[go: up one dir, main page]

DK1573058T3 - Fremgangsmåde til forbedring af proteinproduktion - Google Patents

Fremgangsmåde til forbedring af proteinproduktion

Info

Publication number
DK1573058T3
DK1573058T3 DK03779044.1T DK03779044T DK1573058T3 DK 1573058 T3 DK1573058 T3 DK 1573058T3 DK 03779044 T DK03779044 T DK 03779044T DK 1573058 T3 DK1573058 T3 DK 1573058T3
Authority
DK
Denmark
Prior art keywords
protein
sequence
cell
trap
protein expression
Prior art date
Application number
DK03779044.1T
Other languages
English (en)
Inventor
Blokland Henricus Johannes Maria Van
Arie Pieter Otte
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of DK1573058T3 publication Critical patent/DK1573058T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK03779044.1T 2002-12-18 2003-12-02 Fremgangsmåde til forbedring af proteinproduktion DK1573058T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02080347 2002-12-18
PCT/NL2003/000850 WO2004055215A1 (en) 2002-12-18 2003-12-02 A method for improving protein production

Publications (1)

Publication Number Publication Date
DK1573058T3 true DK1573058T3 (da) 2012-01-16

Family

ID=32524041

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03779044.1T DK1573058T3 (da) 2002-12-18 2003-12-02 Fremgangsmåde til forbedring af proteinproduktion

Country Status (9)

Country Link
US (2) US7413896B2 (da)
EP (1) EP1573058B1 (da)
AT (1) ATE524559T1 (da)
AU (1) AU2003285823B2 (da)
CA (1) CA2510179C (da)
DK (1) DK1573058T3 (da)
ES (1) ES2373549T3 (da)
NZ (1) NZ540471A (da)
WO (1) WO2004055215A1 (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1404872T3 (da) 2001-07-04 2007-02-19 Chromagenics Bv Fremgangsmåde til selektion af en DNA-sekvens med transkriptionsmodulerende aktivitet med en vektor indeholdende et element med en gentranskriptionsundertrykkende aktivitet
WO2003106674A2 (en) 2002-06-14 2003-12-24 Chromagenics B.V. Means and methods for regulating gene expression
WO2003106684A2 (en) 2002-06-14 2003-12-24 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
DK1573058T3 (da) 2002-12-18 2012-01-16 Chromagenics Bv Fremgangsmåde til forbedring af proteinproduktion
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
NZ553700A (en) 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
CA2581422C (en) * 2004-11-08 2012-05-22 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7566655B2 (en) * 2005-05-26 2009-07-28 Applied Materials, Inc. Integration process for fabricating stressed transistor structure
US7732342B2 (en) * 2005-05-26 2010-06-08 Applied Materials, Inc. Method to increase the compressive stress of PECVD silicon nitride films
SG164383A1 (en) 2005-07-21 2010-09-29 Abbott Lab Multiple gene expression including sorf constructs and methods with polyproteins, pro-proteins, and proteolysis
US20080293086A1 (en) * 2006-09-18 2008-11-27 Cobalt Technologies, Inc. A Delaware Corporation Real time monitoring of microbial enzymatic pathways
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
CL2009000879A1 (es) 2008-04-09 2010-05-07 Talfryn S A Metodo para fabricar un producto en un biorreactor que comprende cultivar microorganismos que son adaptados o mutagenizados para que exhiban por lo menos un 150% de tolerancia al producto; sistema para fabricar un producto; biorreactor.
CA2804912A1 (en) * 2009-06-26 2012-12-29 Cobalt Technologies, Inc. Integrated system and process for bioproduct production
EP2493923A1 (en) * 2009-10-30 2012-09-05 Abbott Laboratories Sorf constructs and multiple gene expression
ES2759910T3 (es) * 2013-08-21 2020-05-12 Curevac Ag Composición y vacuna para el tratamiento del cáncer de pulmón

