DE60327740D1 - Expressionsklonierungsverfahren in filamentösen pilzen - Google Patents
Expressionsklonierungsverfahren in filamentösen pilzenInfo
- Publication number
- DE60327740D1 DE60327740D1 DE60327740T DE60327740T DE60327740D1 DE 60327740 D1 DE60327740 D1 DE 60327740D1 DE 60327740 T DE60327740 T DE 60327740T DE 60327740 T DE60327740 T DE 60327740T DE 60327740 D1 DE60327740 D1 DE 60327740D1
- Authority
- DE
- Germany
- Prior art keywords
- expression cloning
- polypeptide
- library
- cloning method
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001400 expression cloning Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 230000002538 fungal effect Effects 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 238000012512 characterization method Methods 0.000 abstract 1
- 239000013599 cloning vector Substances 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 230000014621 translational initiation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preliminary Treatment Of Fibers (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200200256 | 2002-02-19 | ||
| PCT/DK2003/000106 WO2003070956A1 (en) | 2002-02-19 | 2003-02-18 | Expression cloning methods in filamentous fungi |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60327740D1 true DE60327740D1 (de) | 2009-07-09 |
Family
ID=27741065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60327740T Expired - Lifetime DE60327740D1 (de) | 2002-02-19 | 2003-02-18 | Expressionsklonierungsverfahren in filamentösen pilzen |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20050170350A1 (de) |
| EP (1) | EP1478755B1 (de) |
| AT (1) | ATE432355T1 (de) |
| AU (1) | AU2003206683A1 (de) |
| DE (1) | DE60327740D1 (de) |
| DK (1) | DK1478755T3 (de) |
| WO (1) | WO2003070956A1 (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005080559A1 (en) | 2004-02-25 | 2005-09-01 | Novozymes A/S | Fungall cell wall degrading enzyme |
| ATE517188T1 (de) * | 2007-05-09 | 2011-08-15 | Novozymes As | Expressions-cloning-verfahren zur auswahl von bibliotheksklonen zur herstellung eines polypeptids von interesse |
| WO2009104622A1 (ja) | 2008-02-18 | 2009-08-27 | 明治製菓株式会社 | 耐熱性カタラーゼ |
| DK2496694T3 (en) | 2009-11-06 | 2017-06-06 | Novozymes Inc | COMPOSITIONS FOR SACCHARIFYING CELLULOS MATERIAL |
| EP2683830A1 (de) | 2011-03-09 | 2014-01-15 | Novozymes A/S | Verfahren zur verstärkung der zellulolyse-verstärkenden aktivität eines polypeptids |
| WO2012122477A1 (en) | 2011-03-10 | 2012-09-13 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| CA2834513A1 (en) | 2011-04-28 | 2012-11-01 | Novozymes, Inc. | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| EP2702162B1 (de) | 2011-04-29 | 2020-02-26 | Novozymes, Inc. | Verfahren zur verstärkung des abbaus oder der umwandlung von cellulose-material |
| EP2782998B1 (de) | 2011-11-22 | 2018-01-10 | Novozymes Inc. | Polypeptide mit beta-xylosidase-aktivität und dafür codierende polynukleotide |
| AU2012342456B2 (en) | 2011-11-25 | 2016-11-24 | Novozymes A/S | Polypeptides having lysozyme activity and polynucleotides encoding same |
| CN104837993A (zh) | 2012-12-07 | 2015-08-12 | 诺维信公司 | 用于在丝状真菌中产生位点特异性突变的方法 |
| CN105164254B (zh) | 2013-03-08 | 2019-06-28 | 诺维信公司 | 纤维二糖水解酶变体和编码它们的多核苷酸 |
| EP2994529B1 (de) | 2013-05-10 | 2018-11-21 | Novozymes A/S | Polypeptide mit xylanaseaktivität und polynukleotide zur codierung davon |
| CN107002056A (zh) | 2014-09-05 | 2017-08-01 | 诺维信公司 | 碳水化合物结合模块变体以及编码它们的多核苷酸 |
| WO2016138167A2 (en) | 2015-02-24 | 2016-09-01 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| WO2018085370A1 (en) | 2016-11-02 | 2018-05-11 | Novozymes A/S | Processes for reducing production of primeverose during enzymatic saccharification of lignocellulosic material |
| AU2018319349B2 (en) | 2017-08-25 | 2024-02-29 | Novozymes A/S | Enzyme assisted crude palm oil extraction |
| CN113302293A (zh) | 2018-12-12 | 2021-08-24 | 诺维信公司 | 具有木聚糖酶活性的多肽以及编码这些多肽的多核苷酸 |
| CN119307498A (zh) * | 2021-02-23 | 2025-01-14 | 中国科学院天津工业生物技术研究所 | 一组酿酒酵母人工启动子突变体 |
| CN114875046B (zh) * | 2022-05-30 | 2023-07-18 | 福州大学 | 一种丝状真菌复制子 |
| CN114958899A (zh) * | 2022-05-30 | 2022-08-30 | 福州大学 | 一种能在黑曲霉细胞内自主复制的复制子 |
| CN119913118B (zh) * | 2025-04-03 | 2025-06-24 | 湖南工程学院 | 一种漆酶突变体及其在处理退浆废水中的应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2703598B2 (ja) * | 1987-09-04 | 1998-01-26 | ノボ―ノルディスク アクティーゼルスカブ | アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター |
| US5648267A (en) * | 1992-11-13 | 1997-07-15 | Idec Pharmaceuticals Corporation | Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same |
| US6107477A (en) * | 1996-09-26 | 2000-08-22 | Aurora Biosciences Corporation | Non-optimal Kozaks sequences |
| EP1124949B1 (de) * | 1998-10-26 | 2006-07-12 | Novozymes A/S | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen |
| AU2001234443A1 (en) * | 2000-01-13 | 2001-07-24 | Novozymes Biotech, Inc. | Methods for producing a polypeptide using a crippled translational initiator sequence |
| GB0001401D0 (en) * | 2000-01-22 | 2000-03-08 | Univ Edinburgh | Methods for amplifying genetic material and use thereof |
-
2003
- 2003-02-18 US US10/504,646 patent/US20050170350A1/en not_active Abandoned
- 2003-02-18 AT AT03704338T patent/ATE432355T1/de not_active IP Right Cessation
- 2003-02-18 DK DK03704338T patent/DK1478755T3/da active
- 2003-02-18 DE DE60327740T patent/DE60327740D1/de not_active Expired - Lifetime
- 2003-02-18 AU AU2003206683A patent/AU2003206683A1/en not_active Abandoned
- 2003-02-18 EP EP03704338A patent/EP1478755B1/de not_active Expired - Lifetime
- 2003-02-18 WO PCT/DK2003/000106 patent/WO2003070956A1/en not_active Ceased
-
2010
- 2010-11-09 US US12/942,518 patent/US20110059862A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003070956A1 (en) | 2003-08-28 |
| US20110059862A1 (en) | 2011-03-10 |
| EP1478755A1 (de) | 2004-11-24 |
| US20050170350A1 (en) | 2005-08-04 |
| AU2003206683A1 (en) | 2003-09-09 |
| EP1478755B1 (de) | 2009-05-27 |
| DK1478755T3 (da) | 2009-09-14 |
| ATE432355T1 (de) | 2009-06-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |