DK0600965T3 - Primere til matrixafhængige, enzymatiske nukleinsyresynteser - Google Patents

Primere til matrixafhængige, enzymatiske nukleinsyresynteser

Info

Publication number: DK0600965T3
Authority: DK; Denmark
Prior art keywords: pct; nucleic acid; primers; enzymatic nucleic; dependent
Prior art date: 1991-08-28

Application number

DK92917597T

Other languages

English (en)

Inventor

Hartmut Seliger

Wojciech T Markiewicz

Gabriele Griger

Rudi Risch

Margit Klotz

Original Assignee

Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-08-28

Filing date

1992-08-22

Publication date

1998-09-23

1992-08-22 Application filed by Boehringer Mannheim Gmbh, Roche Diagnostics Gmbh filed Critical Boehringer Mannheim Gmbh

1998-09-23 Application granted granted Critical

1998-09-23 Publication of DK0600965T3 publication Critical patent/DK0600965T3/da

Links

108020004707 nucleic acids Proteins 0.000 title abstract 3
150000007523 nucleic acids Chemical class 0.000 title abstract 3
102000039446 nucleic acids Human genes 0.000 title abstract 3
238000003786 synthesis reaction Methods 0.000 title abstract 3
230000001419 dependent effect Effects 0.000 title abstract 2
230000002255 enzymatic effect Effects 0.000 title abstract 2
239000011159 matrix material Substances 0.000 title 1
238000003752 polymerase chain reaction Methods 0.000 abstract 2
108091034117 Oligonucleotide Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
238000004458 analytical method Methods 0.000 abstract 1
230000015572 biosynthetic process Effects 0.000 abstract 1
239000000969 carrier Substances 0.000 abstract 1
238000002372 labelling Methods 0.000 abstract 1
239000007791 liquid phase Substances 0.000 abstract 1
239000002243 precursor Substances 0.000 abstract 1
238000012163 sequencing technique Methods 0.000 abstract 1
239000007790 solid phase Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Engineering & Computer Science (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

DK92917597T 1991-08-28 1992-08-22 Primere til matrixafhængige, enzymatiske nukleinsyresynteser DK0600965T3 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE4128480		1991-08-28
PCT/EP1992/001932 WO1993005060A1 (de)	1991-08-28	1992-08-22	Funktionalisierte trägermaterialien für gleichzeitige synthese und direktmarkierung von oligonukleotiden als primer für matrizenabhängige enzymatische nukleinsäuresynthesen

Publications (1)

Publication Number	Publication Date
DK0600965T3 true DK0600965T3 (da)	1998-09-23

Family

ID=6439283

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK92917597T DK0600965T3 (da)	1991-08-28	1992-08-22	Primere til matrixafhængige, enzymatiske nukleinsyresynteser

Country Status (10)

