DE60317314D1 - Verfahren und gerät für die identifikation und zuordnung von biomolekülen - Google Patents
Verfahren und gerät für die identifikation und zuordnung von biomolekülenInfo
- Publication number
- DE60317314D1 DE60317314D1 DE60317314T DE60317314T DE60317314D1 DE 60317314 D1 DE60317314 D1 DE 60317314D1 DE 60317314 T DE60317314 T DE 60317314T DE 60317314 T DE60317314 T DE 60317314T DE 60317314 D1 DE60317314 D1 DE 60317314D1
- Authority
- DE
- Germany
- Prior art keywords
- membrane
- digestion
- electroblotted
- polypeptides
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012528 membrane Substances 0.000 abstract 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 5
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 230000029087 digestion Effects 0.000 abstract 3
- 239000002131 composite material Substances 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 102000007079 Peptide Fragments Human genes 0.000 abstract 1
- 108010033276 Peptide Fragments Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 102000004142 Trypsin Human genes 0.000 abstract 1
- 108090000631 Trypsin Proteins 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 238000004949 mass spectrometry Methods 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 abstract 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000012588 trypsin Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Discharge Of Articles From Conveyors (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36366302P | 2002-03-12 | 2002-03-12 | |
| US363663P | 2002-03-12 | ||
| US10/327,342 US7166441B2 (en) | 2002-03-12 | 2002-12-20 | Method and apparatus for the identification and quantification of biomolecules |
| US327342 | 2002-12-20 | ||
| PCT/US2003/006615 WO2003078452A1 (en) | 2002-03-12 | 2003-03-04 | Method and apparatus for the identification and qualification of biomolecules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60317314D1 true DE60317314D1 (de) | 2007-12-20 |
| DE60317314T2 DE60317314T2 (de) | 2008-09-04 |
Family
ID=28044867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60317314T Expired - Lifetime DE60317314T2 (de) | 2002-03-12 | 2003-03-04 | Verfahren und gerät für die identifikation und zuordnung von biomolekülen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7166441B2 (de) |
| EP (1) | EP1490394B1 (de) |
| JP (1) | JP4291161B2 (de) |
| AT (1) | ATE377756T1 (de) |
| DE (1) | DE60317314T2 (de) |
| WO (1) | WO2003078452A1 (de) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2243230A1 (en) * | 1998-07-15 | 2000-01-15 | Aled Edwards | A device and method for the determination of protein domain boundaries |
| WO2002014867A2 (en) * | 2000-08-11 | 2002-02-21 | Agilix Corporation | Ultra-sensitive detection systems |
| US7244702B2 (en) * | 2003-04-23 | 2007-07-17 | Nzedegwu Robert Olisa, III | Purified Cys moiety for iron reductase, and method for obtaining and using same |
| EP1676292A4 (de) * | 2003-10-10 | 2009-01-07 | Protein Discovery Inc | Verfahren und einrichtungen zum konzentrieren und reinigen von analyten für die chemische analyse unter verwendung der matrixunterstützten laserdesorptions-/-ionisations-(maldi-)massenspektrometrie (ms) |
| US7227066B1 (en) * | 2004-04-21 | 2007-06-05 | Nanosolar, Inc. | Polycrystalline optoelectronic devices based on templating technique |
| JP4317083B2 (ja) * | 2004-06-04 | 2009-08-19 | 株式会社日立ハイテクノロジーズ | 質量分析方法及び質量分析システム |
| JP2006078192A (ja) * | 2004-09-07 | 2006-03-23 | Shimadzu Corp | 生体高分子の質量分析法 |
| EP1815007A4 (de) | 2004-11-15 | 2011-10-19 | Univ North Dakota | Verfahren zum einbau eines einzelnen sauerstoffatoms in verdaute peptide unter verwendung von peptidasen |
| EP1866055A4 (de) * | 2005-04-05 | 2010-08-18 | Protein Discovery Inc | Verbesserte verfahren und vorrichtung zur konzentrierung und fraktionierung von analyten für chemische analysen mit matrixgestützter laserdesorptions-/ionisations-massenspektrometrie (maldi-ms) |
| WO2007075325A2 (en) * | 2005-12-13 | 2007-07-05 | Bio-Rad Laboratories, Inc. | Composite membrane to capture analyte transfers from gels |
| JP2007309673A (ja) * | 2006-05-16 | 2007-11-29 | Shionogi & Co Ltd | 微量タンパク質の同定を可能にするｍａｌｄｉ型質量分析装置用サンプルプレート |
| US8580534B2 (en) * | 2006-06-30 | 2013-11-12 | The University Of North Dakota | Method for incorporation of two oxygen atoms into digested peptides using peptidases |
| WO2008154523A2 (en) * | 2007-06-08 | 2008-12-18 | Protein Discovery, Inc. | Improved methods and devices for concentration and fractionation of analytes for chemical analysis including matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms) |
| US8075755B2 (en) * | 2007-12-13 | 2011-12-13 | Bio-Rad Laboratories, Inc. | Polymeric sorbent sheets as ion reservoirs for electroblotting |
