DK175496B1 - Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin - Google Patents

Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin Download PDF

Info

Publication number: DK175496B1
Authority: DK; Denmark
Prior art keywords: dna; hirudin; desulfatohirudin; solution; transformed
Prior art date: 1984-06-14

Application number

DK198502656A

Other languages

Danish (da)

English (en)

Other versions

DK265685D0 (da

DK265685A (da

Inventor

Markus Gerhard Gruetter

Manfred Liersch

Hans Rink

Walter Maerki

Bernd Meyhack

Original Assignee

Ucp Gen Pharma Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-06-14

Filing date

1985-06-13

Publication date

2004-11-08

1985-06-13 Application filed by Ucp Gen Pharma Ag filed Critical Ucp Gen Pharma Ag

1985-06-13 Publication of DK265685D0 publication Critical patent/DK265685D0/da

1985-12-15 Publication of DK265685A publication Critical patent/DK265685A/da

2004-11-08 Application granted granted Critical

2004-11-08 Publication of DK175496B1 publication Critical patent/DK175496B1/da

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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/38—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DK198502656A 1984-06-14 1985-06-13 Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin DK175496B1 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
CH288284		1984-06-14
CH288284		1984-06-14

Publications (3)

Publication Number	Publication Date
DK265685D0 DK265685D0 (da)	1985-06-13
DK265685A DK265685A (da)	1985-12-15
DK175496B1 true DK175496B1 (da)	2004-11-08

Family

ID=4243708

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198502656A DK175496B1 (da)	1984-06-14	1985-06-13	Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin

Country Status (23)

Country	Link
US (2)	US5422249A (fi)
EP (1)	EP0168342B1 (fi)
JP (1)	JPH0720434B2 (fi)
KR (1)	KR920009522B1 (fi)
AT (1)	ATE64956T1 (fi)
AU (1)	AU591061B2 (fi)
CA (1)	CA1341426C (fi)
CY (1)	CY1782A (fi)
DD (1)	DD240392A5 (fi)
DE (1)	DE3583361D1 (fi)
DK (1)	DK175496B1 (fi)
ES (3)	ES8708012A1 (fi)
FI (1)	FI90787C (fi)
GR (1)	GR851428B (fi)
HK (1)	HK11095A (fi)
HU (1)	HU202288B (fi)
IE (1)	IE57999B1 (fi)
IL (1)	IL75508A (fi)
NO (2)	NO177433C (fi)
NZ (1)	NZ212405A (fi)
PH (1)	PH26867A (fi)
PT (1)	PT80631B (fi)
ZA (1)	ZA854418B (fi)

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FR2607516B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
JPH0616716B2 (ja) *	1986-10-14	1994-03-09	塩野義製薬株式会社	酵母におけるヒトｐｓｔｉの製造法
FR2607517B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
US5256559A (en) *	1988-03-04	1993-10-26	Biogen, Inc.	Methods and compositions for inhibiting platelet aggregation
DE3819079A1 (de) *	1988-06-04	1989-12-07	Hoechst Ag	Hirudin-derivate mit verzoegerter wirkung
GB8817161D0 (en) *	1988-07-19	1988-08-24	Ciba Geigy Ag	Modified proteins
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US5112615A (en) *	1988-08-03	1992-05-12	New England Deaconess Hospital Corporation	Soluble hirudin conjugates
US5167960A (en) *	1988-08-03	1992-12-01	New England Deaconess Hospital Corporation	Hirudin-coated biocompatible substance
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
EP0380443B2 (en) *	1989-01-25	1999-06-30	Novartis AG	Monoclonal antibodies specific for thrombin hirudin
EP0385410B1 (en) *	1989-02-28	1996-10-02	Canon Kabushiki Kaisha	Partially double-stranded oligonucleotide and method for forming oligonucleotide
JPH037583A (ja) *	1989-02-28	1991-01-14	Canon Inc	部分的二本鎖オリゴヌクレオチド及びオリゴヌクレオチドの形成方法
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
GB9006400D0 (en)	1990-03-22	1990-05-23	Ciba Geigy Ag	Bacterial vectors
US6060451A (en) *	1990-06-15	2000-05-09	The National Research Council Of Canada	Thrombin inhibitors based on the amino acid sequence of hirudin
US5296352A (en) *	1990-10-02	1994-03-22	Ciba-Geigy Corporation	Monoclonal antibodies directed against complexes formed by thrombin and hirudin
US6514730B1 (en)	1991-03-21	2003-02-04	Consortium für elektrochemische Industrie GmbH	Secretion of hirudin derivatives
DE4209110A1 (de) *	1991-11-26	1993-05-27	Basf Ag	Neue thrombininhibitorische proteine aus landblutegeln
DE4140381A1 (de) *	1991-12-07	1993-06-09	Hoechst Ag, 6230 Frankfurt, De	Neue synthetische isohirudine mit verbesserter stabilitaet
HU214698B (hu) *	1992-04-09	1998-06-29	Gyógyszerkutató Intézet Kft.	Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
DE4404168A1 (de) *	1994-02-10	1995-08-17	Hoechst Ag	Hirudinderivate und Verfahren zu deren Herstellung
DE4421052A1 (de) *	1994-06-17	1995-12-21	Basf Ag	Neue Thrombininhibitoren, ihre Herstellung und Verwendung
US5643580A (en) *	1994-10-17	1997-07-01	Surface Genesis, Inc.	Biocompatible coating, medical device using the same and methods
SA96170106A (ar) *	1995-07-06	2005-12-03	أسترا أكتيبولاج	مشتقات حامض أميني جديدة
DE19529997C1 (de)	1995-08-16	1997-04-10	Hoechst Ag	Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE19543737A1 (de)	1995-11-24	1997-05-28	Hoechst Ag	Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) *	1995-11-28	1997-06-05	Hoechst Ag	Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
DE69625623T2 (de)	1996-01-31	2003-11-06	Sumitomo Bakelite Co. Ltd., Tokio/Tokyo	Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
US6623981B2 (en) *	1998-01-27	2003-09-23	Bristol-Myers Squibb Company	Detection of patients at risk for developing integrin antagonist/agonist mediated disease states
DE10149678A1 (de)	2001-10-09	2009-03-12	Novel Science International Gmbh	Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
US20070016969A1 (en) *	2002-01-18	2007-01-18	Animal Technology Institute Taiwan	Expression vector for hirudin and transformed cells and transgenic animals containing said vector
DE10301255A1 (de)	2003-01-15	2004-07-29	Cpi Creative Pharma International Gmbh	Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
JP3535151B1 (ja) *	2003-07-10	2004-06-07	株式会社ミナキアドバンス	生酵母菌を成分とする液剤の製造方法及びその液剤
US7795205B2 (en)	2004-04-12	2010-09-14	Canyon Pharmaceuticals, Inc.	Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
PT2242479E (pt) *	2007-12-28	2011-11-25	Baxter Int	Filtração de proteínas por contrapressão

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GR79124B (fi) *	1982-12-22	1984-10-02	Genentech Inc
WO1985000831A1 (en) *	1983-08-10	1985-02-28	Amgen	Microbial expression of insulin-like growth factor
DE3342139A1 (de) *	1983-11-22	1985-05-30	Ciba-Geigy Ag, Basel	Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
CA1341414C (fr) *	1984-03-27	2002-12-31	Paul Tolstoshev	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
FR2562088B1 (fr) *	1984-03-27	1987-11-13	Transgene Sa	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3438296A1 (de) *	1984-04-18	1985-11-07	Hoechst Ag, 6230 Frankfurt	Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
EP0209061B1 (de) *	1985-07-17	1994-01-12	Hoechst Aktiengesellschaft	Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel
DE3541856A1 (de) *	1985-11-27	1987-06-04	Hoechst Ag	Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
MY101203A (en) *	1985-12-12	1991-08-17	Ucp Gen Pharma Ag	Production of thrombin iinhibitors.
FR2645175B1 (fr) *	1989-03-31	1994-02-18	Transgene Sa	Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche

1985
- 1985-06-10 DE DE8585810268T patent/DE3583361D1/de not_active Expired - Lifetime
- 1985-06-10 AT AT85810268T patent/ATE64956T1/de not_active IP Right Cessation
- 1985-06-10 EP EP85810268A patent/EP0168342B1/de not_active Expired - Lifetime
- 1985-06-11 PH PH32401A patent/PH26867A/en unknown
- 1985-06-12 ZA ZA854418A patent/ZA854418B/xx unknown
- 1985-06-12 CA CA000483736A patent/CA1341426C/en not_active Expired - Fee Related
- 1985-06-12 DD DD85277301A patent/DD240392A5/de not_active IP Right Cessation
- 1985-06-12 GR GR851428A patent/GR851428B/el unknown
- 1985-06-12 PT PT80631A patent/PT80631B/pt unknown
- 1985-06-12 FI FI852342A patent/FI90787C/fi not_active IP Right Cessation
- 1985-06-13 NZ NZ212405A patent/NZ212405A/xx unknown
- 1985-06-13 ES ES544126A patent/ES8708012A1/es not_active Expired
- 1985-06-13 AU AU43655/85A patent/AU591061B2/en not_active Expired
- 1985-06-13 IL IL75508A patent/IL75508A/xx not_active IP Right Cessation
- 1985-06-13 NO NO852389A patent/NO177433C/no unknown
- 1985-06-13 IE IE1475/85A patent/IE57999B1/en not_active IP Right Cessation
- 1985-06-13 HU HU852340A patent/HU202288B/hu unknown
- 1985-06-13 JP JP60127266A patent/JPH0720434B2/ja not_active Expired - Lifetime
- 1985-06-13 DK DK198502656A patent/DK175496B1/da not_active IP Right Cessation
- 1985-06-14 KR KR1019850004195A patent/KR920009522B1/ko not_active Expired
1986
- 1986-10-01 ES ES557115A patent/ES8707765A1/es not_active Expired
- 1986-10-01 ES ES557116A patent/ES8707766A1/es not_active Expired
1993
- 1993-09-15 US US08/121,974 patent/US5422249A/en not_active Expired - Lifetime
1995
- 1995-01-26 HK HK11095A patent/HK11095A/xx not_active IP Right Cessation
- 1995-03-03 US US08/398,416 patent/US5728549A/en not_active Expired - Lifetime
- 1995-10-20 CY CY178295A patent/CY1782A/xx unknown
1999
- 1999-11-17 NO NO1999023C patent/NO1999023I1/no unknown

Also Published As

Publication number	Publication date
PH26867A (en)	1992-11-16
FI852342A0 (fi)	1985-06-12
GR851428B (fi)	1985-11-25
ES557115A0 (es)	1987-08-16
IL75508A0 (en)	1985-10-31
ES557116A0 (es)	1987-08-16
IL75508A (en)	1991-06-10
EP0168342B1 (de)	1991-07-03
FI90787B (fi)	1993-12-15
PT80631A (de)	1985-07-01
IE851475L (en)	1985-12-14
HUT38674A (en)	1986-06-30
KR860000381A (ko)	1986-01-28
DK265685D0 (da)	1985-06-13
NO1999023I1 (no)	1999-11-17
HU202288B (en)	1991-02-28
ES8707766A1 (es)	1987-08-16
AU591061B2 (en)	1989-11-30
DE3583361D1 (de)	1991-08-08
NZ212405A (en)	1989-01-06
CY1782A (en)	1995-10-20
HK11095A (en)	1995-02-03
NO852389L (no)	1985-12-16
ES8707765A1 (es)	1987-08-16
ZA854418B (en)	1986-02-26
FI852342L (fi)	1985-12-15
DK265685A (da)	1985-12-15
US5422249A (en)	1995-06-06
ES544126A0 (es)	1987-09-01
US5728549A (en)	1998-03-17
NO177433C (no)	1995-09-13
JPH0720434B2 (ja)	1995-03-08
FI90787C (fi)	1994-03-25
ATE64956T1 (de)	1991-07-15
CA1341426C (en)	2003-04-01
JPS6119492A (ja)	1986-01-28
KR920009522B1 (ko)	1992-10-17
DD240392A5 (de)	1986-10-29
NO177433B (no)	1995-06-06
AU4365585A (en)	1985-12-19
ES8708012A1 (es)	1987-09-01
IE57999B1 (en)	1993-06-02
EP0168342A1 (de)	1986-01-15
PT80631B (de)	1987-04-28

Publication	Publication Date	Title
DK175496B1 (da)	2004-11-08	Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin
KR960002874B1 (ko)	1996-02-27	재조합된 사람의 내피 세포 성장 인자의 제조방법
Fortkamp et al.	1986	Cloning and expression in Escherichia coli of a synthetic DNA for hirudin, the blood coagulation inhibitor in the leech
DK175182B1 (da)	2004-06-28	Mini-proinsulin, denne forbindelse til anvendelse som lægemiddel, lægemiddel indeholdende forbindelsen, anvendelse af forbindelsen til fremstilling af mono-Arg-insulin, fremgangsmåde til fremstilling af forbindelsen, DNA, der koder for forbindelsen......
US5702920A (en)	1997-12-30	DNAS encoding human macrophage migration inhibition factor related peptides
US5089400A (en)	1992-02-18	Polypeptides and process for the production thereof
HU209146B (en)	1994-03-28	Method for producing desulphato-hydrudine
US5869264A (en)	1999-02-09	Immunoassays for and immunopurification of interferon-induced human protein
US5026639A (en)	1991-06-25	Method to improve mRNA translation and use thereof for production of platelet factor-4
IE902426A1 (en)	1991-06-19	Novel cytokines
US20020142414A1 (en)	2002-10-03	Process for the preparation of protease inhibitors
EP0242329B1 (en)	1995-07-19	Monoclonal antibodies against interferon-induced human protein in pure form, and test kits containing these antibodies
US5198350A (en)	1993-03-30	Interferon-induced human protein in pure form, monoclonal antibodies thereto and test kits containing these antibodies
US6342373B1 (en)	2002-01-29	Process for preparing recombinant eglin, protease inhibitor
EP0035454A2 (en)	1981-09-09	Desacetylthymosin alpha 1, its process of preparation and its use
US6410272B1 (en)	2002-06-25	Production of thrombin inhibitors
EP0339285A2 (en)	1989-11-02	Recombinant endonexin II
CA2002765A1 (en)	1990-05-10	Gar transformylase inhibitor

Legal Events

Date	Code	Title	Description
1989-02-13	AHB	Application shelved due to non-payment
2005-07-04	PUP	Patent expired