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DE60321901D1 - Verbesserte bacillus-wirtszelle - Google Patents

Verbesserte bacillus-wirtszelle

Info

Publication number
DE60321901D1
DE60321901D1 DE60321901T DE60321901T DE60321901D1 DE 60321901 D1 DE60321901 D1 DE 60321901D1 DE 60321901 T DE60321901 T DE 60321901T DE 60321901 T DE60321901 T DE 60321901T DE 60321901 D1 DE60321901 D1 DE 60321901D1
Authority
DE
Germany
Prior art keywords
host cell
bacillus host
parent
improved bacillus
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60321901T
Other languages
English (en)
Inventor
Jens Toenne Andersen
Steen Troels Joergensen
Michael Dolbjerg Rasmussen
Peter Bjarke Olsen
Ib Groth Clausen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE60321901D1 publication Critical patent/DE60321901D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)
DE60321901T 2002-04-10 2003-03-25 Verbesserte bacillus-wirtszelle Expired - Lifetime DE60321901D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200534 2002-04-10
PCT/DK2003/000198 WO2003093453A2 (en) 2002-04-10 2003-03-25 Bacillus licheniformis mutant host cell

Publications (1)

Publication Number Publication Date
DE60321901D1 true DE60321901D1 (de) 2008-08-14

Family

ID=29286036

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60321901T Expired - Lifetime DE60321901D1 (de) 2002-04-10 2003-03-25 Verbesserte bacillus-wirtszelle

Country Status (8)

Country Link
US (3) US7521204B2 (de)
EP (2) EP1497429B1 (de)
CN (1) CN1659283A (de)
AT (2) ATE399869T1 (de)
AU (1) AU2003214034A1 (de)
DE (1) DE60321901D1 (de)
DK (2) DK1995319T3 (de)
WO (1) WO2003093453A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2359381T5 (es) 2002-05-14 2014-07-10 Novozymes A/S Variantes de pectato liasa
AU2013202671B2 (en) * 2003-07-02 2016-03-03 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
CN108486086A (zh) * 2003-07-02 2018-09-04 维莱尼姆公司 葡聚糖酶，编码它们的核酸以及制备和使用它们的方法
EP2216640A1 (de) * 2004-01-09 2010-08-11 Novozymes, Inc. Bacillus licheniformis Chromosom
EP2305821A3 (de) * 2004-02-13 2011-04-13 Novozymes A/S Proteasevarianten
DK1766002T3 (en) 2004-06-21 2015-12-07 Novozymes As Stable maintenance of multiple copies of the at least two ORF in the same orientation
EP1612267A1 (de) * 2004-07-02 2006-01-04 GBF Gesellschaft für Biotechnologische Forschung mbH Zellulasen aus Rumen
DE602006015444D1 (de) * 2005-03-22 2010-08-26 Novozymes As Polypeptide und nukleinsäuren, die diese codieren
CN101541968B (zh) * 2006-11-30 2013-11-13 诺维信公司 重组宿主细胞中的脱氧核糖核酸酶表达
EP2091554A2 (de) * 2006-12-22 2009-08-26 DSMIP Assets B.V. Verwendung von polypeptiden als coccidiostatikum und/oder histomonostatikum
EP2516636A1 (de) * 2009-12-21 2012-10-31 Novozymes Inc. Verfahren zur herstellung von heterologen polypeptiden bei mutierenden bakterienzellen mit thiol-disulfid-oxidoreduktasemangel
WO2018007435A1 (en) 2016-07-05 2018-01-11 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
CN106467903B (zh) * 2016-09-18 2019-10-08 齐鲁工业大学 一种适用于制浆过程的地衣芽孢杆菌工程菌及其构建方法
CN106893736B (zh) * 2017-01-24 2020-07-07 湖北大学 一种提高地衣芽胞杆菌外源蛋白分泌水平的方法
WO2019157061A2 (en) * 2018-02-06 2019-08-15 Novozymes Bioag A/S Methods of protecting a plant from fungal pests
US20240052000A1 (en) 2020-12-15 2024-02-15 Novozymes A/S Mutated host cells with reduced cell motility on
MX2023015460A (es) 2021-06-24 2024-01-18 Basf Se Celula huesped de bacillus mejorada con proteina rema/remb alterada.
MX2023015406A (es) 2021-06-24 2024-02-20 Basf Se Celula huesped de bacillus licheniformis para la produccion de un compuesto de interes con pureza aumentada.
EP4387982A2 (de) * 2021-08-20 2024-06-26 Danisco US Inc. Verfahren und zusammensetzungen zur verbesserten proteinproduktion in bacillus-zellen
PE20251535A1 (es) 2022-05-14 2025-06-05 Novozymes As Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2003078A1 (en) * 1988-11-18 1990-05-18 Alan Sloma Protease deletion
WO1991002803A1 (en) 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
US5294542A (en) 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
ATE248223T1 (de) 1991-11-14 2003-09-15 Novozymes As Ein bacillus-promotor, der von dem alpha-amylase- promotor einer variante des bacillus licheniformis abstammt
DK153992D0 (da) 1992-12-22 1992-12-22 Novo Nordisk As Metode
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
FI964808A0 (fi) 1994-06-03 1996-12-02 Novo Nordisk Biotech Inc Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot
WO1996023073A1 (en) 1995-01-23 1996-08-01 Novo Nordisk A/S Dna integration by transposition
JP2001503249A (ja) 1996-08-23 2001-03-13 ルーダル イェンセン，ペテル 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター
GB9708452D0 (en) 1997-04-26 1997-06-18 Univ Newcastle Improved prokaryotic expression of protein
US6100063A (en) 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
CN1160448C (zh) 1998-02-12 2004-08-04 诺维信公司 含有两拷贝以不同方向转录之基因的原核细胞
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
NZ527414A (en) 1998-03-04 2005-07-29 Genencor Int Modified forms of pullulanase
AU2001262063A1 (en) 2000-05-24 2001-12-03 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
WO2002000907A1 (en) * 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
EP1355931A2 (de) * 2000-10-06 2003-10-29 Novozymes Biotech, Inc. Methoden zur darstellung multipler genexpression

Also Published As

Publication number Publication date
AU2003214034A1 (en) 2003-11-17
ATE522614T1 (de) 2011-09-15
US20090176290A1 (en) 2009-07-09
EP1995319A1 (de) 2008-11-26
CN1659283A (zh) 2005-08-24
US7521204B2 (en) 2009-04-21
EP1995319B1 (de) 2011-08-31
US20090203074A1 (en) 2009-08-13
EP1497429B1 (de) 2008-07-02
WO2003093453A2 (en) 2003-11-13
DK1497429T3 (da) 2008-11-03
AU2003214034A8 (en) 2003-11-17
US20050244922A1 (en) 2005-11-03
DK1995319T3 (da) 2011-12-05
EP1497429A2 (de) 2005-01-19
WO2003093453A3 (en) 2004-05-13
US8911969B2 (en) 2014-12-16
ATE399869T1 (de) 2008-07-15

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