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5658763A (en) * 1993-10-25 1997-08-19 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
US5972605A (en) 1994-07-07 1999-10-26 Geron Corporation Assays for regulators of mammalian telomerase expression
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
MXPA01000830A (es) 1998-07-21 2002-06-04 Cobra Therapeutics Ltd Un polinucleotido que comprende un elemento de abertura ubiculo de cromatina.
US6872524B1 (en) * 1998-08-14 2005-03-29 Chromagenics B.V. Method of detecting an expression-enhancing sequence
US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2000023606A1 (en) 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
EP1263950A2 (en) 2000-02-11 2002-12-11 Millennium Pharmaceuticals, Inc. Seven-transmembrane proteins/g-protein coupled receptors
US6586205B1 (en) 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
KR100996016B1 (ko) 2000-09-20 2010-11-22 밀리포어 코포레이션 폴리뉴클레오티드
US6787321B1 (en) * 2000-10-13 2004-09-07 The Regents Of The University Of California Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products
JP2004536572A (ja) * 2001-02-23 2004-12-09 セル・ジェネシス・インコーポレイテッド 新規ベクター構築物
DK1404872T3 (da) * 2001-07-04 2007-02-19 Chromagenics Bv Fremgangsmåde til selektion af en DNA-sekvens med transkriptionsmodulerende aktivitet med en vektor indeholdende et element med en gentranskriptionsundertrykkende aktivitet
EP1273666A1 (en) 2001-07-04 2003-01-08 Chromagenics B.V. Method of selecting a DNA sequence with transcription modulating activity using a vector comprising an element with a gene transcription repressing activity
ITMI20012110A1 (it) * 2001-10-12 2003-04-12 Keryos Spa Vettori multi-cistronici utilizzabili in protocolli di trsferimento genico
WO2003106684A2 (en) * 2002-06-14 2003-12-24 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
DK1573058T3 (da) 2002-12-18 2012-01-16 Chromagenics Bv Fremgangsmåde til forbedring af proteinproduktion
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
NZ553700A (en) * 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
CA2581422C (en) * 2004-11-08 2012-05-22 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1996705B1 (en) * 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof

Also Published As

Publication number Publication date
CA2510179A1 (en) 2004-07-01
US20090011468A1 (en) 2009-01-08
ATE524559T1 (de) 2011-09-15
ES2373549T3 (es) 2012-02-06
EP1573058B1 (en) 2011-09-14
US8173422B2 (en) 2012-05-08
AU2003285823B2 (en) 2008-06-19
US20060010506A1 (en) 2006-01-12
CA2510179C (en) 2013-04-23
AU2003285823A1 (en) 2004-07-09
NZ540471A (en) 2008-07-31
WO2004055215A1 (en) 2004-07-01
US7413896B2 (en) 2008-08-19
EP1573058A1 (en) 2005-09-14

Similar Documents

Publication Publication Date Title
DK1573058T3 (da) Fremgangsmåde til forbedring af proteinproduktion
FR2822164B1 (fr) Polypeptides derives des arn polymerases, et leurs utilisations
ATE374828T1 (de) Expressionsvektor zur antibiotikafreien expression
DK1062226T3 (da) Nukleinsyreligander med høj affinitet mod komplementsystemets C5-protein
SG156672A1 (en) Methods for refolding of recombinant antibodies
DE602004020321D1 (de) Endomannosidasen zur modifzierung der glykoproteine in eukaryoten
EP1350842A3 (de) Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität
WO2005118620A3 (en) Methods for preparing internally constraied peptides and peptidomimetics
ATE355382T1 (de) Verfahren zur fermentativen herstellung von l- threonine
ATE469223T1 (de) Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
DE602005009856D1 (de) Verfahren zur reinigung von fsh
DE60228847D1 (da)
ATE465241T1 (de) Verfahren zur fermentativen herstellung von proteinen
WO2005059149A3 (en) Improved protein production
ATE515516T1 (de) Pektin, verfahren zu dessen herstellung, azide eiweissnahrungsmittel mit der verwendung von pektin, sowie verfahren zur herstellung derselben
ATE469917T2 (de) Verfahren zur gewinnung von antikörpern
DE60219632D1 (de) Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon
DE60314132D1 (de) Methode zur produktion monoklonaler antikörper
DK1400589T3 (da) Fremgangsmåde til påvisning af prokaryotisk DNA
ATE341943T1 (de) Verfahren zur extraktion, reinigung und enzymatischen veränderung von soja 7s globulin alpha' untereinheit zur verwendung als hypocholesterol-mischer-wirkstoff
ATE445009T1 (de) Signalpeptid zur herstellung eines polypeptids
WO2005017237A3 (en) Apparatus and method for the selective assembly of protein
ATE486943T1 (de) Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner
WO2006002195A3 (en) Method of complexing a protein by the use of a dispersed system and proteins thereof
WO2005058362A3 (en) Method for opening tight junctions comprising the use of jam-1, claudin-4 and occludin protein antagonists as well as sirna molecules