| AR073295A1 (es) | 2008-09-16 | 2010-10-28 | Genentech Inc | Metodos para tratar la esclerosis multiple progresiva. articulo de fabricacion. |
| US20100213060A1 (en) * | 2009-02-23 | 2010-08-26 | Bio-Rad Laboratories, Inc. | Aqueous transfer buffer |
| EP2425216B1 (de) | 2009-04-27 | 2019-10-23 | Expedeon Holdings Ltd. | Programmierbare elektrophoretische kerbenfiltersysteme und verfahren dafür |
| US8325999B2 (en) | 2009-06-08 | 2012-12-04 | Microsoft Corporation | Assisted face recognition tagging |
| US8715476B2 (en) | 2009-12-11 | 2014-05-06 | Bio-Rad Laboratories, Inc. | Instrument for independent electrotransfer in multiple cassettes |
| CN101793865A (zh) * | 2010-03-29 | 2010-08-04 | 内蒙古蒙牛乳业（集团）股份有限公司 | 一种检测人乳中人血清白蛋白含量的方法 |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| EP3792635B1 (de) | 2012-03-30 | 2025-03-19 | BD Kiestra B.V. | Verfahren zur automatischen herstellung einer suspension einer probe von mikroorganismen |
| DE102012011647B4 (de) * | 2012-06-08 | 2020-07-02 | Bruker Daltonik Gmbh | Analyse von Mikroben aus Mikrokolonien mittels MALDI-Massenspektrometrie |
| DE102012011648B4 (de) * | 2012-06-08 | 2018-06-14 | Bruker Daltonik Gmbh | Analyse von Mikroben aus Mikrokolonien mittels MALDI-Massenspektrometrie |
| ES3019910T3 (en) | 2012-10-17 | 2025-05-21 | 10X Genomics Sweden Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CN103969321B (zh) * | 2013-01-24 | 2016-04-20 | 复旦大学 | 基于固定化酶原位高效酶解法的蛋白鉴定及质谱成像的方法 |
| CN118345148A (zh) | 2013-06-25 | 2024-07-16 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| TWI518316B (zh) * | 2014-04-01 | 2016-01-21 | 財團法人工業技術研究院 | 光學測讀系統及使用該系統之生化檢測方法 |
| WO2016143223A1 (ja) * | 2015-03-09 | 2016-09-15 | 株式会社島津製作所 | 外部環境変化に対する耐性が向上した固定化プロテアーゼ |
| ES2955488T3 (es) | 2015-04-10 | 2023-12-01 | Spatial Transcriptomics Ab | Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos |
| US10996227B2 (en) * | 2015-06-09 | 2021-05-04 | Vanderbilt University | Pre-coated surfaces for imaging biomolecules |
| CN105547967B (zh) * | 2016-01-28 | 2019-04-02 | 成都理工大学 | 裂隙介质系统渗透张量室内测定装置 |
| WO2019200166A1 (en) * | 2018-04-11 | 2019-10-17 | The Trustees Of Indiana University | Cartridges, systems and methods for mass spectrometry |
| CN113767177B (zh) | 2018-12-10 | 2025-01-14 | 10X基因组学有限公司 | 生成用于空间分析的捕获探针 |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| EP4164796A4 (de) | 2020-06-10 | 2024-03-06 | 10x Genomics, Inc. | Flüssigkeitsabgabeverfahren |
| CN111929132B (zh) * | 2020-08-07 | 2023-05-02 | 中央民族大学 | 一种交变电场耦合高频振动辅助基质喷涂方法 |
| CN116194590A (zh) | 2020-09-18 | 2023-05-30 | 10X基因组学有限公司 | 样品处理设备和图像配准方法 |
| AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| EP4305195A2 (de) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse |
| EP4441711A1 (de) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5625184A (en) | 1995-05-19 | 1997-04-29 | Perseptive Biosystems, Inc. | Time-of-flight mass spectrometry analysis of biomolecules |
| US5808300A (en) | 1996-05-10 | 1998-09-15 | Board Of Regents, The University Of Texas System | Method and apparatus for imaging biological samples with MALDI MS |
| US6348688B1 (en) | 1998-02-06 | 2002-02-19 | Perseptive Biosystems | Tandem time-of-flight mass spectrometer with delayed extraction and method for use |
| US6221626B1 (en) * | 1998-06-30 | 2001-04-24 | University Of Geneva | Kit for electroblotting polypeptides separated on an electrophoresis gel |
| US6441140B1 (en) | 1998-09-04 | 2002-08-27 | Cell Signaling Technology, Inc. | Production of motif-specific and context-independent antibodies using peptide libraries as antigens |
| US6787330B1 (en) | 1999-01-28 | 2004-09-07 | University Of Geneva | Method and kit for identifying or characterizing polypeptides |
| US20020012920A1 (en) * | 2000-11-20 | 2002-01-31 | Kevin Gardner | Method and kit for proteomic identification |
| US7045296B2 (en) * | 2001-05-08 | 2006-05-16 | Applera Corporation | Process for analyzing protein samples |
-
2002
- 2002-12-20 US US10/327,342 patent/US7166441B2/en not_active Expired - Lifetime
-
2003
- 2003-03-04 EP EP03713891A patent/EP1490394B1/de not_active Expired - Lifetime
- 2003-03-04 JP JP2003576456A patent/JP4291161B2/ja not_active Expired - Lifetime
- 2003-03-04 DE DE60317314T patent/DE60317314T2/de not_active Expired - Lifetime
- 2003-03-04 WO PCT/US2003/006615 patent/WO2003078452A1/en not_active Ceased
- 2003-03-04 AT AT03713891T patent/ATE377756T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP4291161B2 (ja) | 2009-07-08 |
| US7166441B2 (en) | 2007-01-23 |
| DE60317314T2 (de) | 2008-09-04 |
| ATE377756T1 (de) | 2007-11-15 |
| JP2005534284A (ja) | 2005-11-17 |
| EP1490394A4 (de) | 2006-05-17 |
| EP1490394B1 (de) | 2007-11-07 |
| EP1490394A1 (de) | 2004-12-29 |
| WO2003078452A1 (en) | 2003-09-25 |
| US20030175844A1 (en) | 2003-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US |