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DE60138344D1 - Verfahren zur Mutagenese - Google Patents

Verfahren zur Mutagenese

Info

Publication number
DE60138344D1
DE60138344D1 DE60138344T DE60138344T DE60138344D1 DE 60138344 D1 DE60138344 D1 DE 60138344D1 DE 60138344 T DE60138344 T DE 60138344T DE 60138344 T DE60138344 T DE 60138344T DE 60138344 D1 DE60138344 D1 DE 60138344D1
Authority
DE
Germany
Prior art keywords
mutagenesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60138344T
Other languages
English (en)
Inventor
Atsushi Miyawaki
Asako Sawano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN
Original Assignee
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN filed Critical RIKEN
Application granted granted Critical
Publication of DE60138344D1 publication Critical patent/DE60138344D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60138344T 2000-08-04 2001-08-03 Verfahren zur Mutagenese Expired - Lifetime DE60138344D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2000237166A JP3859947B2 (ja) 2000-08-04 2000-08-04 突然変異導入方法

Publications (1)

Publication Number Publication Date
DE60138344D1 true DE60138344D1 (de) 2009-05-28

Family

ID=18729074

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60138344T Expired - Lifetime DE60138344D1 (de) 2000-08-04 2001-08-03 Verfahren zur Mutagenese

Country Status (4)

Country Link
US (1) US6673610B2 (de)
EP (1) EP1178109B1 (de)
JP (1) JP3859947B2 (de)
DE (1) DE60138344D1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
DE60232523D1 (de) 2001-07-26 2009-07-16 Stratagene California Multi-stellen-mutagenese
JP4431778B2 (ja) * 2002-07-09 2010-03-17 独立行政法人産業技術総合研究所 組み換えプラスミドの作成方法
WO2006072801A1 (en) * 2005-01-10 2006-07-13 Cambridge Antibody Technology Limited Method of mutagenesis
US20100267147A1 (en) * 2007-04-25 2010-10-21 GM Biosciences, Inc. Site-directed mutagenesis in circular methylated dna
EP2183378A4 (de) 2007-07-31 2010-09-22 Verenium Corp Massgeschneiderte kombinatorische mehrfachstellen-konstruktion
ITTO20070687A1 (it) * 2007-10-01 2009-04-02 Consiglio Nazionale Ricerche Nuovo mutante di gfp sensibile al ph e alla concentrazione di anioni, proteina chimerica comprendente tale mutante e procedimento di determinazione combinata del ph e della concentrazione di anioni
CN101210240B (zh) * 2007-12-25 2011-11-16 天津大学 大引物pcr进行定点突变的方法
WO2013109950A1 (en) * 2012-01-20 2013-07-25 Kennesaw State University Research And Services Foundation Methods to mutate circular deoxyribonucleotides
US9458453B2 (en) 2012-06-12 2016-10-04 The Johns Hopkins University Methods for efficient, expansive, user-defined DNA mutagenesis
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP3322679A4 (de) 2015-07-13 2019-07-10 Pivot Bio, Inc. Verfahren und zusammensetzungen zur verbesserung von pflanzeneigenschaften
AU2016336328A1 (en) 2015-10-05 2018-04-19 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
UY37566A (es) 2017-01-12 2018-07-31 Pivot Bio Inc Métodos y composiciones para mejorar características en plantas
AU2018354221B2 (en) 2017-10-25 2025-04-17 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
BR112020008002A2 (pt) 2017-10-25 2020-11-03 Pivot Bio, Inc. alvos gênicos para fixação de nitrogênio para aprimorar traços de plantas
WO2020006064A2 (en) 2018-06-27 2020-01-02 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
AU2019302747B2 (en) 2018-07-11 2025-11-20 Pivot Bio, Inc. Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes
CN113825393A (zh) 2018-12-21 2021-12-21 皮沃特生物股份有限公司 改善植物性状的方法、组合物和培养基
WO2020132737A2 (en) 2018-12-28 2020-07-02 Braskem S.A. Modulation of carbon flux through the meg and c3 pathways for the improved production of monoethylene glycol and c3 compounds
CA3123940A1 (en) 2019-01-07 2020-07-16 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
US12281299B2 (en) 2019-03-19 2025-04-22 Massachusetts Institute Of Technology Control of nitrogen fixation in rhizobia that associate with cereals
BR112021020165A2 (pt) 2019-04-24 2021-12-21 Pivot Bio Inc Alvos gênicos para fixação direcionada de nitrogênio para melhorar traços de planta
WO2021221690A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
WO2021222643A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Stable liquid formulations for nitrogen-fixing microorganisms
EP4143293A1 (de) 2020-05-01 2023-03-08 Pivot Bio, Inc. Messung der stickstofffixierung und -aufnahme
WO2021222567A2 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
EP4149245A2 (de) 2020-05-13 2023-03-22 Pivot Bio, Inc. Unterdrückung der stickstofffixierung in grampositiven mikroorganismen
AU2022301301A1 (en) 2021-07-02 2023-12-14 Pivot Bio, Inc. Genetically-engineered bacterial strains for improved fixation of nitrogen
CN116083544B (zh) * 2022-03-18 2024-11-08 华中科技大学 一种m6A依赖型限制性内切酶、其突变体及其应用
CN120583887A (zh) 2022-12-23 2025-09-02 皮沃特生物股份有限公司 各种固氮细菌与各种生物产品的组合用于实现协同效应
WO2025155521A1 (en) 2024-01-18 2025-07-24 Pivot Bio, Inc. Host cells for enhanced plant growth
WO2025155518A1 (en) 2024-01-18 2025-07-24 Pivot Bio, Inc. Host cells for enhanced plant growth

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE239089T1 (de) * 1991-12-24 2003-05-15 Harvard College Gezielte punkt-mutagenese von dna
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5789166A (en) 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis

Also Published As

Publication number Publication date
JP3859947B2 (ja) 2006-12-20
US6673610B2 (en) 2004-01-06
JP2002045182A (ja) 2002-02-12
EP1178109A1 (de) 2002-02-06
US20020083488A1 (en) 2002-06-27
EP1178109B1 (de) 2009-04-15

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