DE60323631D1 - Verfahren zur reinigung einer rhizomucor aspartat-protease - Google Patents
Verfahren zur reinigung einer rhizomucor aspartat-proteaseInfo
- Publication number
- DE60323631D1 DE60323631D1 DE60323631T DE60323631T DE60323631D1 DE 60323631 D1 DE60323631 D1 DE 60323631D1 DE 60323631 T DE60323631 T DE 60323631T DE 60323631 T DE60323631 T DE 60323631T DE 60323631 D1 DE60323631 D1 DE 60323631D1
- Authority
- DE
- Germany
- Prior art keywords
- microbial protease
- separation medium
- rhizomucor
- cleaning
- aspartate protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 101000898643 Candida albicans Vacuolar aspartic protease Proteins 0.000 title 1
- 101000898783 Candida tropicalis Candidapepsin Proteins 0.000 title 1
- 101000898784 Cryphonectria parasitica Endothiapepsin Proteins 0.000 title 1
- 241000235402 Rhizomucor Species 0.000 title 1
- 101000933133 Rhizopus niveus Rhizopuspepsin-1 Proteins 0.000 title 1
- 101000910082 Rhizopus niveus Rhizopuspepsin-2 Proteins 0.000 title 1
- 101000910079 Rhizopus niveus Rhizopuspepsin-3 Proteins 0.000 title 1
- 101000910086 Rhizopus niveus Rhizopuspepsin-4 Proteins 0.000 title 1
- 101000910088 Rhizopus niveus Rhizopuspepsin-5 Proteins 0.000 title 1
- 101000898773 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Saccharopepsin Proteins 0.000 title 1
- 238000004140 cleaning Methods 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 abstract 5
- 239000004365 Protease Substances 0.000 abstract 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 5
- 230000000813 microbial effect Effects 0.000 abstract 5
- 238000000926 separation method Methods 0.000 abstract 4
- 239000003446 ligand Substances 0.000 abstract 2
- 239000011159 matrix material Substances 0.000 abstract 2
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 125000001183 hydrocarbyl group Chemical group 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02100578 | 2002-05-29 | ||
| PCT/EP2003/005724 WO2003100048A1 (en) | 2002-05-29 | 2003-05-28 | Method for the purification of microbial protease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60323631D1 true DE60323631D1 (de) | 2008-10-30 |
Family
ID=29558402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60323631T Expired - Lifetime DE60323631D1 (de) | 2002-05-29 | 2003-05-28 | Verfahren zur reinigung einer rhizomucor aspartat-protease |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7670821B2 (de) |
| EP (1) | EP1507858B1 (de) |
| AT (1) | ATE408681T1 (de) |
| AU (1) | AU2003232845A1 (de) |
| DE (1) | DE60323631D1 (de) |
| DK (1) | DK1507858T3 (de) |
| WO (1) | WO2003100048A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE0004808D0 (sv) | 2000-12-20 | 2000-12-20 | Apbiotech Ab | Method for the purification of an enzyme |
| WO2007081911A2 (en) * | 2006-01-05 | 2007-07-19 | Amgen Inc. | Systems and methods for packing chromatography columns |
| DK2333056T3 (da) | 2006-04-13 | 2014-08-04 | Dsm Ip Assets Bv | Væskeformig sammensætning, der omfatter en asparaginprotease |
| CN102383555A (zh) * | 2011-10-11 | 2012-03-21 | 常熟市华夏建筑节能材料有限公司 | 一种节能天花板 |
| KR101743094B1 (ko) * | 2015-05-13 | 2017-06-07 | 대한민국 | 신규 리조무코르 푸실루스 ｍ2559 균주 및 이의 용도 |
| WO2017089613A1 (en) | 2015-11-26 | 2017-06-01 | Csk Food Enrichment B.V. | Liquid composition comprising an aspartic protease having improved enzyme stability |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE280333C (de) | ||||
| US2683682A (en) | 1950-02-06 | 1954-07-13 | Research Corp | Differential inactivation of enzymes |
| DK110031A (de) | 1965-12-02 | |||
| US4086139A (en) * | 1976-04-09 | 1978-04-25 | Gb Fermentation Industries Inc. | Differential inactivation of amylase in amylase-protease mixtures |
| US4721673A (en) * | 1983-09-01 | 1988-01-26 | Genex Corporation | Recovery and activation process for microbially produced calf prochymosin |
| US4743551A (en) | 1984-11-05 | 1988-05-10 | Miles Inc. | Purification of microbial rennet from Mucor miehei |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| DD280333A1 (de) * | 1989-03-02 | 1990-07-04 | Adw Ddr | Verfahren zur gewinnung von milchfaellenden enzymen |
| US5139943A (en) * | 1989-06-13 | 1992-08-18 | Genencor International, Inc. | Processes for the recovery of microbially produced chymosin |
| DK165090D0 (da) | 1990-07-09 | 1990-07-09 | Kem En Tec As | Konglomererede partikler |
| SE9101149D0 (sv) | 1991-04-17 | 1991-04-17 | Pharmacia Lkb Biotech | Beads for down stream processing |
| AU653279B2 (en) * | 1991-12-30 | 1994-09-22 | Sanofi | Novel 2-saccharinylmethyl heterocyclic carboxylates useful as proteolytic enzyme inhibitors and compositions and method of use thereof |
| DE4323913A1 (de) | 1993-04-03 | 1995-01-19 | Merck Patent Gmbh | Trennmaterialien für die hydrophobe Chromatographie |
| DK47493D0 (da) | 1993-04-27 | 1993-04-27 | Novo Nordisk As | Fremgangsmaade til fremstilling af ost |
| US20040214157A1 (en) | 1994-06-29 | 2004-10-28 | Simon C. Burton | Chromatographic resins and methods for using same |
| US5652348A (en) * | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| FR2737892B1 (fr) * | 1995-08-17 | 1997-10-24 | Fournier Ind & Sante | Nouveaux composes de benzenesulfonamide, procede de preparation et utilisation en therapeutique |
| AU1092297A (en) | 1995-12-07 | 1997-06-27 | Novo Nordisk A/S | Selective inactivation of enzyme activities |
| SE9700383D0 (sv) | 1997-02-04 | 1997-02-04 | Pharmacia Biotech Ab | An adsorption/separation method and a medium for adsorption/separation |
| AU7349298A (en) * | 1997-06-23 | 1999-01-04 | Pfizer Inc. | Prostaglandin agonists |
| ATE346856T1 (de) * | 2000-02-08 | 2006-12-15 | Upfront Chromatography As | Neue harze und ihre verwendung zur rückgewinnung von proteinen oder peptiden |
| SE0004808D0 (sv) * | 2000-12-20 | 2000-12-20 | Apbiotech Ab | Method for the purification of an enzyme |
-
2003
- 2003-05-28 DE DE60323631T patent/DE60323631D1/de not_active Expired - Lifetime
- 2003-05-28 AU AU2003232845A patent/AU2003232845A1/en not_active Abandoned
- 2003-05-28 DK DK03755152T patent/DK1507858T3/da active
- 2003-05-28 AT AT03755152T patent/ATE408681T1/de not_active IP Right Cessation
- 2003-05-28 WO PCT/EP2003/005724 patent/WO2003100048A1/en not_active Ceased
- 2003-05-28 EP EP03755152A patent/EP1507858B1/de not_active Expired - Lifetime
- 2003-05-28 US US10/515,791 patent/US7670821B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| DK1507858T3 (da) | 2009-02-02 |
| WO2003100048A1 (en) | 2003-12-04 |
| ATE408681T1 (de) | 2008-10-15 |
| EP1507858B1 (de) | 2008-09-17 |
| AU2003232845A1 (en) | 2003-12-12 |
| US20050153418A1 (en) | 2005-07-14 |
| US7670821B2 (en) | 2010-03-02 |
| EP1507858A1 (de) | 2005-02-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |