[go: up one dir, main page]

DE69940785D1 - Proteinexpressionsvektor und benutzung desselbigen - Google Patents

Proteinexpressionsvektor und benutzung desselbigen

Info

Publication number
DE69940785D1
DE69940785D1 DE69940785T DE69940785T DE69940785D1 DE 69940785 D1 DE69940785 D1 DE 69940785D1 DE 69940785 T DE69940785 T DE 69940785T DE 69940785 T DE69940785 T DE 69940785T DE 69940785 D1 DE69940785 D1 DE 69940785D1
Authority
DE
Germany
Prior art keywords
expression vector
nucleotide sequence
protein expression
downstream side
cloning site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69940785T
Other languages
English (en)
Inventor
Hidetoshi Uemura
Akira Okui
Katsuya Kominami
Nozomi Yamaguchi
Shinichi Mitsui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuso Pharmaceutical Industries Ltd
Original Assignee
Fuso Pharmaceutical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuso Pharmaceutical Industries Ltd filed Critical Fuso Pharmaceutical Industries Ltd
Application granted granted Critical
Publication of DE69940785D1 publication Critical patent/DE69940785D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69940785T 1998-11-20 1999-11-19 Proteinexpressionsvektor und benutzung desselbigen Expired - Lifetime DE69940785D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP33151598 1998-11-20
PCT/JP1999/006474 WO2000031284A1 (en) 1998-11-20 1999-11-19 Protein expression vector and utilization thereof

Publications (1)

Publication Number Publication Date
DE69940785D1 true DE69940785D1 (de) 2009-06-04

Family

ID=18244518

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69940785T Expired - Lifetime DE69940785D1 (de) 1998-11-20 1999-11-19 Proteinexpressionsvektor und benutzung desselbigen

Country Status (8)

Country Link
US (1) US7029909B1 (de)
EP (1) EP1132479B1 (de)
JP (1) JP4475814B2 (de)
AT (1) ATE429504T1 (de)
AU (1) AU765111B2 (de)
CA (1) CA2350226C (de)
DE (1) DE69940785D1 (de)
WO (1) WO2000031284A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7276605B2 (en) * 2001-02-01 2007-10-02 Roche Diagnostics Operations, Inc. Method for producing recombinant trypsin
WO2003038049A2 (en) 2001-10-29 2003-05-08 Renovis, Inc. Method for isolating cell-type specific mrnas
PL195457B1 (pl) * 2002-08-05 2007-09-28 Zaklady Farm Polpharma Sa Kaseta ekspresyjna, dwucistronowy wektor plazmidowy, środek farmaceutyczny oraz ich zastosowanie
JP2006345831A (ja) * 2005-06-20 2006-12-28 P-Lap:Kk Ｄｎａ、ベクター、形質転換体、及びａｐａタンパク質の製造方法
JP5130662B2 (ja) * 2006-06-07 2013-01-30 Ｊｎｃ株式会社 タンパク質の可溶性タンパク質としての製造方法
WO2008051383A2 (en) * 2006-10-19 2008-05-02 Amgen Inc. Use of alcohol co-solvents to improve pegylation reaction yields
US7820623B2 (en) 2006-10-25 2010-10-26 Amgen Inc. Conjugated toxin peptide therapeutic agents
AU2008262490B2 (en) 2007-05-22 2011-11-17 Amgen Inc. Compositions and methods for producing bioactive fusion proteins
US20110071049A1 (en) 2008-03-12 2011-03-24 Nathaniel Heintz Methods and compositions for translational profiling and molecular phenotyping
WO2010108154A2 (en) 2009-03-20 2010-09-23 Amgen Inc. Selective and potent peptide inhibitors of kv1.3
WO2011063277A1 (en) 2009-11-20 2011-05-26 Amgen Inc. Anti-orai1 antigen binding proteins and uses thereof
KR20130097747A (ko) 2010-08-10 2013-09-03 암젠 인크 표적 항체에 대한 중화 항체의 검출을 위한 이중 기능 시험관내 표적 결합 분석평가
EP2619226B1 (de) 2010-09-22 2018-09-12 Amgen Inc. Träger-immunglobuline und verwendungen davon
CA2819552C (en) 2010-12-09 2023-09-26 Institut Pasteur Mgmt-based method for obtaining high yield of recombinant protein expression
JP2014507136A (ja) 2011-01-18 2014-03-27 アムジエン・インコーポレーテツド ＮａＶ１．７ノックアウトマウスとそれらの使用
AU2012228990B2 (en) 2011-03-16 2017-04-06 Amgen Inc. Potent and selective inhibitors of Nav1.3 and Nav1.7
WO2013126813A1 (en) 2012-02-22 2013-08-29 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
WO2013177533A1 (en) * 2012-05-25 2013-11-28 California Institute Of Technology Expression of secreted and cell-surface polypeptides
UY35397A (es) 2013-03-12 2014-10-31 Amgen Inc INHIBIDORES POTENTES Y SELECTIVOS DE NaV1.7
US11078464B2 (en) 2013-08-30 2021-08-03 Amgen Inc. High titer recombinant AAV vector production in adherent and suspension cells
EP3155003B9 (de) 2014-06-13 2020-12-30 Whitehead Institute for Biomedical Research Amyloid-beta-expression-konstrukte und verwendungen davon
US10463716B2 (en) 2014-10-13 2019-11-05 University Of Maryland, Baltimore Methods for treating cardiovascular dysfunction and improving fluid homeostasis with Elabela peptide hormone
WO2016061240A1 (en) 2014-10-15 2016-04-21 Amgen Inc. Promoter and regulatory elements for improved expression of heterologous genes in host cells
KR101787485B1 (ko) * 2015-12-30 2017-11-15 재단법인 의약바이오컨버젼스연구단 재조합 picp 단백질 및 이에 특이적으로 결합하는 항체의 제조방법
JP6937309B2 (ja) 2016-01-27 2021-09-22 ジャスト−エヴォテック バイオロジックス、インコーポレイテッド ハイブリッドプロモーターおよびその使用
JP7116059B2 (ja) 2016-11-21 2022-08-09 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド アフリベルセプト製剤及びその使用
JP7097433B2 (ja) 2017-08-17 2022-07-07 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド 宿主細胞ガレクチンおよび他の夾雑物からグリコシル化タンパク質を精製する方法
CN111356471A (zh) 2017-11-20 2020-06-30 济世发展生物药业有限公司 包含赖氨酸盐作为张力调节剂的阿柏西普制剂及其用途
CA3124711A1 (en) 2019-02-15 2020-08-20 Just-Evotec Biologics, Inc. Automated biomanufacturing systems, facilities, and processes
EP4370647A1 (de) 2021-07-15 2024-05-22 Just-Evotec Biologics, Inc. Bidirektionales perfusionssystem mit tangentialer strömungsfiltration (tff)
JP2025506122A (ja) 2022-02-10 2025-03-07 アムジエン・インコーポレーテツド ハイスループット配列決定のための抗体タンパク質産物発現コンストラクト
CA3266599A1 (en) 2022-09-07 2024-11-07 Quantitative Biosciences, Inc. UNIQUE VARIABLE DOMAIN ANTIBODIES SPECIFIC TO FENTANYL AND USE IN A CONTINUOUS AGGLUTINATION TEST
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers
CN118580307B (zh) * 2024-05-21 2025-05-27 广东合鸣生物科技有限公司 一种具备紧致抗皱功效的Pro.AH8乙酰基六肽-8及其制备方法和应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3582965B2 (ja) * 1996-08-23 2004-10-27 株式会社イムノ・ジャパン 哺乳動物細胞用発現ベクター

Also Published As

Publication number Publication date
ATE429504T1 (de) 2009-05-15
EP1132479A1 (de) 2001-09-12
WO2000031284A1 (en) 2000-06-02
US7029909B1 (en) 2006-04-18
CA2350226C (en) 2012-04-24
EP1132479B1 (de) 2009-04-22
JP4475814B2 (ja) 2010-06-09
AU765111B2 (en) 2003-09-11
CA2350226A1 (en) 2000-06-02
AU1184000A (en) 2000-06-13
EP1132479A4 (de) 2005-08-10

Similar Documents

Publication Publication Date Title
DE69940785D1 (de) Proteinexpressionsvektor und benutzung desselbigen
BR0113864A (pt) ácidos nucléicos tipo kcp isolados e suas proteìnas codificadas
NO995901L (no) Vektor
BRPI9912663B1 (pt) vacina contra cáncer e polipeptìdeo.
DK0737207T3 (da) Inhibitorer for humant plasmin afledt af Kunitz-domæner
DE60141546D1 (de) Polypeptide enthaltend extrazellulären Domäne von IL-20RA und/oderr IL-20RB
ATE394484T1 (de) Smad 6 und dessen anwendungen
NO940736L (no) DNA sekvenser som koder for gelonin polypeptid
BR0316324A (pt) Polipeptìdeos e conjugados de interferon-alfa
WO1999003881A3 (fr) Polypeptide chimerique comprenant le fragment b de la toxine shiga et des peptides d'interet therapeutique
CY1106978T1 (el) Ενζυμα φωσφοδιεστερασης
DK1908772T3 (da) Polypeptider og polynucleinsyrer af phorphorymonas gingivalis
DE69934415D1 (de) Neuartiges collectin
DK0919621T3 (da) Human TAK1 og DNA der koder derfor
SE9602822D0 (sv) New receptor
WO2000000506A3 (en) Human proteins having hydrophobic domains and dnas encoding these proteins
NO981707L (no) Xylanase, oligonukleotidsekvens som koder for xylanase og anvendelse derav
IL136496A0 (en) Nucleotide sequences encoding the tomato light hypersensitive phenotype, encoded proteins and uses thereof
IT1286829B1 (it) Plasmidi contenenti due o piu' competitori disposti in successione e loro uso in tecniche di pcr-competitiva
FR2710113B1 (fr) Barreau magnétique courbe ou rectiligne, formé d'aimants disjoints.
NO20011961D0 (no) PRV-1-genet og anvendelsen av dette
Deutsch et al. Erratum: Interaction Physics of the Fast Ignitor Concept [Phys. Rev. Lett. 77, 2483 (1996)]
GB9604518D0 (en) Novel compounds
DE69943149D1 (en) Serinprotease bssp2
ITMI920320A1 (it) Composizione marcante-denaturante particolarmente adatta per marcare e denaturare bitumi combustibili o prodotti affini.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition