably from 0.4 to 3% by weight of dibenzoylmethane or a derivative thereof, based on the total weight of the composition and including all related limits.

The composition may also preferably contain a UV-B organic sunscreen selected from the class of cinnamic acid, salicylic acid, diphenylacrylic acid and their derivatives. Illustrative, non-limiting examples of UV-B sunscreens commercially available and suitable for inclusion in the composition of the invention are Octisalat ™, Homosalat ™, NeoHeliopan ™, Octocrylene ™, Oxybenzone ™, or Parsol MCX ™. The UV-B sunscreen is most preferably 2-ethyl-hexyl-4-methoxycinnamate, commercially available as Parsol MCX. The UV-B organic sunscreen is preferably included in an amount of 0.1 to 10%, more preferably 0.1 to 7% by weight of the composition. According to research, the presence of an organic UV-B sunscreen like 2-ethyl-hexyl-4-methoxycinnamate also causes a rapid degradation of the UV-A sunscreen of dibenzoylmethane in the presence of UV radiation. The presence of esters of rosmarinic acid has been found to be very effective in stabilizing the composition even when UV-B sunscreens are present.

Suitable inorganic sunscreens are also preferably used in the present invention. These include, for example, zinc oxide, iron oxide, silicon dioxide such as colloidal silicon dioxide, and titanium dioxide.

Preservatives may also be added to the compositions to protect against the growth of potentially harmful microorganisms. Suitable conventional preservatives for the compositions of this invention are parahydroxybenzoic acid alkyl esters. Other preservatives that have recently come into use include hydantoin derivatives, propionic acid salts and a number of quaternary ammonium compounds. Particularly preferred preservatives are phenoxyethanol, methylparaben, propylparaben, imidazolidinylurea, sodium dehydroacetate, benzyl alcohol, alkanediols, most preferably 1,2-octanediol and phenoxyethanol. Preservatives should be selected with consideration for the use of the composition and possible incompatibilities between preservatives and other ingredients. Preservatives are preferably used in amounts ranging from 0.01 to 2% by weight of the composition.

A variety of other optional materials can be included in the compositions. These include antimicrobial agents such as 2-hydroxy-4,2 ', 4'-trichlorodiphenylether (triclosan), 2,6-dimethyl-4-hydroxychlorobenzene, and 3,4,4'-trichlorocarbanilide; scrub and exfoliating particles such as polyethylene and silica or alumina; cooling agents such as menthol; skin soothing agents such as aloe vera and dyes.

In addition, the compositions may further comprise from 0 to 10 wt% opacifiers and pearlescent additives such as ethylene glycol distearate, titanium dioxide, or Lytron® 621 (styrene / acrylate copolymer); they are all used to improve the appearance or characteristics of a product.

Diluents other than water can include liquid or solid emollients, solvents, wetting agents, thickening agents, and powders. Examples of each of these types of carriers, which can be used alone or as mixtures of one or more carriers, are as follows.

Emollients such as stearyl alcohol, glyceryl monoricinoleate, mink fat, cetyl alcohol, isopropyl isostearate, stearic acid, isobutyl palmitate, isocetyl stearate, oleyl alcohol, isopropyl laurate, hexyl laurate, decyl oleicolate-2 alcohol, behenyl alcohol, cetyl palmitate, silicone oils such as dimethyl polysiloxane, di-p-butyl sebacate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, butyl stearate, polyethylene glycol, triethylene glycol, lanolin, cocoa butter, corn oil oil, palm kernel oil, canola oil, safflower oil, evening primrose oil,

- 5 036416 soybean oil, sunflower seed oil, avocado oil, sesame seed oil, coconut oil, peanut oil, castor oil, acetylated lanolin alcohol, liquid paraffin, mineral oil, butyl myristate, isostearic acid, palmitic acid, isopropyl linoleate , myristyl lactate, decyl oleate, myristyl myristate.

Solvents such as ethyl alcohol, isopropanol, acetone, ethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monoethyl ether.

Preferably, active agents are added to the composition in addition to the skin conditioning agents mentioned above. These active ingredients can preferably be selected from antibacterial agents, vitamins, anti-acne agents, anti-wrinkle agents, anti-atrophy agents and agents for restoring the biological functions of the skin; active substances that restore the skin barrier; cosmetic non-steroidal soothing active substances; products for artificial tanning and tanning accelerators; skin whitening products; sunscreen active ingredients; funds that stimulate the sebaceous glands; inhibitors of sebaceous gland secretion; antioxidants; protease inhibitors; skin lifting products; antipruritic ingredients; hair growth inhibitors; 5-a-reductase inhibitors; exfoliating agents; antiglycosylating agents or mixtures thereof and the like.

These active agents can be selected from water-soluble active agents, oil-soluble active agents, pharmaceutically acceptable salts, and mixtures thereof. As used herein, the term active means active ingredients for personal care that can be used for beneficial effects on the skin and / or hair and which are not generally used for conditioning the skin, such products are delivered by emollient skin cosmetics as described above. As used herein, the term “safe and effective amount” means an amount of active agent that is large enough to alter a condition requiring treatment or provide the desired skin care benefit, but small enough to avoid serious side effects. As used herein, the term benefit refers to a therapeutic, prophylactic and / or permanent benefit associated with the treatment of a particular condition with one or more of the active substances described herein. How much of the active substance (s) is safe and effective will vary depending on the specific active substance, the ability of the active substance to penetrate the skin, age, health and skin condition of the user, and other similar factors.

Powders such as chalk, talc, fuller's clay, kaolin, starch, gums, colloidal silicon dioxide, sodium polyacrylate, tetraalkyl and / or trialkylaryl ammonium smectites, chemically modified aluminum-magnesium silicate, organically modified montmorillonite clay, hydrated silicate silicate silicon, carboxyvinyl polymer, sodium carboxymethyl cellulose, ethylene glycol monostearate.

The cosmetically acceptable base usually constitutes from 10 to 99.9%, preferably from 50 to 99% by weight of the composition and, in the absence of other personal care aids, may constitute the remainder of the composition.

The composition of the invention may preferably contain a conventional deodorant agent as a cosmetically acceptable carrier. By deodorant is meant a product in the form of a stick, roll-on or propellant that is used as a personal odor control agent, for example, by application to the armpit, and which may or may not contain antiperspirant.

Deodorant compositions are generally in solid form, in the form of a soft solid, in the form of gels, creams, and liquids, and are dispensed with applicators that match the physical characteristics of the composition. Deodorant compositions that are delivered via roll-on applicators typically contain a liquid carrier. Such a liquid carrier can be hydrophobic or contain a mixture of hydrophilic and hydrophobic liquids. They can be in the form of an emulsion or microemulsion. The liquid carrier or mixture of carriers often constitutes from 30 to 95% by weight of the composition and in many cases from 40 to 80%. Hydrophobic liquid carriers generally may contain one or more substances selected within the chemical classes of siloxanes, hydrocarbons, branched aliphatic alcohols, esters and ethers, which have a melting point of not more than 25 ° C and a boiling point of at least 100 ° C. ... Hydrophilic liquid carriers that can be used in the compositions typically contain water and / or one or a polyhydric alcohol or water-soluble homologue. Monohydric alcohols are often short chain alcohols, meaning that they contain up to 6 carbon atoms, and in practice it is most often ethanol or sometimes isopropanol. Polyhydric alcohols usually include ethylene or propylene glycol, or a homologue such as diethylene glycol can be used. In addition, other suitable carrier or component used for the deodorant composition can be added.

When the composition is part of a hand sanitizer composition, a cosmetic 6 036416 base may be composed of alcohol and water. The most preferred alcohols are ethyl alcohol and isopropyl alcohol. Even more preferably, a mixture of two or more alcohols is used in the hand sanitizer composition. The amount of alcohol is preferably in the range of 50 to 95%, more preferably 60 to 80%, and most preferably 65 to 80% by weight of the hand sanitizer.

The compositions of the present invention may contain a wide variety of other optional components. The CTFA Personal care Ingredient Handbook, Second Edition, 1992, which is incorporated by reference in its entirety, describes a wide range of non-limiting personal care ingredients and pharmaceutical ingredients commonly used in the manufacture of products. skin care products that are suitable for use in the compositions of the present invention. Examples include antioxidants, binders, dietary supplements, buffering agents, colorants, thickeners, polymers, pore-tightening agents, fragrances, moisturizers, opacifiers, conditioning agents, pH adjusters, natural extracts, essential oils, skin feel enhancers , skin soothing and skin healing products.

The composition of the present invention may also contain one or more of the following ingredients, for example, benzethonium chloride (BEC), benzalkonium chloride (BKC), chloroxylenol, zinc pyrithione (ZPT), creatine and creatinine.

The present invention also discloses a method for disinfecting a surface comprising the steps of applying a composition according to the invention to said surface.

The present invention also discloses the use of the composition of the present invention as described above for the purpose of improving antibacterial action. The improved antibacterial effect preferably means that after the composition of the present invention is applied, the amount of microbes remaining on the surface is significantly reduced. Therefore, the composition of the present invention is capable of providing a prolonged / long-term antibacterial effect. A preferred intended use of the composition of the present invention is non-therapeutic and / or cosmetic use.

The present invention also relates to the use of a cationic phospholipid complex in an antimicrobial composition containing thymol and terpineol to increase antibacterial activity. Preferably, the cationic phospholipid complex, thymol and terpineol are present in amounts capable of synergistically providing antibacterial activity. Preferably, the use relates to the use in a composition containing 0.01 to 2% by weight thymol and 0.01 to 2% by weight terpineol. For use according to the present invention, the cationic phospholipid complex is preferably present in an amount of 0.1 to 2 wt%. In the description, all percentages are percentages by weight of the entire composition. The cationic phospholipid complex preferably contains diesters of phosphatides. More preferably, the cationic phospholipid complex is linoleamidopropyl PG-dimonium chloride phosphate. The use of the antimicrobial composition according to the first aspect and the cationic phospholipid complex included therein as described hereinabove has the same advantages as the use of the phospholipid complex in the antimicrobial composition according to the third aspect of the invention.

The invention will be further demonstrated by the following non-limiting examples.

Examples of

To test efficacy, various non-rinse formulations were prepared as follows.

Example A.

For this example, the base composition was prepared according to the following table. one.

Table 1 Ingredient (wt%)

Glycerin 3

1,3-butylene glycol 2

Stearic acid 0.1

Disodium EDTA 0.1

Methacrylate copolymer 0.25

Ethanol 3

Ammonium acryloyldimethyl taurate 1,2

Water up to 100

- 7 036416

Used 1,3-butylene glycol from Calanese®, methacrylate copolymer and ammonium acryloyldimethyl taurate from Clariant®.

Example B.

To the base composition of Example 1 was added a 0.1% mixture of thymol and terpineol (0.05% thymol and

0.05% terpineol). The additional amount was adjusted with water.

Example 1.

In this example, an additional 0.5% linoleamidopropyl PG-dimonium phosphate chloride (available from Croda under the trade name Arlasilk PFA) was added to the composition of Example B. The additional amount was adjusted with water.

Example 2.

In this example, an additional 6.75% oil blend was added to the composition of Example 1. For this purpose, an oil blend was prepared by mixing 6.25% linear dimethicone of various viscosities (6% DC200-10cst from Dow Corning and 0.25% DC200-50cst from Dow Corning) and 0.5% capric / caprylic triglycerides (from Croda under the GTCC brand name). The additional amount was adjusted with water.

Example 3.

This example is the same as example 2, except that a 13.5% oil mixture was used instead of 6.75. For this purpose, an oil blend was prepared by mixing 12.5% linear dimethicone of varying viscosity (12% DC200-10cst and 0.5% DC200-50cst) and 1% capric / caprylic triglycerides (GTCC). The additional amount was adjusted with water.

Example 4.

This example is the same as example 2, except that a 27% oil mixture was used instead of 6.75. For this purpose, an oil blend was prepared by mixing 25% linear dimethicone of varying viscosity (24% DC200-10cst and 1% DC200-50cst) and 2% capric / caprylic triglycerides (GTCC). The additional amount was adjusted with water.

In vitro killing time test for bacteria using non-rinse compositions.

Bacteria tested: S. epidermidis ATCC 12228.

Testing conditions.

The compositions prepared as above were added as they are, without dilution.

The experiment temperature was 35 ± 2 ° C.

The verified contact times were 1 h and 2 h.

Preparation of bacteria culture.

