DK3382019T3 - Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri - Google Patents
Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri Download PDFInfo
- Publication number
- DK3382019T3 DK3382019T3 DK16868704.4T DK16868704T DK3382019T3 DK 3382019 T3 DK3382019 T3 DK 3382019T3 DK 16868704 T DK16868704 T DK 16868704T DK 3382019 T3 DK3382019 T3 DK 3382019T3
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- Denmark
- Prior art keywords
- converting
- nucleic acid
- specific dna
- acid base
- molecular complex
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- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015232379 | 2015-11-27 | ||
| JP2016134613 | 2016-07-06 | ||
| PCT/JP2016/085075 WO2017090761A1 (ja) | 2015-11-27 | 2016-11-25 | 標的化したdna配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3382019T3 true DK3382019T3 (da) | 2022-05-30 |
Family
ID=58764366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16868704.4T DK3382019T3 (da) | 2015-11-27 | 2016-11-25 | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US11220693B2 (da) |
| EP (1) | EP3382019B1 (da) |
| JP (1) | JP6923205B2 (da) |
| KR (1) | KR102061438B1 (da) |
| CN (1) | CN108495932B (da) |
| DK (1) | DK3382019T3 (da) |
| ES (1) | ES2914623T3 (da) |
| WO (1) | WO2017090761A1 (da) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL3115457T3 (pl) | 2014-03-05 | 2020-01-31 | National University Corporation Kobe University | Sposób modyfikacji sekwencji genomowej do specyficznego przekształcania zasad kwasu nukleinowego docelowej sekwencji dna oraz kompleks molekularny do zastosowania w nim |
| US10767173B2 (en) * | 2015-09-09 | 2020-09-08 | National University Corporation Kobe University | Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same |
| WO2017090761A1 (ja) | 2015-11-27 | 2017-06-01 | 国立大学法人神戸大学 | 標的化したdna配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体 |
| CA3021281C (en) * | 2016-04-21 | 2021-07-27 | National University Corporation Kobe University | Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor |
| CN109152343A (zh) | 2016-05-13 | 2019-01-04 | 株式会社钟化 | 转化植物的制备方法 |
| WO2017195906A1 (ja) * | 2016-05-13 | 2017-11-16 | 株式会社カネカ | 植物のゲノム編集方法 |
| US11499158B2 (en) | 2016-05-13 | 2022-11-15 | Kaneka Corporation | Method for modifying plant |
| CN107043779B (zh) * | 2016-12-01 | 2020-05-12 | 中国农业科学院作物科学研究所 | 一种CRISPR/nCas9介导的定点碱基替换在植物中的应用 |
| JPWO2018143477A1 (ja) * | 2017-02-06 | 2019-04-25 | 国立大学法人 筑波大学 | 双子葉植物のゲノムの改変方法 |
| CN110157727A (zh) * | 2017-12-21 | 2019-08-23 | 中国科学院遗传与发育生物学研究所 | 植物碱基编辑方法 |
| BR112020019301A2 (pt) * | 2018-03-26 | 2021-01-05 | National University Corporation Kobe University | Método para modificar um sítio alvo de um dna de fita dupla de uma célula. |
| CN112105732A (zh) * | 2018-05-10 | 2020-12-18 | 先正达参股股份有限公司 | 用于多核苷酸的靶向编辑的方法和组合物 |
| IL280951B2 (en) | 2018-08-23 | 2024-08-01 | Sangamo Therapeutics Inc | Engineered target-specific base editors |
| CN110607320B (zh) * | 2018-11-23 | 2023-05-12 | 电子科技大学 | 一种植物基因组定向碱基编辑骨架载体及其应用 |
| CN110257406B (zh) * | 2019-06-21 | 2022-07-22 | 安徽省农业科学院水稻研究所 | 密码子植物化改造的Plant Nme2Cas9基因及其应用 |
| WO2021046155A1 (en) | 2019-09-03 | 2021-03-11 | Voyager Therapeutics, Inc. | Vectorized editing of nucleic acids to correct overt mutations |
| US20220298518A1 (en) * | 2019-09-10 | 2022-09-22 | Kaneka Corporation | Plant modification method using axillary bud meristem |
| CN115087739A (zh) * | 2020-01-03 | 2022-09-20 | 株式会社钟化 | 植物的修饰方法 |
| JPWO2022158561A1 (da) * | 2021-01-22 | 2022-07-28 | ||
| JPWO2022181796A1 (da) * | 2021-02-26 | 2022-09-01 | ||
| EP4347845A2 (en) * | 2021-05-26 | 2024-04-10 | The Board of Trustees of the University of Illinois | C4 plants with increased photosynthetic efficiency |
| EP4355902A4 (en) * | 2021-06-17 | 2025-04-30 | The Board Of Trustees Of The University Of Illinois | Crispr cascade |
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| JP2003524368A (ja) | 1997-08-26 | 2003-08-19 | アリアド ジーン セラピューティクス インコーポレイテッド | ニ量化ドメイン、三量化ドメインまたは四量化ドメインおよび補足的非相同転写活性化ドメイン、転写抑制ドメイン、dna結合ドメインまたはリガンド結合ドメインを含む融合蛋白 |
| BR0307383A (pt) | 2002-01-23 | 2005-04-26 | Univ Utah Res Found | Mutagênese cromossomica alvo utilizando nucleases de ramificações de zinco |
| DK2860267T3 (da) | 2007-03-02 | 2019-04-23 | Dupont Nutrition Biosci Aps | Kulturer med forbedret fagresistens |
| WO2010132092A2 (en) | 2009-05-12 | 2010-11-18 | The Scripps Research Institute | Cytidine deaminase fusions and related methods |
| US8772008B2 (en) | 2009-05-18 | 2014-07-08 | Sangamo Biosciences, Inc. | Methods and compositions for increasing nuclease activity |
| US20110104787A1 (en) | 2009-11-05 | 2011-05-05 | President And Fellows Of Harvard College | Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences |
| JP2013513389A (ja) | 2009-12-10 | 2013-04-22 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | Talエフェクターに媒介されるdna修飾 |
| JP2013128413A (ja) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Pprモチーフを利用したrna結合性蛋白質の改変方法 |
| EP2784157B1 (en) | 2011-10-21 | 2019-09-18 | Kyushu University, National University Corporation | Design method for rna-binding protein using ppr motif, and use thereof |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| SG11201405956SA (en) | 2012-03-23 | 2014-11-27 | Cellectis | Method to overcome dna chemical modifications sensitivity of engineered tale dna binding domains |
| PE20190842A1 (es) | 2012-05-25 | 2019-06-17 | Emmanuelle Charpentier | Arn de direccion a adn de dos moleculas |
| US20150315576A1 (en) | 2012-11-01 | 2015-11-05 | Massachusetts Institute Of Technology | Genetic device for the controlled destruction of dna |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| ES2536353T3 (es) * | 2012-12-12 | 2015-05-22 | The Broad Institute, Inc. | Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias |
| CN114634950A (zh) | 2012-12-12 | 2022-06-17 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
| AU2014248208C1 (en) | 2013-04-05 | 2023-04-27 | Corteva Agriscience Llc | Methods and compositions for integration of an exogenous sequence within the genome of plants |
| US9873907B2 (en) * | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| PL3115457T3 (pl) | 2014-03-05 | 2020-01-31 | National University Corporation Kobe University | Sposób modyfikacji sekwencji genomowej do specyficznego przekształcania zasad kwasu nukleinowego docelowej sekwencji dna oraz kompleks molekularny do zastosowania w nim |
| KR102781429B1 (ko) | 2014-10-31 | 2025-03-18 | 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 | 변형된 t 세포에서 유전자 발현의 변경 및 그의 용도 |
| ES2800168T3 (es) | 2014-11-04 | 2020-12-28 | Univ Kobe Nat Univ Corp | Procedimiento de modificación de una secuencia genómica para introducir una mutación específica en una secuencia de ADN diana por reacción de eliminación de base, y complejo molecular usado en el mismo |
| EP3348636B1 (en) | 2015-09-09 | 2021-12-01 | National University Corporation Kobe University | Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method |
| US10767173B2 (en) | 2015-09-09 | 2020-09-08 | National University Corporation Kobe University | Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same |
| AU2016342380B2 (en) * | 2015-10-23 | 2022-04-07 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| WO2017090761A1 (ja) | 2015-11-27 | 2017-06-01 | 国立大学法人神戸大学 | 標的化したdna配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体 |
| CA3021281C (en) | 2016-04-21 | 2021-07-27 | National University Corporation Kobe University | Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor |
-
2016
- 2016-11-25 WO PCT/JP2016/085075 patent/WO2017090761A1/ja not_active Ceased
- 2016-11-25 CN CN201680079887.5A patent/CN108495932B/zh active Active
- 2016-11-25 JP JP2017552750A patent/JP6923205B2/ja active Active
- 2016-11-25 KR KR1020187017539A patent/KR102061438B1/ko active Active
- 2016-11-25 US US15/779,120 patent/US11220693B2/en active Active
- 2016-11-25 EP EP16868704.4A patent/EP3382019B1/en active Active
- 2016-11-25 DK DK16868704.4T patent/DK3382019T3/da active
- 2016-11-25 ES ES16868704T patent/ES2914623T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| HK1256888A1 (en) | 2019-10-04 |
| EP3382019A1 (en) | 2018-10-03 |
| JPWO2017090761A1 (ja) | 2018-10-25 |
| US20190085342A1 (en) | 2019-03-21 |
| EP3382019A4 (en) | 2019-04-24 |
| BR112018010681A2 (pt) | 2018-11-13 |
| JP6923205B2 (ja) | 2021-08-18 |
| WO2017090761A1 (ja) | 2017-06-01 |
| US11220693B2 (en) | 2022-01-11 |
| ES2914623T3 (es) | 2022-06-14 |
| KR102061438B1 (ko) | 2019-12-31 |
| EP3382019B1 (en) | 2022-05-04 |
| CN108495932A (zh) | 2018-09-04 |
| KR20180081811A (ko) | 2018-07-17 |
| BR112018010681A8 (pt) | 2019-02-26 |
| CN108495932B (zh) | 2022-08-09 |
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