Country	Link
US (1)	US5574141A (da)
EP (1)	EP0600965B1 (da)
JP (1)	JP2562862B2 (da)
KR (1)	KR0123559B1 (da)
AT (1)	ATE163308T1 (da)
CA (1)	CA2113478C (da)
DE (2)	DE59209203D1 (da)
DK (1)	DK0600965T3 (da)
ES (1)	ES2116342T3 (da)
WO (1)	WO1993005060A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE4418691A1 (de) *	1994-05-28	1996-02-22	Boehringer Mannheim Gmbh	3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer
CA2190304A1 (en) *	1995-12-15	1997-06-16	Elazar Rabbani	Property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses
US6291164B1 (en)	1996-11-22	2001-09-18	Invitrogen Corporation	Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6235483B1 (en) *	2000-01-31	2001-05-22	Agilent Technologies, Inc.	Methods and kits for indirect labeling of nucleic acids
JP4128453B2 (ja)	2001-03-19	2008-07-30	プレジデントアンドフェロウズオブハーバードカレッジ	新規分子機能の進化
EP1402024B1 (en)	2001-06-20	2007-08-22	Nuevolution A/S	Templated molecules and methods for using such molecules
JP4657919B2 (ja) *	2002-08-19	2011-03-23	プレジデントアンドフェローズオブハーバードカレッジ	進化する新しい分子機能
US8017323B2 (en) *	2003-03-26	2011-09-13	President And Fellows Of Harvard College	Free reactant use in nucleic acid-templated synthesis
WO2007011722A2 (en)	2005-07-15	2007-01-25	President And Fellows Of Harvard College	Reaction discovery system
RU2573915C2 (ru)	2009-09-16	2016-01-27	Дженентек, Инк.	Содержащие суперспираль и/или привязку белковые комплексы и их применение
CN102782134B (zh)	2009-12-29	2017-11-24	库尔纳公司	通过抑制核呼吸因子1(nrf1)的天然反义转录物而治疗nrf1相关疾病
AR080793A1 (es)	2010-03-26	2012-05-09	Roche Glycart Ag	Anticuerpos biespecificos
SG191153A1 (en)	2010-12-23	2013-07-31	Hoffmann La Roche	Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
JP6162044B2 (ja)	2010-12-23	2017-07-12	エフ．ホフマン−ラロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−ＬａＲｏｃｈｅＡｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ	結合剤
JP6046049B2 (ja)	2010-12-23	2016-12-14	エフ．ホフマン−ラロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−ＬａＲｏｃｈｅＡｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ	二価結合剤による翻訳後修飾されたポリペプチドの検出
KR20140127854A (ko)	2012-02-10	2014-11-04	제넨테크, 인크.	단일-쇄 항체 및 다른 이종다량체
JP6309002B2 (ja)	2012-06-27	2018-04-11	エフ．ホフマン−ラロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−ＬａＲｏｃｈｅＡｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ	標的に特異的に結合する少なくとも１つの結合実体を含む抗体Ｆｃ領域結合体を作製するための方法およびその使用
KR20150023889A (ko)	2012-06-27	2015-03-05	에프. 호프만-라 로슈 아게	2 개 이상의 상이한 결합 단위를 함유하는 맞춤-제작된 고도로 선별적이고 다중-특이적인 표적화 단위의 선별 및 제조 방법 및 이의 용도
WO2014021938A1 (en) *	2012-08-02	2014-02-06	The Board Of Trustees Of The Leland Stanford Junior University	Methods and apparatus for nucleic acid synthesis using oligo-templated polymerization
GB201408623D0 (en)	2014-05-15	2014-07-02	Santaris Pharma As	Oligomers and oligomer conjugates
ES2764111T3 (es)	2014-12-03	2020-06-02	Hoffmann La Roche	Anticuerpos multiespecíficos

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SU666183A1 (ru) *	1977-06-15	1979-06-05	Новосибирский институт органической химии СО АН СССР	Способ получени активных производных динуклеотидов
FR2519005B1 (fr) *	1981-12-29	1985-10-25	Pasteur Institut	Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
ES8706823A1 (es) *	1985-03-28	1987-06-16	Cetus Corp	Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
US4739044A (en) *	1985-06-13	1988-04-19	Amgen	Method for derivitization of polynucleotides
US4762779A (en) *	1985-06-13	1988-08-09	Amgen Inc.	Compositions and methods for functionalizing nucleic acids
SU1318600A1 (ru) *	1985-10-09	1987-06-23	Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии	Способ твердофазного синтеза олигонуклеотидов
US4868105A (en) *	1985-12-11	1989-09-19	Chiron Corporation	Solution phase nucleic acid sandwich assay
SU1439108A1 (ru) *	1986-05-11	1988-11-23	Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии	Способ получени носителей дл твердофазного синтеза олигонуклеотидов
DE3801987A1 (de) *	1988-01-23	1989-07-27	Boehringer Mannheim Gmbh	Traeger zur chemischen oder/und enzymatischen umsetzung von nukleinsaeuren oder nukleinsaeurefragmenten an festphasen
US5082934A (en) *	1989-04-05	1992-01-21	Naxcor	Coumarin derivatives for use as nucleotide crosslinking reagents
US5141813A (en) *	1989-08-28	1992-08-25	Clontech Laboratories, Inc.	Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
US5403709A (en) *	1992-10-06	1995-04-04	Hybridon, Inc.	Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages

1992
- 1992-08-22 CA CA002113478A patent/CA2113478C/en not_active Expired - Lifetime
- 1992-08-22 DE DE59209203T patent/DE59209203D1/de not_active Expired - Lifetime
- 1992-08-22 US US08/199,248 patent/US5574141A/en not_active Expired - Lifetime
- 1992-08-22 EP EP92917597A patent/EP0600965B1/de not_active Expired - Lifetime
- 1992-08-22 KR KR1019940700659A patent/KR0123559B1/ko not_active Expired - Lifetime
- 1992-08-22 DE DE4227882A patent/DE4227882A1/de not_active Withdrawn
- 1992-08-22 JP JP5504888A patent/JP2562862B2/ja not_active Expired - Lifetime
- 1992-08-22 DK DK92917597T patent/DK0600965T3/da active
- 1992-08-22 AT AT92917597T patent/ATE163308T1/de not_active IP Right Cessation
- 1992-08-22 WO PCT/EP1992/001932 patent/WO1993005060A1/de not_active Ceased
- 1992-08-22 ES ES92917597T patent/ES2116342T3/es not_active Expired - Lifetime

Also Published As

Publication number	Publication date
CA2113478A1 (en)	1993-03-18
EP0600965A1 (de)	1994-06-15
CA2113478C (en)	2001-06-12
ES2116342T3 (es)	1998-07-16
DE4227882A1 (de)	1993-04-01
JP2562862B2 (ja)	1996-12-11
JPH06506700A (ja)	1994-07-28
EP0600965B1 (de)	1998-02-18
ATE163308T1 (de)	1998-03-15
WO1993005060A1 (de)	1993-03-18
DE59209203D1 (de)	1998-03-26
KR0123559B1 (ko)	1997-11-28
US5574141A (en)	1996-11-12

Publication	Publication Date	Title
DK0600965T3 (da)	1998-09-23	Primere til matrixafhængige, enzymatiske nukleinsyresynteser
IL88923A0 (en)	1989-08-15	Gene encoding a thermostable dna polymerase from thermus aquaticus,said dna polymerase and its purification
DE69730926D1 (de)	2004-11-04	Modifizierte thermostabile DNA Polymerase
AU4300389A (en)	1990-04-18	Methods for dna sequencing with thermus aquaticus dna polymerase
EP0138357A3 (en)	1986-05-07	Labeled dna
NZ332753A (en)	1999-11-29	Artificial mismatch hybridization
DE69132477D1 (en)	2000-12-28	Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
DK2080813T3 (da)	2011-03-28	Nukleinsyreligander, som binder til og inhiberer DNA-polymeraser
ATE233816T1 (de)	2003-03-15	Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima
ATE198083T1 (de)	2000-12-15	Thermostable nukleinsäure polymerase
ES2186293T3 (es)	2003-05-01	Metodo para la amplificacion exponencial directa y secuenciacion de moleculas de dna y su aplicacion.
DE69419248D1 (de)	1999-07-29	Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung
DK0707659T3 (da)	1998-01-12	Identifikation af Salmonella ved hjælp af polymerasekædereaktion
DE60023480D1 (de)	2005-12-01	Amplifikation und sequenzierung von primer-paaren und deren verwendung
WO1998021362A3 (de)	1998-07-02	Stabilisierte wässrige nukleosidtriphosphat-lösungen
EP0257970A3 (en)	1988-12-21	Novel organosiloxane inhibitors for hydrosilation reactions and polyorganosiloxane compositions containing same
DE60024464D1 (de)	2006-01-05	Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
JPS5538322A (en)	1980-03-17	S6,5'-cyclopyrimidine nucleoside derivative and its preparation
IL112750A (en)	1997-06-10	Nucleic acid detection kit
JPS5538321A (en)	1980-03-17	S6,2'-cyclopyrimidine nucleoside derivative and its preparation