DE602004026229D1 - ENZYMATIC REMOVAL OF DIGLYCERIDES FROM EDIBLE OILS - Google Patents
ENZYMATIC REMOVAL OF DIGLYCERIDES FROM EDIBLE OILSInfo
- Publication number
- DE602004026229D1 DE602004026229D1 DE602004026229T DE602004026229T DE602004026229D1 DE 602004026229 D1 DE602004026229 D1 DE 602004026229D1 DE 602004026229 T DE602004026229 T DE 602004026229T DE 602004026229 T DE602004026229 T DE 602004026229T DE 602004026229 D1 DE602004026229 D1 DE 602004026229D1
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- variant
- amino acid
- optionally
- acyltransferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000008157 edible vegetable oil Substances 0.000 title 1
- 230000002255 enzymatic effect Effects 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 7
- 108090000790 Enzymes Proteins 0.000 abstract 7
- 102000057234 Acyl transferases Human genes 0.000 abstract 5
- 108700016155 Acyl transferases Proteins 0.000 abstract 5
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 abstract 4
- 229930186217 Glycolipid Natural products 0.000 abstract 4
- 125000000539 amino acid group Chemical group 0.000 abstract 3
- 239000000758 substrate Substances 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 102000004357 Transferases Human genes 0.000 abstract 1
- 108090000992 Transferases Proteins 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 150000002632 lipids Chemical class 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 150000003904 phospholipids Chemical class 0.000 abstract 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Fats And Perfumes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0330016.7A GB0330016D0 (en) | 2003-12-24 | 2003-12-24 | Method |
| PCT/IB2004/000655 WO2004064537A2 (en) | 2003-01-17 | 2004-01-15 | Method for the in situ production of an emulsifier in foodstuff |
| GBGB0416023.0A GB0416023D0 (en) | 2003-12-24 | 2004-07-16 | Method |
| US10/898,775 US7718408B2 (en) | 2003-12-24 | 2004-07-26 | Method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602004026229D1 true DE602004026229D1 (en) | 2010-05-06 |
Family
ID=30776521
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004026229T Expired - Lifetime DE602004026229D1 (en) | 2003-12-24 | 2004-12-23 | ENZYMATIC REMOVAL OF DIGLYCERIDES FROM EDIBLE OILS |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JP2010142243A (en) |
| CN (2) | CN103275944B (en) |
| AT (1) | ATE462011T1 (en) |
| DE (1) | DE602004026229D1 (en) |
| DK (1) | DK1704236T3 (en) |
| GB (3) | GB0330016D0 (en) |
| NZ (1) | NZ547085A (en) |
| RU (2) | RU2377307C2 (en) |
| ZA (1) | ZA200605158B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958800B (en) * | 2022-06-24 | 2023-08-25 | 北京脉道生物药品制造有限公司 | Taq DNA polymerase mutant capable of tolerating blood or blood product inhibition and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756328A (en) * | 1995-12-20 | 1998-05-26 | Cornell Research Foundation, Inc. | Acyltransferase and cDNA encoding acyltransferase |
| EP0977869B2 (en) * | 1997-04-09 | 2008-11-12 | Danisco A/S | Lipase and use of same for improving doughs and baked products |
| DE69904941T3 (en) * | 1998-07-21 | 2008-01-31 | Danisco A/S | FOOD |
| RU2235775C2 (en) * | 1998-11-27 | 2004-09-10 | Новозимс А/С | Method for preparing lipolytic enzyme variant and lipolytic enzyme (variants) |
| DE50115482D1 (en) * | 2001-07-11 | 2010-06-24 | Cognis Ip Man Gmbh | Lipase / acyltransferase from Candida parapsilosis |
| MXPA06001719A (en) * | 2003-08-11 | 2006-05-19 | Codexis Inc | Improved glucose dehydrogenase polypeptides and related polynucleotides. |
-
2003
- 2003-12-24 GB GBGB0330016.7A patent/GB0330016D0/en not_active Ceased
-
2004
- 2004-07-16 GB GBGB0415999.2A patent/GB0415999D0/en not_active Ceased
- 2004-07-16 GB GBGB0416023.0A patent/GB0416023D0/en not_active Ceased
- 2004-12-23 CN CN201310150130.9A patent/CN103275944B/en not_active Expired - Fee Related
- 2004-12-23 DE DE602004026229T patent/DE602004026229D1/en not_active Expired - Lifetime
- 2004-12-23 CN CN200910206577A patent/CN101676390A/en active Pending
- 2004-12-23 NZ NZ547085A patent/NZ547085A/en not_active IP Right Cessation
- 2004-12-23 AT AT07017066T patent/ATE462011T1/en not_active IP Right Cessation
- 2004-12-23 RU RU2006126654/13A patent/RU2377307C2/en not_active IP Right Cessation
- 2004-12-23 DK DK04806537.9T patent/DK1704236T3/en active
- 2004-12-23 RU RU2006126715/13A patent/RU2377300C2/en not_active IP Right Cessation
-
2006
- 2006-06-22 ZA ZA2006/05158A patent/ZA200605158B/en unknown
-
2010
- 2010-02-04 JP JP2010022832A patent/JP2010142243A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ATE462011T1 (en) | 2010-04-15 |
| CN103275944A (en) | 2013-09-04 |
| NZ547085A (en) | 2009-11-27 |
| HK1106953A1 (en) | 2008-03-20 |
| GB0330016D0 (en) | 2004-01-28 |
| RU2377300C2 (en) | 2009-12-27 |
| CN103275944B (en) | 2016-12-07 |
| GB0416023D0 (en) | 2004-08-18 |
| GB0415999D0 (en) | 2004-08-18 |
| CN101676390A (en) | 2010-03-24 |
| RU2006126654A (en) | 2008-01-27 |
| RU2377307C2 (en) | 2009-12-27 |
| DK1704236T3 (en) | 2018-01-08 |
| RU2006126715A (en) | 2008-01-27 |
| JP2010142243A (en) | 2010-07-01 |
| ZA200605158B (en) | 2008-04-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| R082 | Change of representative |
Ref document number: 1862554 Country of ref document: EP Representative=s name: WSL PATENTANWAELTE PARTNERSCHAFTSGESELLSCHAFT, DE |
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| R081 | Change of applicant/patentee |
Ref document number: 1862554 Country of ref document: EP Owner name: DUPONT NUTRITION BIOSCIENCES APS, DK Free format text: FORMER OWNER: DANISCO A/S, KOPENHAGEN/KOEBENHAVN, DK Effective date: 20120821 |
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| R082 | Change of representative |
Ref document number: 1862554 Country of ref document: EP Representative=s name: WSL PATENTANWAELTE PARTNERSCHAFTSGESELLSCHAFT, DE Effective date: 20120821 |