DE60326603D1 - Verfahren zur klassifizierung und zählung von leukozyten - Google Patents
Verfahren zur klassifizierung und zählung von leukozytenInfo
- Publication number
- DE60326603D1 DE60326603D1 DE60326603T DE60326603T DE60326603D1 DE 60326603 D1 DE60326603 D1 DE 60326603D1 DE 60326603 T DE60326603 T DE 60326603T DE 60326603 T DE60326603 T DE 60326603T DE 60326603 D1 DE60326603 D1 DE 60326603D1
- Authority
- DE
- Germany
- Prior art keywords
- leukocytes
- difference
- scattered light
- classifying
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000265 leukocyte Anatomy 0.000 title abstract 10
- 238000000034 method Methods 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 4
- 230000002159 abnormal effect Effects 0.000 abstract 3
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 230000002489 hematologic effect Effects 0.000 abstract 1
- 239000003219 hemolytic agent Substances 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1477—Multiparameters
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002183259 | 2002-06-24 | ||
| PCT/JP2003/007910 WO2004001408A1 (ja) | 2002-06-24 | 2003-06-23 | 白血球の分類計数方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60326603D1 true DE60326603D1 (de) | 2009-04-23 |
Family
ID=29996675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60326603T Expired - Lifetime DE60326603D1 (de) | 2002-06-24 | 2003-06-23 | Verfahren zur klassifizierung und zählung von leukozyten |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7625730B2 (de) |
| EP (1) | EP1542008B1 (de) |
| JP (1) | JP4324552B2 (de) |
| AT (1) | ATE425456T1 (de) |
| AU (1) | AU2003243945A1 (de) |
| DE (1) | DE60326603D1 (de) |
| WO (1) | WO2004001408A1 (de) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60326603D1 (de) * | 2002-06-24 | 2009-04-23 | Sysmex Corp | Verfahren zur klassifizierung und zählung von leukozyten |
| JP4509526B2 (ja) * | 2003-10-10 | 2010-07-21 | シスメックス株式会社 | 細菌分析装置および方法 |
| SE528697C2 (sv) | 2005-03-11 | 2007-01-30 | Hemocue Ab | Volymetrisk bestämning av antalet vita blodkroppar i ett blodprov |
| EP1710579B1 (de) * | 2005-03-30 | 2014-07-30 | Sysmex Corporation | Verfahren und Vorrichtung zum Zählen von Megakaryocyten |
| JP4745030B2 (ja) * | 2005-11-15 | 2011-08-10 | シスメックス株式会社 | 血液分析装置 |
| US7625757B2 (en) | 2006-01-27 | 2009-12-01 | Sysmex Corporation | Reagent for immature leukocyte analysis and reagent kit |
| JP4806342B2 (ja) * | 2006-01-27 | 2011-11-02 | シスメックス株式会社 | 幼若白血球分析用試薬及び試薬キット |
| JP4976038B2 (ja) * | 2006-03-29 | 2012-07-18 | シスメックス株式会社 | 血液学的試料の測定方法 |
| JP5032792B2 (ja) * | 2006-05-22 | 2012-09-26 | 浜松ホトニクス株式会社 | 細胞選別装置 |
| JP4922682B2 (ja) * | 2006-06-29 | 2012-04-25 | シスメックス株式会社 | 分析装置 |
| JP4806334B2 (ja) * | 2006-11-27 | 2011-11-02 | シスメックス株式会社 | 血液学的試料の測定方法および測定装置 |
| US11035823B2 (en) | 2009-03-17 | 2021-06-15 | Qiagen Sciences, Llc | Methods and devices for sequencing nucleic acids in smaller batches |
| US8481259B2 (en) | 2007-02-05 | 2013-07-09 | Intelligent Bio-Systems, Inc. | Methods and devices for sequencing nucleic acids in smaller batches |
| US11940413B2 (en) | 2007-02-05 | 2024-03-26 | IsoPlexis Corporation | Methods and devices for sequencing nucleic acids in smaller batches |
| WO2008097455A1 (en) * | 2007-02-05 | 2008-08-14 | Intelligent Bio-Systems, Inc. | Detection device and methods of use |
| WO2009001869A1 (ja) * | 2007-06-25 | 2008-12-31 | Sysmex Corporation | 幼若白血球の分析用試薬及び分析用試薬キット |
| CN101680879B (zh) * | 2007-06-25 | 2014-05-21 | 希森美康株式会社 | 幼稚白细胞的分析用试剂及分析用试剂盒 |
| CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
