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DK3299459T3 - Staphylococcus-lyase og anvendelse deraf - Google Patents

Staphylococcus-lyase og anvendelse deraf Download PDF

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Publication number
DK3299459T3
DK3299459T3 DK16795761.2T DK16795761T DK3299459T3 DK 3299459 T3 DK3299459 T3 DK 3299459T3 DK 16795761 T DK16795761 T DK 16795761T DK 3299459 T3 DK3299459 T3 DK 3299459T3
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Denmark
Prior art keywords
lyase
staphylococcus
staphylococcus lyase
Prior art date
Application number
DK16795761.2T
Other languages
English (en)
Inventor
Hongping Wei
Hang Yang
Junping Yu
Original Assignee
Phagelux Inc
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Publication of DK3299459T3 publication Critical patent/DK3299459T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462Lysozyme (3.2.1.17)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43Enzymes; Proenzymes; Derivatives thereof
    • A61K38/51Lyases (4)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485Exopeptidases (3.4.11-3.4.19)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
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    • C12N9/88Lyases (4.)
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14Streptococcus; Staphylococcus
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
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  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK16795761.2T 2015-05-18 2016-04-12 Staphylococcus-lyase og anvendelse deraf DK3299459T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510252947.6A CN104805066B (zh) 2015-05-18 2015-05-18 一种葡萄球菌裂解酶及其应用
PCT/CN2016/079044 WO2016184274A1 (zh) 2015-05-18 2016-04-12 一种葡萄球菌裂解酶及其应用

Publications (1)

Publication Number Publication Date
DK3299459T3 true DK3299459T3 (da) 2021-01-11

Family

ID=53690268

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16795761.2T DK3299459T3 (da) 2015-05-18 2016-04-12 Staphylococcus-lyase og anvendelse deraf

Country Status (7)

Country Link
US (1) US10626387B2 (da)
EP (1) EP3299459B1 (da)
CN (1) CN104805066B (da)
AU (1) AU2016263700B2 (da)
CA (1) CA2984582C (da)
DK (1) DK3299459T3 (da)
WO (1) WO2016184274A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1212208A1 (en) 2012-05-09 2016-06-10 Contrafect Corporation Biofilm prevention, disruption and treatment with bacteriophage lysin
CN104805066B (zh) * 2015-05-18 2018-04-24 武汉菲吉乐科生物科技有限公司 一种葡萄球菌裂解酶及其应用
JP7085482B2 (ja) * 2016-01-12 2022-06-16 イントロン バイオテクノロジー,インコーポレイテッド スタフィロコッカス感染症のための凍結乾燥製剤を製造する方法
CN110684760A (zh) * 2019-09-27 2020-01-14 吉林大学 一种杀灭葡萄球菌的基因工程裂解酶及制备方法和应用
CN110592057B (zh) * 2019-09-27 2022-01-28 昆明理工大学 嵌合裂解酶ILTphg和编码此酶的多核苷酸
CN118109445A (zh) * 2024-03-22 2024-05-31 广西中医药大学 一种耐甲氧西林金黄色葡萄球菌噬菌体裂解酶LysLP-122及其制备方法和应用

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JPWO2004020635A1 (ja) * 2002-08-28 2005-12-15 株式会社テクノネットワーク四国 バクテリオファージおよび細菌感染症治療剤
DE102006061002A1 (de) * 2006-12-22 2008-06-26 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von gram-positiven Bakterien
US8883772B2 (en) * 2007-10-09 2014-11-11 Sopharmia, Inc. Broad spectrum beta-lactamase inhibitors
WO2010002959A2 (en) * 2008-07-03 2010-01-07 The Rockefeller University A chimeric bacteriophage lysin with activity against staphylococci bacteria
US8102078B2 (en) * 2008-07-14 2012-01-24 Sefe, Inc. Dynamic electrical converter system
US8481289B2 (en) * 2008-07-24 2013-07-09 The United States Of America, As Represented By The Secretary Of Agriculture Triple acting antimicrobials that are refractory to resistance development
EP2157100A1 (en) * 2008-08-19 2010-02-24 Profos AG Artificial peptidoglycan lysing enzymes and peptidoglycan binding proteins
JP5732397B2 (ja) * 2008-10-10 2015-06-10 ルソメディカメンタ, エス.エー.Lusomedicamenta, S.A. 抗菌性ファージペプチド及びその使用法
KR101016918B1 (ko) * 2009-01-08 2011-02-25 주식회사 인트론바이오테크놀로지 박테리아 특이적 넓은 항균 활성을 갖는 신규한 리신 단백질
EP2338916A1 (en) * 2009-12-23 2011-06-29 Hyglos Invest GmbH Chimeric polypeptides and their use in bacterial decoloniation
EP2397548A1 (en) * 2010-06-18 2011-12-21 Hyglos Invest GmbH Methods of generating and screening for lytic chimeric polypeptides
MX336427B (es) * 2011-04-21 2016-01-18 Univ Rockefeller Lisinas de bacteriofago de estreptococcus para la deteccion y tratamiento de bacterias gram positivas.
US8790639B2 (en) * 2012-03-28 2014-07-29 The United States Of America, As Represented By The Secretary Of Agriculture Enhanced antimicrobial lytic activity of a chimeric Ply187 endolysin
HK1212208A1 (en) * 2012-05-09 2016-06-10 Contrafect Corporation Biofilm prevention, disruption and treatment with bacteriophage lysin
CN102676490B (zh) 2012-05-21 2013-07-31 江苏省农业科学院 一种金黄色葡萄球菌噬菌体溶壁酶及其制备方法和应用
US8980614B2 (en) * 2012-09-06 2015-03-17 The United States Of America, As Represented By The Secretary Of Agriculture Staphylococcus haemolyticus prophage φSH2 endolysin is lytic for Staphylococcus aureus
CN103122347B (zh) * 2013-02-01 2015-04-22 中国科学院武汉病毒研究所 一种杀灭葡萄球菌的裂解酶及其应用
US9872893B2 (en) * 2014-07-10 2018-01-23 University Of Maryland College Park Endolysins active against Staphylococcus bacteria, pharmaceutical compositions, and methods relating thereto
CN104726439B (zh) * 2015-04-13 2017-11-14 武汉菲吉乐科生物科技有限公司 一种广谱的链球菌裂解酶及其应用
CN104805066B (zh) * 2015-05-18 2018-04-24 武汉菲吉乐科生物科技有限公司 一种葡萄球菌裂解酶及其应用
RU2724545C2 (ru) * 2015-09-17 2020-06-23 Контрафект Корпорейшн Полипептиды лизина, активные против грамотрицательных бактерий
CN111918960B (zh) * 2017-12-05 2025-01-28 比奥普来克斯有限公司 预防微生物感染的方法和组合物

Also Published As

Publication number Publication date
WO2016184274A1 (zh) 2016-11-24
CN104805066B (zh) 2018-04-24
CN104805066A (zh) 2015-07-29
EP3299459B1 (en) 2020-10-07
AU2016263700A1 (en) 2017-11-30
CA2984582A1 (en) 2016-11-24
AU2016263700B2 (en) 2021-12-02
CA2984582C (en) 2022-07-12
US20180291357A1 (en) 2018-10-11
US10626387B2 (en) 2020-04-21
EP3299459A1 (en) 2018-03-28
EP3299459A4 (en) 2018-12-19

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