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DE60042396D1 - Hohe expression eines heterologen proteins mit seltenen codons - Google Patents

Hohe expression eines heterologen proteins mit seltenen codons

Info

Publication number
DE60042396D1
DE60042396D1 DE60042396T DE60042396T DE60042396D1 DE 60042396 D1 DE60042396 D1 DE 60042396D1 DE 60042396 T DE60042396 T DE 60042396T DE 60042396 T DE60042396 T DE 60042396T DE 60042396 D1 DE60042396 D1 DE 60042396D1
Authority
DE
Germany
Prior art keywords
host cells
expression
high expression
rare codons
rare
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60042396T
Other languages
English (en)
Inventor
Carsten-Peter Carstens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CARSTENS, CARSTEN-PETER, LA JOLLA, CALIF., US
Agilent Technologies Inc
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of DE60042396D1 publication Critical patent/DE60042396D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Lubricants (AREA)
DE60042396T 1999-01-27 2000-01-27 Hohe expression eines heterologen proteins mit seltenen codons Expired - Lifetime DE60042396D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11735599P 1999-01-27 1999-01-27
PCT/US2000/002002 WO2000044926A1 (en) 1999-01-27 2000-01-27 High level expression of a heterologous protein having rare codons

Publications (1)

Publication Number Publication Date
DE60042396D1 true DE60042396D1 (de) 2009-07-30

Family

ID=22372458

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60042396T Expired - Lifetime DE60042396D1 (de) 1999-01-27 2000-01-27 Hohe expression eines heterologen proteins mit seltenen codons

Country Status (6)

Country Link
US (1) US20040214281A1 (de)
EP (1) EP1147211B1 (de)
AT (1) ATE434051T1 (de)
CA (1) CA2360352A1 (de)
DE (1) DE60042396D1 (de)
WO (1) WO2000044926A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2358385C (en) 1998-12-31 2013-08-06 Chiron Corporation Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof
WO2000039302A2 (en) 1998-12-31 2000-07-06 Chiron Corporation Improved expression of hiv polypeptides and production of virus-like particles
US20110097709A1 (en) * 2000-03-13 2011-04-28 Kidd Geoffrey L Method for modifying a nucleic acid
DE10037111A1 (de) * 2000-07-27 2002-02-07 Boehringer Ingelheim Int Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle
US6821755B2 (en) 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
US7211659B2 (en) 2001-07-05 2007-05-01 Chiron Corporation Polynucleotides encoding antigenic HIV type C polypeptides, polypeptides and uses thereof
US20030170614A1 (en) 2001-08-31 2003-09-11 Megede Jan Zur Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof
JPWO2003083101A1 (ja) * 2002-03-29 2005-08-04 三菱ウェルファーマ株式会社 Ｄａｐキナーゼの結晶、それを用いたｄａｐキナーゼをターゲットとする薬物候補分子のスクリーニング方法及びそれにより得られる医薬
WO2004024915A1 (en) 2002-09-13 2004-03-25 The University Of Queensland Gene expression system based on codon translation efficiency
WO2006055836A2 (en) * 2004-11-16 2006-05-26 President And Fellows Of Harvard College In vivo alteration of cellular dna
JP5232138B2 (ja) 2006-04-22 2013-07-10 スカラブ ゲノミクス， エルエルシー ｔＲＮＡ遺伝子をしようする、組み換えタンパク質産生のための方法および組成物
US8361746B2 (en) * 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
KR101488110B1 (ko) 2013-11-29 2015-01-29 성균관대학교산학협력단 나노아케움 이퀴탄스 유래의 Neq HS DNA 중합효소의 돌연변이체 제조 및 이를 이용한 hot-start PCR 응용
US10724040B2 (en) * 2015-07-15 2020-07-28 The Penn State Research Foundation mRNA sequences to control co-translational folding of proteins
CN105274042B (zh) * 2015-11-24 2019-05-21 山东省医学科学院基础医学研究所 一株重组大肠杆菌及其在发酵生产2[4Fe4S]铁氧还蛋白中的应用
CN105349480B (zh) * 2015-11-24 2019-03-05 山东省医学科学院基础医学研究所 一株高效表达[2Fe2S]铁氧还蛋白的重组大肠杆菌及其应用
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU618640B2 (en) * 1988-11-11 1992-01-02 Boehringer Mannheim Gmbh Process for the expression of a recombinant gene
DE4417598A1 (de) * 1994-05-19 1995-12-14 Max Planck Gesellschaft Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen
US6013505A (en) * 1996-10-08 2000-01-11 Smithkline Beecham Corporation Topoisomerase I

Also Published As

Publication number Publication date
EP1147211B1 (de) 2009-06-17
EP1147211A1 (de) 2001-10-24
WO2000044926A1 (en) 2000-08-03
US20040214281A1 (en) 2004-10-28
CA2360352A1 (en) 2000-08-03
ATE434051T1 (de) 2009-07-15
EP1147211A4 (de) 2002-08-21

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: AGILENT TECHNOLOGIES, INC. (N.D.GES.D. STAATES, US

Owner name: CARSTENS, CARSTEN-PETER, LA JOLLA, CALIF., US