DK173445B1 - Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba - Google Patents

Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba Download PDF

Info

Publication number: DK173445B1
Authority: DK; Denmark
Prior art keywords: nuclease; serratia spp; enzyme; serratia; dna
Prior art date: 1985-05-10

Application number

DK198700097A

Other languages

Danish (da)

English (en)

Other versions

DK9787D0 (da

DK9787A (da

Inventor

Soeren Molin

Michael Givskov

Erik Riise

Original Assignee

Nycomed Danmark As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-05-10

Filing date

1987-01-09

Publication date

2000-11-06

1985-05-10 Priority claimed from DK210085A external-priority patent/DK210085D0/da

1985-12-23 Priority claimed from DK606085A external-priority patent/DK606085D0/da

1987-01-09 Application filed by Nycomed Danmark As filed Critical Nycomed Danmark As

1987-01-09 Priority to DK198700097A priority Critical patent/DK173445B1/da

1987-01-09 Publication of DK9787D0 publication Critical patent/DK9787D0/da

1987-01-09 Publication of DK9787A publication Critical patent/DK9787A/da

2000-11-06 Application granted granted Critical

2000-11-06 Publication of DK173445B1 publication Critical patent/DK173445B1/da

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Tropical Medicine & Parasitology (AREA)
Virology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Jellies, Jams, And Syrups (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Compounds Of Unknown Constitution (AREA)
Bakery Products And Manufacturing Methods Therefor (AREA)

DK198700097A 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba DK173445B1 (da)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
DK198700097A DK173445B1 (da)	1985-05-10	1987-01-09	Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Applications Claiming Priority (8)

Application Number	Priority Date	Filing Date	Title
DK210085		1985-05-10
DK210085A DK210085D0 (da)	1985-05-10	1985-05-10	Fremgangsmaade til fremstilling af bakterielle enzymer
DK606085A DK606085D0 (da)	1985-12-23	1985-12-23	Fremgangsmaade til fremstilling af et bakterielt enzym
DK606085		1985-12-23
DK8600051		1986-05-09
PCT/DK1986/000051 WO1986006743A1 (en)	1985-05-10	1986-05-09	Bacterial enzymes
DK198700097A DK173445B1 (da)	1985-05-10	1987-01-09	Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba
DK9787		1987-01-09

Publications (3)

Publication Number	Publication Date
DK9787D0 DK9787D0 (da)	1987-01-09
DK9787A DK9787A (da)	1987-01-09
DK173445B1 true DK173445B1 (da)	2000-11-06

Family

ID=26066359

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198700097A DK173445B1 (da)	1985-05-10	1987-01-09	Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Country Status (13)

Country	Link
EP (1)	EP0229866B1 (es)
KR (1)	KR930002887B1 (es)
CN (1)	CN1008377B (es)
AT (1)	ATE83264T1 (es)
AU (2)	AU594062B2 (es)
CA (1)	CA1327328C (es)
DE (2)	DE3687266T2 (es)
DK (1)	DK173445B1 (es)
ES (2)	ES8800346A1 (es)
FI (1)	FI870052A0 (es)
IE (1)	IE59296B1 (es)
IL (1)	IL78703A (es)
WO (1)	WO1986006743A1 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3939771A1 (de) *	1989-12-01	1991-06-06	Behringwerke Ag	Verfahren zur biologischen inaktivierung von nukleinsaeuren
IL103846A0 (en) *	1991-11-25	1993-04-04	Tanabe Seiyaku Co	Gene coding for esterase and novel microorganism containing said gene
US5834233A (en) *	1992-04-06	1998-11-10	Gx Biosystems A/S	Method of limiting the survival of genetically engineered microorganisms in their enivronment
CH682541A5 (de) *	1992-06-23	1993-10-15	Urs Viktor Dr Wirth	Biochemische Inaktivierung von RNA-Viren.
CA2137728C (en) *	1993-12-14	1998-07-14	William E. Keating	Enzymatic removal of non-target nucleic acid from biological samples
US5968777A (en) *	1996-04-18	1999-10-19	Smithkline Beecham Corporation	DNA encoding seryl t-RNA synthetase
PT1539798E (pt)	2002-09-06	2011-02-23	Genentech Inc	Processo para extracção de proteínas
US20100092969A1 (en)	2007-03-07	2010-04-15	On-Chip Cellomics Consortium	Cell isolation method, cell testing method and reagent kit therefor
EP2108956A1 (en)	2008-04-07	2009-10-14	Erasmus University Medical Center Rotterdam	Improved methods and kits for detecting tumor-specific fusion proteins
JP5615271B2 (ja)	2008-06-18	2014-10-29	オックスフォードバイオメディカ（ユーケー）リミテッド	ウイルス精製法
DK2462224T3 (en)	2009-08-03	2017-09-04	C-Lecta Gmbh	PROCEDURE FOR MANUFACTURING NUCLEASES OF A GRAM NEGATIVE BACTERY WHEN USING A GRAM POSITIVE EXPRESSION HOST
US9599590B2 (en)	2012-10-12	2017-03-21	Sage Science, Inc.	Side-eluting molecular fractionator
CN103205448B (zh) *	2013-02-18	2016-03-23	徐东	一种裂解时自动降解核酸的大肠杆菌制备的方法
CN104099310B (zh) *	2013-04-12	2021-06-18	杭州俊丰生物工程有限公司	一种重组核酸酶及其制备方法
EP3207163B1 (en)	2014-10-15	2020-05-27	Sage Science, Inc.	Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US10655122B2 (en)	2015-04-20	2020-05-19	Qiagen Gmbh	Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample
CN108699101B (zh)	2015-11-20	2022-04-19	塞奇科学股份有限公司	用于基因组dna片段的靶向纯化的制备型电泳方法
WO2017139669A1 (en) *	2016-02-11	2017-08-17	Sage Science, Inc.	Workflows for enzymatic nucleic acid modification
JP2020515250A (ja)	2017-04-07	2020-05-28	セージサイエンス，インコーポレイテッド	統合型電気泳動ｄｎａ精製を使用する遺伝的構造変動を検出するためのシステムおよび方法
CA3099497A1 (en)	2018-05-15	2019-11-21	Flagship Pioneering Innovations V, Inc.	Fusosome compositions and uses thereof
MX2021000308A (es)	2018-07-09	2021-06-08	Flagship Pioneering Innovations V Inc	Composiciones de fusosoma y usos de estas.
JP7676305B2 (ja)	2018-11-14	2025-05-14	フラッグシップパイオニアリングイノベーションズブイ，インコーポレイテッド	Ｃｎｓ送達のためのフソソーム組成物
WO2020102485A1 (en)	2018-11-14	2020-05-22	Flagship Pioneering Innovations V, Inc.	Fusosome compositions for hematopoietic stem cell delivery
WO2020102503A2 (en)	2018-11-14	2020-05-22	Flagship Pioneering Innovations V, Inc.	Fusosome compositions for t cell delivery
CA3124535A1 (en)	2018-12-21	2020-06-25	The Research Foundation For Microbial Diseases Of Osaka University	Botulinum toxin producing method
EP3926039A1 (en)	2020-06-17	2021-12-22	AB Enzymes GmbH	Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5315491A (en) *	1976-07-23	1978-02-13	Rikagaku Kenkyusho	Preparation of novel nucleicacidase
US4174400A (en) *	1978-09-13	1979-11-13	Merck & Co., Inc.	Anthelmintic benzimidazoles
GB2121054B (en) *	1982-05-25	1986-02-26	Lilly Co Eli	Cloning vectors for expression of exogenous protein
JPS59183688A (ja) *	1983-03-31	1984-10-18	Tanabe Seiyaku Co Ltd	Ｌ−アスパラギン酸の製法
EP0131418A1 (en) *	1983-07-01	1985-01-16	Celltech Limited	Proteins, pharmaceutical compositons, genes, vectors, host organisms and processes for their production
DE3334847A1 (de) *	1983-09-27	1985-04-04	Hoechst Ag, 6230 Frankfurt	Immobilisierte 5'-phosphodiesterase, verfahren zu ihrer herstellung und ihre verwendung

1986
- 1986-05-06 IL IL78703A patent/IL78703A/xx not_active IP Right Cessation
- 1986-05-08 CA CA000508730A patent/CA1327328C/en not_active Expired - Lifetime
- 1986-05-09 DE DE8686106348T patent/DE3687266T2/de not_active Expired - Lifetime
- 1986-05-09 FI FI870052A patent/FI870052A0/fi not_active Application Discontinuation
- 1986-05-09 AU AU59100/86A patent/AU594062B2/en not_active Expired
- 1986-05-09 EP EP86106348A patent/EP0229866B1/en not_active Expired - Lifetime
- 1986-05-09 KR KR1019870700019A patent/KR930002887B1/ko not_active Expired - Lifetime
- 1986-05-09 WO PCT/DK1986/000051 patent/WO1986006743A1/en not_active Ceased
- 1986-05-09 CN CN86104387A patent/CN1008377B/zh not_active Expired
- 1986-05-09 IE IE123686A patent/IE59296B1/en not_active IP Right Cessation
- 1986-05-09 AT AT86106348T patent/ATE83264T1/de not_active IP Right Cessation
- 1986-05-09 ES ES554856A patent/ES8800346A1/es not_active Expired
- 1986-05-09 DE DE198686106348T patent/DE229866T1/de active Pending
1987
- 1987-01-09 DK DK198700097A patent/DK173445B1/da not_active IP Right Cessation
- 1987-08-26 ES ES557687A patent/ES8801946A1/es not_active Expired
1989
- 1989-06-23 AU AU36793/89A patent/AU3679389A/en not_active Abandoned

