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DE60042209D1 - Enzymatische synthese von deoxyribonucleotiden - Google Patents

Enzymatische synthese von deoxyribonucleotiden

Info

Publication number
DE60042209D1
DE60042209D1 DE60042209T DE60042209T DE60042209D1 DE 60042209 D1 DE60042209 D1 DE 60042209D1 DE 60042209 T DE60042209 T DE 60042209T DE 60042209 T DE60042209 T DE 60042209T DE 60042209 D1 DE60042209 D1 DE 60042209D1
Authority
DE
Germany
Prior art keywords
deoxyribonucleotides
enzymatic synthesis
synthesis
enzymatic
deoxyribonucleosides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60042209T
Other languages
English (en)
Inventor
Wilhelm Tischer
Hans-Georg Ihlenfeldt
Octavian Barzu
Hiroshi Sakamoto
Elisabeth Pistotnik
Philippe Marliere
Sylvie Pochet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur
Original Assignee
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8238824&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60042209(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Institut Pasteur filed Critical Institut Pasteur
Application granted granted Critical
Publication of DE60042209D1 publication Critical patent/DE60042209D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60042209T 1999-08-20 2000-08-18 Enzymatische synthese von deoxyribonucleotiden Expired - Lifetime DE60042209D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99116425 1999-08-20
PCT/EP2000/008088 WO2001014566A2 (en) 1999-08-20 2000-08-18 Enzymatic synthesis of deoxyribonucleosides

Publications (1)

Publication Number Publication Date
DE60042209D1 true DE60042209D1 (de) 2009-06-25

Family

ID=8238824

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60042209T Expired - Lifetime DE60042209D1 (de) 1999-08-20 2000-08-18 Enzymatische synthese von deoxyribonucleotiden

Country Status (10)

Country Link
US (2) US7229797B1 (de)
EP (1) EP1206551B2 (de)
JP (1) JP2003507071A (de)
KR (1) KR20020048930A (de)
AT (1) ATE431416T1 (de)
AU (1) AU6571700A (de)
CA (1) CA2382462A1 (de)
DE (1) DE60042209D1 (de)
HU (1) HUP0203049A3 (de)
WO (1) WO2001014566A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2412377A1 (en) 2000-06-06 2001-12-13 Bristol-Myers Squibb Company B7-related nucleic acids and polypeptides and their uses for immunomodulation
US20030031675A1 (en) 2000-06-06 2003-02-13 Mikesell Glen E. B7-related nucleic acids and polypeptides useful for immunomodulation
US6858721B2 (en) 2001-05-01 2005-02-22 Mitsui Chemicals, Inc. Method for producing cytosine nucleoside compounds
US7381555B2 (en) 2001-09-14 2008-06-03 Institut Pasteur Lactobacillus N-deoxyribosyl transferases, corresponding nucleotide sequences and their uses
FR2829774B1 (fr) * 2001-09-14 2004-09-03 Pasteur Institut N-desoxyribosyltransferases de lactobacilles, sequences nucleotidiques correspondantes et leurs applications
US20040214314A1 (en) 2001-11-02 2004-10-28 Friedrich Srienc High throughput bioreactor
EP1491549A1 (de) * 2003-06-24 2004-12-29 Evologic S.A. Herstellung von 2'-Deoxynukleoside und 2'-Deoxynukleosidvorläufern von 2-Dehydro-3-Deoxy-D-Gluconat
KR100817462B1 (ko) * 2003-06-24 2008-03-31 에볼로직 소시에떼 아노님 2-데하이드로-3-데옥시-ｄ-글루코네이트로부터의2'-데옥시뉴클레오시드 및 2'-데옥시뉴클레오시드전구체들의 제조
GB2403950A (en) * 2003-07-18 2005-01-19 Mitsui Chemicals Inc Pyrimidine nucleosides from reaction of sugar phosphate with pyrimidine base derivative & enzyme having cytosine nucleoside phosphorylase activity
JP4437786B2 (ja) * 2003-11-11 2010-03-24 三井化学株式会社 ペントース−５−リン酸エステルの製造方法。
FR2853906B1 (fr) * 2004-06-07 2006-04-14 Pasteur Institut N-desoxyribosyltransferases de lactobacilles, sequences nucleotidiques correspondantes et leurs applications
PT2660336T (pt) * 2008-06-11 2017-09-13 Orion Pharma (Uk) Ltd Amplificação isotérmica de ácidos nucleicos
EP2917341B1 (de) * 2012-11-08 2017-09-27 Centre National de la Recherche Scientifique (CNRS) Neue mutante phosphodeoxyribosyltransferase-enzyme und verwendungen davon
EP2992107B1 (de) * 2013-04-29 2019-12-11 Plasmia Biotech, S.L. Biokatalytische herstellung von nukleosid-analogen als pharmazeutische wirkstoffe
JP2015160832A (ja) * 2014-02-27 2015-09-07 大陽日酸株式会社 ヌクレオシド化合物の製造方法
SG11202012109UA (en) * 2018-07-09 2021-01-28 Codexis Inc Engineered phosphopentomutase variant enzymes
EP3820473A4 (de) 2018-07-09 2022-05-11 Merck Sharp & Dohme Corp. Enzymatische synthese von 4'-ethinylnukleosidanaloga
CA3103718A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
CN114072165A (zh) 2019-07-02 2022-02-18 科德克希思公司 工程化蔗糖磷酸化酶变体酶
CN110592084B (zh) * 2019-08-28 2023-07-28 内蒙古伊品生物科技有限公司 一种rhtA基因启动子改造的重组菌株及其构建方法与应用
KR102768742B1 (ko) * 2019-08-28 2025-02-14 이너 몽골리아, 에펜 바이오테크 컴퍼니 리미티드 대장균 기반 재조합 균주 및 이의 구축 방법과 응용

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3168585D1 (en) 1980-04-23 1985-03-14 Wellcome Found Deazapurine nucleosides, formulations and preparation thereof
JPS5863393A (ja) 1981-10-13 1983-04-15 Ajinomoto Co Inc リボノフラノシル又はデオキシリボフラノシルプリン誘導体の製造法
EP0090417B1 (de) 1982-04-01 1989-03-01 Yamasa Shoyu Kabushiki Kaisha Verfahren zur Herstellung von 3'-Deoxyguanosin
ES2089010T3 (es) 1989-02-28 1996-10-01 Yamasa Shoyu Kk Procedimiento para la obtencion de nucleosidos mediante la utilizacion de fosforilasas obtenibles a partir de bacillus stearothermophilus.
DK0593757T3 (da) * 1989-07-31 1997-07-07 Inst Of Protein Research Russi Fremgangsmåde til opnåelse af polypeptider i et cellefrit system
US5728554A (en) * 1995-04-11 1998-03-17 Cytel Corporation Enzymatic synthesis of glycosidic linkages

Also Published As

Publication number Publication date
EP1206551A2 (de) 2002-05-22
EP1206551B2 (de) 2011-10-26
WO2001014566A3 (en) 2001-09-20
JP2003507071A (ja) 2003-02-25
HUP0203049A2 (hu) 2002-12-28
EP1206551B1 (de) 2009-05-13
AU6571700A (en) 2001-03-19
US7229797B1 (en) 2007-06-12
HUP0203049A3 (en) 2007-03-28
CA2382462A1 (en) 2001-03-01
WO2001014566A2 (en) 2001-03-01
ATE431416T1 (de) 2009-05-15
US20080280329A1 (en) 2008-11-13
KR20020048930A (ko) 2002-06-24

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