Starting from the original source, no more than five passages of the tested organisms were carried out. The original culture was transferred to a growth medium and left for 24 h at 35 ± 2 ° C in an incubator shaker. The growth medium used was Tryptic Soy Broth from Difco®. A glycerol stock solution of this culture was prepared by adding the culture to 50% glycerin in a 1: 1 volume ratio. From glycerol stock solutions, the test organism was streaked onto a TSA slant (Tryptic Soy Agar, Difco®). The slant was then incubated at 35 ± 2 ° C for about 24 hours. The culture was streaked onto a TSA dish and re-incubated at 35 ± 2 ° C for about 18 hours. The culture was then carefully scraped off the dish and resuspended in sterile saline. in sufficient quantity to obtain a final suspension of at least 1x108 CFU / ml (OD at 620 nm).

Verification procedure.

For each composition, a 10 g sample was taken, about 2.5x107 test bacteria were added (as prepared above) and vortexed thoroughly for 30 seconds. Thereafter, these samples were incubated at about 37 ° C in an incubator (Bacteriological Incubator, Labtop®) and after 1 hour and 2 hours samples were taken for further testing.

After incubation (i.e. after 1 h or 2 h, respectively), 1 g of the test sample was weighed and added to 9 ml of D / E diluent (D / E neutralizer, Difco®), vortexed for about 30 s and left for about 5 min. Serial tenfold dilutions of neutralized samples were prepared by taking 1 ml of neutralized samples and using 9.0 ml of dilution medium (D / E neutralizer, Difco®). 1 ml of each diluted sample was placed in duplicate on a TSA plate and incubated at 35 ± 2 ° C for 48 h.

The control sample for this test was a sample containing only bacteria (no compositions).

After 48 hours, the bacterial colonies were counted and multiplied by the dilution factor to calculate CFU / ml. The score was converted to log10 values and, accordingly, the Log reduction was calculated by subtracting the original value from the test value. To test efficacy, various non-rinse compositions were prepared as indicated below.

The results of these experiments are summarized below in table. 2.

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Example No. Log reduction 1 hour 2 hours A 0.1 ± 0.15 0.09 ± 0.12 IN 0.03 ± 0.17 0.02 ± 0.13 one 1.0 ± 0.29 3.78 ± 0.83 2 0.45 ± 0.1 2.57 ± 0.46 3 0.63 ± 0.14 2.91 ± 0.74 4 0.8 ± 0.12 3.2 ± 0.44

table 2

From the table above, it can be seen that compositions within the scope of the present invention (examples 1-4) provide significantly improved protection against microbes compared to compositions outside the scope of the present invention (examples A and B). It is also obvious that by adding a small amount of oil mixture (example 2), the antibacterial effect is slightly reduced (compared to example 1). However, with an increase in the amount of the oil mixture (example 4), the efficiency is restored. However, although the performance of Example 2 is less than that of Example 1, it is still much better than the performance of the test cases (Examples A and B).

Claims (12)

CLAIM 1. Antimicrobial composition containing: a) 0.01 to 2% by weight thymol; b) 0.01 to 2% by weight terpineol, and c) 0.1 to 2 wt% of a cationic phospholipid complex, the cationic phospholipid complex being linoleamidopropyl PG dimonium chloride phosphate. 2. A composition according to claim 1, wherein the amount of thymol is in the range from 0.01 to 0.5% by weight. 3. A composition according to any of the preceding claims, wherein the amount of terpineol is in the range of 0.01 to 0.5% by weight. 4. A composition according to any of the preceding claims comprising 3 to 30% by weight of an oil mixture. 5. The composition of claim 4, wherein the oil mixture contains saturated triglycerides and linear dimethicone. 6. The composition of claim 5, wherein the saturated triglycerides comprise a mixture of capric and caprylic triglycerides. 7. The composition of claim 6, wherein the ratio of capric to caprylic triglyceride ranges from 60:40 to 80:20. 8. A composition according to any of the preceding claims further comprising a cosmetically acceptable base. 9. A composition according to any one of the preceding claims, further comprising 0.1 to 5% by weight of nicotinamide. 10. A composition according to any one of the preceding claims, in the form of a rinse-free composition. 11. A method for cleaning or disinfecting a surface, comprising the step of applying a composition according to any one of the preceding claims to said surface. 12. The use of linoleamidopropyl PG-dimonium chloride phosphate in an antimicrobial composition containing thymol and terpineol to increase the antimicrobial activity of the composition.