[go: up one dir, main page]

DE602006003108D1 - Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin - Google Patents

Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin

Info

Publication number
DE602006003108D1
DE602006003108D1 DE602006003108T DE602006003108T DE602006003108D1 DE 602006003108 D1 DE602006003108 D1 DE 602006003108D1 DE 602006003108 T DE602006003108 T DE 602006003108T DE 602006003108 T DE602006003108 T DE 602006003108T DE 602006003108 D1 DE602006003108 D1 DE 602006003108D1
Authority
DE
Germany
Prior art keywords
arginine
yield
improving
gel filtration
protein purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006003108T
Other languages
English (en)
Inventor
Daisuke Ejima
Ryosuke Yumioka
Tsutomu Arakawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of DE602006003108D1 publication Critical patent/DE602006003108D1/de
Anticipated expiration legal-status Critical
Active legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5412—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Endocrinology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
DE602006003108T 2005-03-03 2006-02-23 Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin Active DE602006003108D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US65773505P 2005-03-03 2005-03-03

Publications (1)

Publication Number Publication Date
DE602006003108D1 true DE602006003108D1 (de) 2008-11-27

Family

ID=36406515

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006003108T Active DE602006003108D1 (de) 2005-03-03 2006-02-23 Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin

Country Status (4)

Country Link
US (1) US7501495B2 (de)
EP (1) EP1698637B1 (de)
JP (1) JP4941882B2 (de)
DE (1) DE602006003108D1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
ATE497973T1 (de) * 2005-12-22 2011-02-15 Genentech Inc Rekombinante produktion heparinbindender proteine
SG162834A1 (en) * 2006-07-14 2010-07-29 Genentech Inc Refolding of recombinant proteins
JP5505853B2 (ja) * 2007-02-13 2014-05-28 味の素株式会社 微酸性アルギニンを添加剤とするウイルス不活化法
CN102089319B (zh) * 2008-05-08 2013-11-13 味之素株式会社 蛋白质的重折叠方法
JPWO2010071208A1 (ja) * 2008-12-19 2012-05-31 武田薬品工業株式会社 抗体の精製方法
PT2513134T (pt) 2009-12-18 2017-12-14 Novartis Ag Solução de lavagem e método para cromatografia de afinidade
PT2714714T (pt) 2011-06-01 2018-10-22 Novartis Ag Solução de lavagem e método para cromatografia de afinidade
DK2739641T4 (da) * 2011-08-02 2020-06-15 Baxalta GmbH Systemer og fremgangsmåder til at øge proteinudbytte fra rekombinante fremstillingsfremgangsmåder
JP6956942B2 (ja) * 2017-04-28 2021-11-02 味の素株式会社 ゲル濾過クロマトグラフィーにおけるタンパク質の回収率を高める方法
CN116056775B (zh) * 2020-09-16 2025-12-02 沃特世科技公司 在尺寸排阻色谱流动相中使用低浓度氨基酸的改善尺寸排阻色谱法
WO2022061026A1 (en) 2020-09-16 2022-03-24 Waters Technologies Corporation A sorbent used to improve chromatographic separations in size exclusion chromatography via reduced secondary interactions
KR102284800B1 (ko) * 2020-12-30 2021-08-03 주식회사 하플사이언스 재조합 세포외 기질 단백질의 생산을 위한 동물세포 배양용 배지 조성물 및 이를 이용한 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6489292B1 (en) * 1998-11-18 2002-12-03 Novo Nordisk A/S Stable aqueous insulin preparations without phenol and cresol
US7223848B2 (en) * 2001-06-05 2007-05-29 Genetics Institute, Llc Methods for purifying Fc-containing proteins
PL1610820T5 (pl) * 2003-04-04 2014-01-31 Genentech Inc Preparaty zawierające wysokoskoncentrowane przeciwciała i białka
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody

Also Published As

Publication number Publication date
JP4941882B2 (ja) 2012-05-30
US7501495B2 (en) 2009-03-10
EP1698637B1 (de) 2008-10-15
US20060199948A1 (en) 2006-09-07
JP2006242957A (ja) 2006-09-14
EP1698637A1 (de) 2006-09-06

Similar Documents

Publication Publication Date Title
DK1577319T3 (da) Proteinoprensning med præfiltrering
ZA200710330B (en) Methods of purifying FC region containing proteins
DE602005013775D1 (de) Verbesserte methode zur transformation von sojabohnen
IL199753A0 (en) Process for the purification of fc-containing proteins
PT2061803T (pt) Processo de purificação de proteinas contendo fc
EP1748077A4 (de) Verfahren zur proteinherstellung
EP2226331A4 (de) Verfahren zur reinigung von proteinen
DE602006009981D1 (de) Methode und Vorrichtung zur Fahrunterstützung
DK2061897T3 (da) Fed-batch-fermenteringsproces med høj celledensitet til fremstilling af rekombinant protein
DK2046819T3 (da) Fremgangsmåder til forøgelse af secernering af polypeptider med biologisk aktivitet
EP1856291A4 (de) Nukleinsäuren und proteine sowie verfahren zu ihrer herstellung und verwendung
DK1941026T3 (da) Fremgangsmåder til fremstilling af protein under anvendelse af anti-senescensforbindelser
DE602006003108D1 (de) Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
ATE442898T1 (de) Filtrationsverfahren
DK1989189T3 (da) Adsorbenter til proteinoprensning
DE602004005438D1 (de) Methode zur überwachung des nervensystems
ZA200900837B (en) Process for the purification of FC-containing proteins
EP1960416A4 (de) Verfahren zur aufreinigung von chenodesoxycholsäure
DE602006013109D1 (de) Markerprotein zur verwendung bei der diagnose von bauchspeicheldrüsenkrebs
DE602004011674D1 (de) Promotormoleküle zur verwendung in pflanzen
EP1870457A4 (de) Dipeptidkristall und verfahren zur herstellung davon
DE602004023969D1 (de) Methode zur herstellung von quervernetzten protein kristallen
ATE552335T1 (de) An das wachstum in suspension angepasste vero- zellinie, verfahren zur gewinnung davon und virale impfstoffe
DE502006008473D1 (de) Tetrahydrochinoline zur verwendung als modulatoren des mitotischen motor- protein eg5
ATE476403T1 (de) Verfahren zur herstellung und aufreinigung von n- propylbromid

Legal Events

Date Code Title Description
8364 No opposition during term of opposition