Also Published As

Publication number	Publication date
DK9787D0 (da)	1987-01-09
ES8801946A1 (es)	1988-03-01
ES557687A0 (es)	1988-03-01
IE861236L (en)	1986-11-10
FI870052L (fi)	1987-01-07
CN86104387A (zh)	1987-04-22
DK9787A (da)	1987-01-09
IE59296B1 (en)	1994-02-09
KR880700069A (ko)	1988-02-15
EP0229866B1 (en)	1992-12-09
FI870052A7 (fi)	1987-01-07
AU594062B2 (en)	1990-03-01
FI870052A0 (fi)	1987-01-07
EP0229866A1 (en)	1987-07-29
KR930002887B1 (ko)	1993-04-12
WO1986006743A1 (en)	1986-11-20
CA1327328C (en)	1994-03-01
IL78703A0 (en)	1986-08-31
ATE83264T1 (de)	1992-12-15
DE3687266D1 (de)	1993-01-21
CN1008377B (zh)	1990-06-13
IL78703A (en)	1993-02-21
ES8800346A1 (es)	1987-11-01
DE229866T1 (de)	1988-01-14
AU5910086A (en)	1986-12-04
AU3679389A (en)	1989-11-23
DE3687266T2 (de)	1993-04-15
ES554856A0 (es)	1987-11-01

Publication	Publication Date	Title
DK173445B1 (da)	2000-11-06	Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba
US5173418A (en)	1992-12-22	Production in Escherichia coli of extracellular Serratia spp. hydrolases
RU1838410C (ru)	1993-08-30	Способ получени альфа-амилазы
DK175738B1 (da)	2005-02-07	Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer
Figurski et al.	1976	Generation in vitro of deletions in the broad host range plasmid RK2 using phage Mu insertions and a restriction endonuclease
Kornacker et al.	1990	Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsielia pneumoniae UNF5023
JPH1014586A (ja)	1998-01-20	オリゴペプチド反復単位を有する大ポリペプチド
US4348478A (en)	1982-09-07	Method for preparation of a recombinant DNA phage
Kiliç et al.	1999	Streptococcal reporter gene-fusion vector for identification of in vivo expressed genes
CA1150169A (en)	1983-07-19	Broad host range small plasmid rings as cloning vehicles
JPS59501694A (ja)	1984-10-11	シグナルｄｎａ配列を含むｄｎａ断片、その製造方法およびそれにより形質転換された微生物
US6017730A (en)	2000-01-25	Method of limiting the survival of genetically engineered microorganisms in their environment
CN104152480A (zh)	2014-11-19	布鲁氏菌L7/L12、Omp31、Rsα、sodC免疫蛋白共表达载体构建和表达方法
US4801541A (en)	1989-01-31	Method of increasing the yield of a product by altering a microorganism
FR2559781A1 (fr)	1985-08-23	Nouveau vecteur plasmidique hybride de e. coli conferant le pouvoir de faire fermenter le saccharose et son procede de preparation
DK161105B (da)	1991-05-27	Bakteriophag samt fremgangsmaade til fremstilling deraf
EP0032238A2 (en)	1981-07-22	Recombinant bacteriophage for heterologous cloning of bacillus microorganisms and method for its production
DK164001B (da)	1992-04-27	Rekombinant-dna, der kan udtrykke staphylokinase og er afledt fra en temperat phag af staphylococcus aureus, fremgangsmaade til fremstilling af rekominant-dna samt fremgangsmaade til fremstilling af staphylokinase
DK175020B1 (da)	2004-04-26	Fremgangsåde til udtrykkelse og ekstracellulær udskillelse af proteiner i G(-)bakterie, rekombinant DNA-konstruktion og plasmidvektor kodende herfor, samt G(-)bakterie indeholdende disse
JPS62503074A (ja)	1987-12-10	不安定な遺伝性レプリコンの安定化方法
JP2604365B2 (ja)	1997-04-30	細菌酵素類
Barras et al.	1986	Export of Erwinia chrysanthemi (EC16) protease by Escherichia coli
Black et al.	1976	Head morphologies in bacteriophage T4 head and internal protein mutant infections
CN110305824A (zh)	2019-10-08	展示有机磷水解酶的枯草芽胞及其制备方法
JPS61141881A (ja)	1986-06-28	グラム陽性微生物由来の安定アルフア−アミラ−ゼ遺伝子を含有するグラム陰性微生物

Legal Events

Date	Code	Title	Description
2000-11-06	B1	Patent granted (law 1993)
2006-05-29	PUP	Patent expired