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DE60125783D1 - Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay - Google Patents

Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay

Info

Publication number
DE60125783D1
DE60125783D1 DE60125783T DE60125783T DE60125783D1 DE 60125783 D1 DE60125783 D1 DE 60125783D1 DE 60125783 T DE60125783 T DE 60125783T DE 60125783 T DE60125783 T DE 60125783T DE 60125783 D1 DE60125783 D1 DE 60125783D1
Authority
DE
Germany
Prior art keywords
modified
acetylase
immunoassay
peptide
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60125783T
Other languages
English (en)
Other versions
DE60125783T2 (de
Inventor
Joachim Kraemer
Christine Peiker
Karsten Henco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evotec OAI AG
Original Assignee
Evotec OAI AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00117457A external-priority patent/EP1182263A1/de
Priority claimed from EP00122707A external-priority patent/EP1199370B1/de
Priority claimed from EP00128176A external-priority patent/EP1217078A1/de
Application filed by Evotec OAI AG filed Critical Evotec OAI AG
Publication of DE60125783D1 publication Critical patent/DE60125783D1/de
Application granted granted Critical
Publication of DE60125783T2 publication Critical patent/DE60125783T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/081—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0812—Tripeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/1013—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60125783T 2000-08-11 2001-08-13 Verfahren zum nachweis von acetylase oder deacetylase-aktivität in einem immunoassay Expired - Lifetime DE60125783T2 (de)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
EP00117457 2000-08-11
EP00117457A EP1182263A1 (de) 2000-08-11 2000-08-11 Verfahren zum Nachweis einer Threonin- oder Serinkinasenaktivität
EP00118431 2000-08-24
EP00118431 2000-08-24
EP00122707 2000-10-18
EP00122707A EP1199370B1 (de) 2000-10-18 2000-10-18 Verfahren zur Auffindung von Dephosphorylierung phosphorylierte Threonin oder Serin durch Phosphataseaktivität
EP00128176A EP1217078A1 (de) 2000-12-21 2000-12-21 Phosphatase-Immunoassay mit Antikörper gegen bisphosphorylierte Substrate
EP00128176 2000-12-21
PCT/EP2001/009354 WO2002014543A2 (en) 2000-08-11 2001-08-13 Process for detecting enzyme activity in an immunoassay

Publications (2)

Publication Number Publication Date
DE60125783D1 true DE60125783D1 (de) 2007-02-15
DE60125783T2 DE60125783T2 (de) 2007-10-18

Family

ID=27439967

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60125783T Expired - Lifetime DE60125783T2 (de) 2000-08-11 2001-08-13 Verfahren zum nachweis von acetylase oder deacetylase-aktivität in einem immunoassay

Country Status (5)

Country Link
EP (1) EP1313876B8 (de)
AT (1) ATE350485T1 (de)
AU (1) AU2001293768A1 (de)
DE (1) DE60125783T2 (de)
WO (1) WO2002014543A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005030947A2 (en) 2003-09-30 2005-04-07 Evotec Neurosciences Gmbh Diagnostic and therapeutic use of a sulfotransferase for neurodegenerative diseases
ES2380679T3 (es) * 2005-03-03 2012-05-17 Sirtris Pharmaceuticals, Inc. Ensayos de polarización de fluorescencia para la actividad acetiltransferasa/desacetilasa
EP1891234B1 (de) 2005-06-01 2014-12-10 Evotec International GmbH Verwendung von slc39a12-proteinen als zielmoleküle in der diagnose von und beim screening von medikamenten zur behandlung von alzheimer-krankheit
DE602006019582D1 (de) 2005-06-16 2011-02-24 Evotec Neurosciences Gmbh Adarb2 proteine als diagnostische und therapeutische targets für neurodegenerative erkrankungen
CN101313218A (zh) * 2005-11-25 2008-11-26 皇家飞利浦电子股份有限公司 用于酶活性确定的磁性生物传感器
ES2389111T3 (es) 2005-12-02 2012-10-23 Sirtris Pharmaceuticals, Inc. Ensayos de espectrometría de masas para la actividad acetiltransferasa/desacetilasa
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2192801T3 (es) * 1997-12-05 2003-10-16 Upjohn Co Ensayos de criba de alta productividad de proteina quinasas y fosfatasas, basados en fluorescencia.

Also Published As

Publication number Publication date
ATE350485T1 (de) 2007-01-15
EP1313876A2 (de) 2003-05-28
AU2001293768A1 (en) 2002-02-25
WO2002014543A3 (en) 2003-03-20
EP1313876B8 (de) 2007-02-28
WO2002014543A2 (en) 2002-02-21
EP1313876B1 (de) 2007-01-03
DE60125783T2 (de) 2007-10-18

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: EVOTEC AG, 22525 HAMBURG, DE

8364 No opposition during term of opposition