| WO2009057525A1 (ja) * | 2007-10-29 | 2009-05-07 | Sysmex Corporation | 細胞分析装置及び細胞分析方法 |
| CN101475754A (zh) * | 2008-01-04 | 2009-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料,组合物及在生物样品染色中的用途 |
| JP5627457B2 (ja) * | 2008-05-09 | 2014-11-19 | シスメックス株式会社 | 血液分析方法 |
| CN101602762B (zh) * | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
| CN101726579B (zh) | 2008-10-17 | 2014-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液检测试剂和方法 |
| US8367358B2 (en) * | 2008-12-17 | 2013-02-05 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Reagent, kit and method for differentiating and counting leukocytes |
| JP2010204086A (ja) * | 2009-02-09 | 2010-09-16 | Nippon Koden Corp | 細胞処理液の製造方法、細胞核dna量測定方法及びその測定に用いるキット |
| JP5452058B2 (ja) * | 2009-03-31 | 2014-03-26 | シスメックス株式会社 | 血液分析装置 |
| CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
| GB201015569D0 (en) * | 2010-09-16 | 2010-10-27 | Medical Res Council | Blood assay for prions |
| JP2012088336A (ja) * | 2012-01-23 | 2012-05-10 | Sysmex Corp | 骨髄芽球と血小板凝集との弁別方法及び弁別装置 |
| JP5416232B2 (ja) * | 2012-01-23 | 2014-02-12 | シスメックス株式会社 | 血液学的試料の測定装置 |
| KR101885417B1 (ko) * | 2013-02-28 | 2018-08-03 | 시스멕스 가부시키가이샤 | 뇨 검체 분석 장치 및 뇨 검체 분석 방법 |
| JP6225085B2 (ja) * | 2013-08-30 | 2017-11-01 | シスメックス株式会社 | 検体分析方法および検体分析装置 |
| CN103745210B (zh) * | 2014-01-28 | 2018-02-06 | 爱威科技股份有限公司 | 一种白细胞分类方法及装置 |
| JP6317976B2 (ja) * | 2014-03-31 | 2018-04-25 | シスメックス株式会社 | 尿検体分析方法及び尿検体分析装置 |
| JP6238856B2 (ja) | 2014-08-25 | 2017-11-29 | シスメックス株式会社 | 尿中異型細胞の分析方法、尿分析装置および体液中異型細胞の分析方法 |
| EP3943912B1 (de) * | 2015-02-12 | 2025-08-20 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Zellanalysator und teilchensortierungsverfahren und -vorrichtung |
| JP6680492B2 (ja) * | 2015-09-11 | 2020-04-15 | シスメックス株式会社 | 細胞分析装置および細胞分析方法 |
| EP3258274B1 (de) | 2016-06-17 | 2019-11-06 | Sysmex Corporation | Verfahren zur steuerung eines blutanalysators zur plättchenmessung |
| WO2018232589A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种血小板聚集识别的方法、装置和细胞分析仪 |
| EP3789764A4 (de) * | 2018-04-28 | 2021-06-23 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Bluttestverfahren und blutanalysesystem |
| WO2020133285A1 (zh) * | 2018-12-28 | 2020-07-02 | 深圳迈瑞生物医疗电子股份有限公司 | 一种血液细胞参数修正方法、血液样本检测仪和存储介质 |
| CN115885166B (zh) * | 2020-09-24 | 2025-10-28 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪、样本分析方法以及计算机可读存储介质 |
| CN120476300A (zh) | 2022-12-05 | 2025-08-12 | 贝克曼库尔特有限公司 | 多通道血液学流动系统 |
| WO2025145095A1 (en) | 2023-12-29 | 2025-07-03 | Beckman Coulter, Inc. | Customized sample handling in biological analysis |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07113632B2 (ja) | 1991-04-22 | 1995-12-06 | 株式会社日立製作所 | 白血球分析方法 |
| JP3070968B2 (ja) * | 1991-05-14 | 2000-07-31 | シスメックス株式会社 | 尿中の細胞分析用試薬及び方法 |
| JP2565844B2 (ja) | 1992-02-07 | 1996-12-18 | アボツト・ラボラトリーズ | 細胞溶解処理条件下で異種細胞集団を正確に計数し,感受性に関する格付けを行う方法 |
| JP3301646B2 (ja) | 1993-03-19 | 2002-07-15 | シスメックス株式会社 | 幼若細胞測定用試薬 |
| DE69535413T2 (de) * | 1994-10-20 | 2007-11-29 | Sysmex Corp. | Reagenz und Verfahren zur Analyse fester Bestandteile im Harn |
| JP4042925B2 (ja) | 1996-11-20 | 2008-02-06 | シスメックス株式会社 | 幼若白血球の分類計数法 |
| JP3783808B2 (ja) * | 1997-05-19 | 2006-06-07 | シスメックス株式会社 | 白血球分類計数用試薬 |
| WO2000003033A1 (en) * | 1998-07-08 | 2000-01-20 | The Regents Of The University Of California | Use of fluorescent reagents in identification of cancerous cells and activated lymphocytes |
| US6386246B2 (en) * | 1999-11-17 | 2002-05-14 | Marconi Commerce Systems Inc. | Vapor flow and hydrocarbon concentration sensor for improved vapor recovery in fuel dispensers |
| JP2002148261A (ja) * | 2000-11-09 | 2002-05-22 | Sysmex Corp | 異常細胞分類計数方法 |
| JP4212827B2 (ja) * | 2002-05-16 | 2009-01-21 | シスメックス株式会社 | 骨髄液有核細胞自動分析方法 |
| DE60326603D1 (de) * | 2002-06-24 | 2009-04-23 | Sysmex Corp | Verfahren zur klassifizierung und zählung von leukozyten |
-
2003
- 2003-06-23 DE DE60326603T patent/DE60326603D1/de not_active Expired - Lifetime
- 2003-06-23 AU AU2003243945A patent/AU2003243945A1/en not_active Abandoned
- 2003-06-23 US US10/517,422 patent/US7625730B2/en not_active Expired - Lifetime
- 2003-06-23 JP JP2004515543A patent/JP4324552B2/ja not_active Expired - Lifetime
- 2003-06-23 EP EP03733538A patent/EP1542008B1/de not_active Expired - Lifetime
- 2003-06-23 AT AT03733538T patent/ATE425456T1/de not_active IP Right Cessation
- 2003-06-23 WO PCT/JP2003/007910 patent/WO2004001408A1/ja not_active Ceased
-
2007
- 2007-10-29 US US11/926,918 patent/US7923229B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1542008B1 (de) | 2009-03-11 |
| JPWO2004001408A1 (ja) | 2005-10-20 |
| US20050202400A1 (en) | 2005-09-15 |
| WO2004001408A1 (ja) | 2003-12-31 |
| ATE425456T1 (de) | 2009-03-15 |
| US20080176274A1 (en) | 2008-07-24 |
| US7923229B2 (en) | 2011-04-12 |
| JP4324552B2 (ja) | 2009-09-02 |
| EP1542008A1 (de) | 2005-06-15 |
| EP1542008A4 (de) | 2006-07-12 |
| AU2003243945A1 (en) | 2004-01-06 |
| US7625730B2 (en) | 2009-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60326603D1 (de) | Verfahren zur klassifizierung und zählung von leukozyten | |
| DVORAK et al. | Trypanosoma cruzi: Flow Cytometric Analysis. I. Analysis of Total DNA/Organism by Means of Mithramycin‐Induced Fluorescence 1, 2 | |
| US4745285A (en) | Multi-color fluorescence analysis with single wavelength excitation | |
| Olson et al. | An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions | |
| DK0545315T3 (da) | Reagenssammensætninger og deres anvendelse ved identificeringen og karakteriseringen af reticulocytter i helblod. | |
| CA1191079A (en) | Metachromatic dye sorption means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes | |
| US5958776A (en) | Method for classifying and counting immature leukocytes | |
| Shapiro et al. | Combined blood cell counting and classification with fluorochrome stains and flow instrumentation. | |
| ATE305050T1 (de) | Verfahren zur färbung, zum nachweis und zählen von bakterien, und ein verdünner für bakterienfarbstoff | |
| US9995736B2 (en) | Preparation and use of nucleated red blood cell simulating particles and hematology control mixtures | |
| CN1149337A (zh) | 网织红细胞的检测 | |
| DK0545314T3 (da) | Reagenssammensætninger og deres anvendelse ved identificeringen og karakteriseringen af reticulocytter i helblod. | |
| US20090105963A1 (en) | Methods for Flow Cytometry Analyses of Un-Lysed Cells from Biological Fluids | |
| CN1323395A (zh) | 鉴别网状细胞的试剂组合物和方法 | |
| JPH04184168A (ja) | フローサイトメトリーによる白血球の分類方法 | |
| US6271035B1 (en) | Methods and compositions for rapid staining of nucleic acids in whole cells | |
| DE60208341T2 (de) | Multidimensionale Analyse von Leukozyten | |
| CN103398935B (zh) | 一种用于白细胞分类计数的方法以及试剂盒 | |
| Gilman-Sachs | Flow cytometry | |
| EP0259833B1 (de) | Reagenz und Verfahren zum Klassifizieren von Leukozyten durch Durchfluss-Zytometrie | |
| CN1183559A (zh) | 早幼白细胞的分类计数法 | |
| JP4354982B2 (ja) | 白血球の分類計数方法 | |
| RU2023106140A (ru) | Способ классификации лейкоцитов на субпопуляции | |
| KR19980042627A (ko) | 유약백혈구의 분류계수법 | |
| Jensen et al. | [27] Flow cytometry: Rapid isolation and analysis of single cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |