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DE60117180D1 - Verfahren zur messung des aktivierungszustands von signalwegen in zellen - Google Patents

Verfahren zur messung des aktivierungszustands von signalwegen in zellen

Info

Publication number
DE60117180D1
DE60117180D1 DE60117180T DE60117180T DE60117180D1 DE 60117180 D1 DE60117180 D1 DE 60117180D1 DE 60117180 T DE60117180 T DE 60117180T DE 60117180 T DE60117180 T DE 60117180T DE 60117180 D1 DE60117180 D1 DE 60117180D1
Authority
DE
Germany
Prior art keywords
reporter
amount
cell
enzyme
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60117180T
Other languages
English (en)
Other versions
DE60117180T2 (de
Inventor
L Allbritton
Christopher Sims
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
University of California Berkeley
Original Assignee
University of California
University of California Berkeley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California, University of California Berkeley filed Critical University of California
Publication of DE60117180D1 publication Critical patent/DE60117180D1/de
Application granted granted Critical
Publication of DE60117180T2 publication Critical patent/DE60117180T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60117180T 2000-11-22 2001-11-21 Verfahren zur messung des aktivierungszustands von signalwegen in zellen Expired - Fee Related DE60117180T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25286100P 2000-11-22 2000-11-22
US252861P 2000-11-22
PCT/US2001/043966 WO2002042732A2 (en) 2000-11-22 2001-11-21 A method to measure the activation state of signaling pathways in cells

Publications (2)

Publication Number Publication Date
DE60117180D1 true DE60117180D1 (de) 2006-04-20
DE60117180T2 DE60117180T2 (de) 2006-08-10

Family

ID=22957857

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60117180T Expired - Fee Related DE60117180T2 (de) 2000-11-22 2001-11-21 Verfahren zur messung des aktivierungszustands von signalwegen in zellen

Country Status (6)

Country Link
EP (1) EP1336101B1 (de)
JP (1) JP2004514885A (de)
AT (1) ATE317546T1 (de)
AU (1) AU2002217839A1 (de)
DE (1) DE60117180T2 (de)
WO (1) WO2002042732A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335201B1 (en) 1998-03-06 2002-01-01 The Regents Of The University Of California Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility
US6740497B2 (en) * 1998-03-06 2004-05-25 The Regents Of The University Of California Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
DE102005059536B4 (de) 2005-12-13 2008-08-28 Siemens Ag Verfahren und Biochip zur Untersuchung einer biologischen Probe
JP2011512125A (ja) * 2008-02-01 2011-04-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア マイクロ流体画像サイトメトリ
CN101231262B (zh) * 2008-02-22 2011-09-14 东南大学 用于单细胞组学的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258289A (en) * 1990-09-05 1993-11-02 Davis Claude G Method for the selecting of genes encoding catalytic antibodies
US5156576B1 (en) * 1991-05-22 1994-08-30 Sauer Inc Compact integrated transaxle
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6335201B1 (en) * 1998-03-06 2002-01-01 The Regents Of The University Of California Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility
US6156576A (en) * 1998-03-06 2000-12-05 The Regents Of The University Of California Fast controllable laser lysis of cells for analysis
AU6189599A (en) * 1998-10-15 2000-05-08 Bioimage A/S An improved method for extracting quantitative information relating to an influence on a cellular response
AU3605600A (en) * 1999-02-26 2000-09-14 Cellomics, Inc. A system for cell-based screening
AU2579401A (en) * 1999-12-17 2001-06-25 Johns Hopkins University, The Brain protein markers

Also Published As

Publication number Publication date
AU2002217839A1 (en) 2002-06-03
EP1336101A4 (de) 2004-08-18
WO2002042732A3 (en) 2002-08-01
JP2004514885A (ja) 2004-05-20
WO2002042732A2 (en) 2002-05-30
EP1336101A2 (de) 2003-08-20
EP1336101B1 (de) 2006-02-08
DE60117180T2 (de) 2006-08-10
ATE317546T1 (de) 2006-02-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee