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DE60332774D1 - Verbesserte sso7-polymerase-konjugat-proteine - Google Patents

Verbesserte sso7-polymerase-konjugat-proteine

Info

Publication number
DE60332774D1
DE60332774D1 DE60332774T DE60332774T DE60332774D1 DE 60332774 D1 DE60332774 D1 DE 60332774D1 DE 60332774 T DE60332774 T DE 60332774T DE 60332774 T DE60332774 T DE 60332774T DE 60332774 D1 DE60332774 D1 DE 60332774D1
Authority
DE
Germany
Prior art keywords
improved
polymerase conjugate
conjugate proteins
sso7 polymerase
sso7
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60332774T
Other languages
English (en)
Inventor
Yan Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Application granted granted Critical
Publication of DE60332774D1 publication Critical patent/DE60332774D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60332774T 2002-10-23 2003-10-20 Verbesserte sso7-polymerase-konjugat-proteine Expired - Lifetime DE60332774D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/280,139 US7666645B2 (en) 2002-10-23 2002-10-23 Sso7-polymerase conjugate proteins
PCT/US2003/032954 WO2004037979A2 (en) 2002-10-23 2003-10-20 Improved sso7-polymerase conjugate proteins

Publications (1)

Publication Number Publication Date
DE60332774D1 true DE60332774D1 (de) 2010-07-08

Family

ID=32106848

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60332774T Expired - Lifetime DE60332774D1 (de) 2002-10-23 2003-10-20 Verbesserte sso7-polymerase-konjugat-proteine

Country Status (11)

Country Link
US (2) US7666645B2 (de)
EP (1) EP1660650B1 (de)
JP (2) JP4505331B2 (de)
CN (2) CN1720324B (de)
AT (1) ATE469211T1 (de)
AU (1) AU2003284265B2 (de)
CA (1) CA2502335C (de)
DE (1) DE60332774D1 (de)
DK (1) DK1660650T3 (de)
ES (1) ES2344892T3 (de)
WO (1) WO2004037979A2 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7560260B2 (en) 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US9040276B2 (en) 2005-07-15 2015-05-26 Agilent Technologies, Inc. DNA binding protein-polymerase chimeras
JP5051423B2 (ja) 2006-07-26 2012-10-17 アイシン精機株式会社 改変型の耐熱性ＲｅｃＡタンパク質、及び該タンパク質を用いた核酸増幅方法
EP1930342B1 (de) * 2006-12-04 2012-01-25 Institut Pasteur Als Gerüst verwendeter OB-Fold zur Entwicklung neuer spezifischer Bindemittel
WO2009070604A1 (en) * 2007-11-27 2009-06-04 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step rt-pcr
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
SG172925A1 (en) * 2009-01-08 2011-08-29 Bio Rad Laboratories Methods and compositions for improving efficiency of nucleic acids amplification reactions
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US8535925B2 (en) 2009-04-10 2013-09-17 Bio-Rad Laboratories, Inc. Modified DNase compositions and methods of use thereof
CA2779255A1 (en) * 2009-10-29 2011-05-19 Bio-Rad Laboratories, Inc. Rapid detection of mycoplasma contamination in cell culture samples
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
WO2012138416A1 (en) 2011-04-08 2012-10-11 Bio-Rad Laboratories, Inc. Pcr reaction mixtures with decreased non-specific activity
WO2012138417A1 (en) * 2011-04-08 2012-10-11 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugates with decreased non-specific activity
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
US20130260422A1 (en) * 2011-09-01 2013-10-03 New England Biolabs, Inc. Compositions and Methods Relating to Variant DNA Polymerases and Synthetic DNA Polymerases
EP2909319B1 (de) * 2012-10-16 2018-05-23 DNA Polymerase Technology, Inc. Hemmungsresistente polymerasen
CN104630205B (zh) * 2015-01-26 2018-01-16 江南大学 一种增强DNA聚合酶Dbh持续合成DNA能力的方法
CN104560909B (zh) * 2015-01-26 2017-09-29 江南大学 一种催化dna合成效率提高的dna聚合酶
WO2016134059A1 (en) 2015-02-17 2016-08-25 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
EP3436602B1 (de) 2016-03-31 2024-08-14 Genia Technologies, Inc. Nanoporeprotein-konjugate und deren verwendungen
JP7058228B2 (ja) * 2016-06-24 2022-04-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 繋ぎ止められたヌクレオシド三リン酸を用いた核酸合成および配列決定
CN107475216B (zh) * 2017-08-15 2022-06-14 北京擎科生物科技有限公司 重组型耐热dna聚合酶及其应用
EP3483180A1 (de) * 2017-11-14 2019-05-15 Affilogic Multispezifische moleküle
CN109988817B (zh) * 2017-12-30 2020-12-04 安诺优达基因科技(北京)有限公司 一种随机打断dna的方法
US11604186B2 (en) 2018-10-17 2023-03-14 Molecular Devices (Austria) GmbH Real time western blot assays utilizing fluorescence resonance energy transfer (FRET)
CN111826366B (zh) * 2020-06-05 2022-08-02 广州英赞生物科技有限公司 一种直扩型热启动dna聚合酶及其制备方法与应用
EP4095254A3 (de) 2021-05-27 2022-12-14 New England Biolabs, Inc. Fragmentierung von dna
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
EP4471162A4 (de) * 2022-01-26 2025-12-17 Seegene Inc Verfahren zum nachweis von zielnukleinsäuren in einer probe
WO2023232075A1 (zh) * 2022-06-02 2023-12-07 上海吉量医药工程有限公司 一种rna聚合酶融合蛋白及其应用
CN116218953B (zh) * 2023-01-03 2024-08-09 南京诺唯赞生物科技股份有限公司 一种连接核酸片段与接头的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972603A (en) 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes

Also Published As

Publication number Publication date
US7666645B2 (en) 2010-02-23
CN1720324B (zh) 2010-05-26
JP2010166918A (ja) 2010-08-05
US20100279360A1 (en) 2010-11-04
EP1660650B1 (de) 2010-05-26
US8445249B2 (en) 2013-05-21
US20040081963A1 (en) 2004-04-29
CN1720324A (zh) 2006-01-11
AU2003284265B2 (en) 2008-08-28
ATE469211T1 (de) 2010-06-15
WO2004037979A3 (en) 2005-05-06
AU2003284265A1 (en) 2004-05-13
JP2006503580A (ja) 2006-02-02
WO2004037979A2 (en) 2004-05-06
CA2502335A1 (en) 2004-05-06
CA2502335C (en) 2012-09-04
JP4505331B2 (ja) 2010-07-21
ES2344892T3 (es) 2010-09-09
EP1660650A2 (de) 2006-05-31
CN101875924A (zh) 2010-11-03
DK1660650T3 (da) 2010-09-06
EP1660650A4 (de) 2007-07-04

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