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DE60041442D1 - Isolierung von nukleinsäuren - Google Patents

Isolierung von nukleinsäuren

Info

Publication number
DE60041442D1
DE60041442D1 DE60041442T DE60041442T DE60041442D1 DE 60041442 D1 DE60041442 D1 DE 60041442D1 DE 60041442 T DE60041442 T DE 60041442T DE 60041442 T DE60041442 T DE 60041442T DE 60041442 D1 DE60041442 D1 DE 60041442D1
Authority
DE
Germany
Prior art keywords
substituent
sample
isolation
nucleic acids
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60041442T
Other languages
English (en)
Inventor
Andrew Simon Goldsborough
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cyclops Genome Sciences Ltd
Original Assignee
Cyclops Genome Sciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9910154.5A external-priority patent/GB9910154D0/en
Priority claimed from GBGB9910157.8A external-priority patent/GB9910157D0/en
Priority claimed from GBGB9910158.6A external-priority patent/GB9910158D0/en
Priority claimed from GBGB9910156.0A external-priority patent/GB9910156D0/en
Application filed by Cyclops Genome Sciences Ltd filed Critical Cyclops Genome Sciences Ltd
Application granted granted Critical
Publication of DE60041442D1 publication Critical patent/DE60041442D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Turbine Rotor Nozzle Sealing (AREA)
  • Window Of Vehicle (AREA)
DE60041442T 1999-04-30 2000-05-02 Isolierung von nukleinsäuren Expired - Fee Related DE60041442D1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9910154.5A GB9910154D0 (en) 1999-04-30 1999-04-30 Polynucleotides
GBGB9910157.8A GB9910157D0 (en) 1999-04-30 1999-04-30 Polynucleotides
GBGB9910158.6A GB9910158D0 (en) 1999-04-30 1999-04-30 Isolation of nucleic acid
GBGB9910156.0A GB9910156D0 (en) 1999-04-30 1999-04-30 Polynucleotides
PCT/GB2000/001684 WO2000075302A2 (en) 1999-04-30 2000-05-02 Isolation of nucleic acid

Publications (1)

Publication Number Publication Date
DE60041442D1 true DE60041442D1 (de) 2009-03-12

Family

ID=27451902

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60032235T Expired - Lifetime DE60032235T2 (de) 1999-04-30 2000-05-02 Ribonukleinsäurederivate
DE60041442T Expired - Fee Related DE60041442D1 (de) 1999-04-30 2000-05-02 Isolierung von nukleinsäuren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60032235T Expired - Lifetime DE60032235T2 (de) 1999-04-30 2000-05-02 Ribonukleinsäurederivate

Country Status (6)

Country Link
US (4) US6794140B1 (de)
EP (2) EP1177281B1 (de)
AT (2) ATE347616T1 (de)
AU (4) AU4589000A (de)
DE (2) DE60032235T2 (de)
WO (4) WO2000066605A2 (de)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP1177281B1 (de) 1999-04-30 2009-01-21 Cyclops Genome Sciences Limited Isolierung von nukleinsäuren
US6677120B2 (en) 2001-03-30 2004-01-13 Isis Pharmaceuticals, Inc. Building blocks for the solution phase synthesis of oligonucleotides
US20060009409A1 (en) * 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
EP2221377B2 (de) 2002-02-01 2017-05-17 Life Technologies Corporation Doppelsträngige Oligonukleotide
US20040005568A1 (en) * 2002-07-03 2004-01-08 Stojanovic Milan N. Movement of multi-enzymatic nanoassemblies on recognition landscapes
US20040072226A1 (en) * 2002-07-03 2004-04-15 Stojanovic Milan N. Movement of multi-enzymatic nanoassemblies on recognition landscapes
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
DE10315581B4 (de) * 2003-04-05 2007-06-28 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto Verfahren zur Qualitätsbestimmung von RNA-Proben
EP1649016A1 (de) * 2003-07-23 2006-04-26 Cyclops Genome Sciences Limited Reinigungskügelchen
GB0317199D0 (en) * 2003-07-23 2003-08-27 Cyclops Genome Sciences Ltd Clean-up beads
DE10344819B4 (de) * 2003-09-26 2017-06-29 Sartorius Stedim Biotech Gmbh Adsorptionsmembranen, Verfahren zur Herstellung derselben und Vorrichtungen, welche die Adsorptionsmembranen umfassen
KR20130143677A (ko) 2004-11-05 2013-12-31 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법
US20090075342A1 (en) * 2005-04-26 2009-03-19 Sharon Cload Metabolic profile directed aptamer medicinal chemistry
US8252756B2 (en) 2005-06-14 2012-08-28 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US8318424B2 (en) * 2006-06-05 2012-11-27 The University Of North Carolina At Chapel Hill High-throughput RNA structure analysis
JP5512285B2 (ja) * 2007-02-09 2014-06-04 ノースウェスタン ユニバーシティ 細胞内標的を検出するための粒子
EP2142671A2 (de) * 2007-03-30 2010-01-13 Qiagen GmbH Modifizierte polynukleotide mit riboseringen
WO2008151049A2 (en) 2007-05-30 2008-12-11 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
US9551033B2 (en) 2007-06-08 2017-01-24 Genentech, Inc. Gene expression markers of tumor resistance to HER2 inhibitor treatment
CA2729613C (en) 2008-03-03 2017-02-07 Tosk, Incorporated Methotrexate adjuvants to reduce toxicity and methods for using the same
EP2235177B1 (de) * 2008-06-13 2012-07-18 RiboxX GmbH Verfahren zur enzymatischen synthese chemisch modifizierter rna
EP2365803B1 (de) * 2008-11-24 2017-11-01 Northwestern University Polyvalente rna-nanoteilchen-zusammensetzungen
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
MX2011008563A (es) 2009-02-13 2012-06-19 Chem Inc X Metodos de crear y analizar/seleccionar bibliotecas codificadas por adn.
EP2272976A1 (de) * 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Differenzierung von Polynucleotid-Strängen
US20120185176A1 (en) 2009-09-30 2012-07-19 Natera, Inc. Methods for Non-Invasive Prenatal Ploidy Calling
JP5866119B2 (ja) 2009-10-30 2016-02-17 ノースウェスタン ユニバーシティ 鋳型ナノ複合体
EP2333102A1 (de) * 2009-12-14 2011-06-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Identifizierung von RNA-Liganden
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2572003A4 (de) 2010-05-18 2016-01-13 Natera Inc Verfahren zur nichtinvasiven pränatalen ploidiezuordnung
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
EP2388324A1 (de) 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Kontrollmaterial für Nukleinsäureteste
FR2968302B1 (fr) 2010-12-06 2012-11-30 Biomerieux Sa Procedes de fonctionalisation et reactifs utilises dans de tels procedes utilisant un anhydride isatoique ou un de ses derives, molecules biologiques ainsi traitees et kits
JP6328934B2 (ja) 2010-12-22 2018-05-23 ナテラ, インコーポレイテッド 非侵襲性出生前親子鑑定法
BR112013020220B1 (pt) 2011-02-09 2020-03-17 Natera, Inc. Método para determinar o estado de ploidia de um cromossomo em um feto em gestação
CA2848023C (en) * 2011-09-07 2022-03-15 X-Chem, Inc. Methods for tagging dna-encoded libraries
CN104023749B (zh) 2011-09-14 2019-12-17 西北大学 能穿过血脑屏障的纳米缀合物
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
ES2855137T3 (es) 2012-07-13 2021-09-23 X Chem Inc Bibliotecas codificadas por ADN que tienen vínculos de oligonucleótidos codificadores que no se pueden leer por polimerasas
FR2994184B1 (fr) 2012-08-02 2017-12-08 Biomerieux Sa Procedes de fonctionalisation et reactifs utilises dans de tels procedes utilisant un anhydride aza-isatoique ou un de ses derives, molecules biologiques ainsi traitees et kits
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US9970002B2 (en) * 2012-12-12 2018-05-15 Massachusetts Institute Of Technology Compositions and methods for functional nucleic acid delivery
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
EP3055413B1 (de) 2013-10-07 2020-01-08 The University of North Carolina at Chapel Hill Nachweis chemischer modifikationen in nukleinsäuren
CA2945962C (en) 2014-04-21 2023-08-29 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
KR20250005450A (ko) 2014-04-25 2025-01-09 샤이어 휴먼 지네틱 테라피즈 인크. 메신저 rna 의 정제 방법
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
CN107405540A (zh) * 2014-10-24 2017-11-28 雅培分子公司 小核酸的富集
WO2016081911A2 (en) 2014-11-21 2016-05-26 Northwestern University The sequence-specific cellular uptake of spherical nucleic acid nanoparticle conjugates
DK3294885T3 (da) 2015-05-08 2020-08-10 Curevac Real Estate Gmbh Fremgangsmåde til at fremstille rna
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
WO2016193206A1 (en) 2015-05-29 2016-12-08 Curevac Ag A method for producing and purifying rna, comprising at least one step of tangential flow filtration
SG10201911134QA (en) 2015-06-05 2020-01-30 Grace W R & Co Adsorbent bioprocessing clarification agents and methods of making and using the same
GB2578075B (en) 2015-09-09 2020-08-26 Drawbridge Health Inc Systems, methods, and devices for sample collection, stabilization and preservation
US10214739B2 (en) * 2015-10-30 2019-02-26 Axagarius Gmbh & Co. Kg RNA binding solution
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
WO2018085752A2 (en) * 2016-11-07 2018-05-11 nanoSUR LLC Post-transcriptionally chemically modified double strand rnas
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CN210383905U (zh) 2017-01-10 2020-04-24 集联健康有限公司 一种用于从受试者收集流体样品的装置以及运输套筒
CA3049139A1 (en) * 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
EA202090752A1 (ru) * 2017-06-16 2020-06-15 Борис Славинович ФАРБЕР Комбинаторные производные олигонуклеотидов рнк
WO2019109046A1 (en) 2017-11-30 2019-06-06 Arrakis Therapeutics, Inc. Nucleic acid-binding photoprobes and uses thereof
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
FR3078977B1 (fr) * 2018-03-16 2023-03-17 Biomerieux Sa Procede d'extraction d'acides nucleiques
EP3781714A1 (de) 2018-04-14 2021-02-24 Natera, Inc. Verfahren zur krebserkennung und -überwachung durch personalisierte detektion zirkulierender tumor-dna
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
US11446303B2 (en) 2019-06-21 2022-09-20 Tosk, Inc. Uridine phosphorylase (UPase) inhibitors for treatment of liver conditions
JP2024532413A (ja) * 2021-08-31 2024-09-05 ナノスール エルエルシー 高分子量修飾dsRNA組成物
US20250027084A1 (en) * 2021-11-30 2025-01-23 Northwestern University Compositions for inhibiting dipeptide repeat protein-ribosomal rna interaction and uses thereof
KR20250141812A (ko) * 2023-02-06 2025-09-29 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 Rna에 대한 선천성 면역 반응을 감소시키기 위한 방법 및 변형
US20250270299A1 (en) * 2024-01-31 2025-08-28 Helix Nanotechnologies Inc Methods of using compositions comprising sbi domains

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588718A (en) * 1984-03-28 1986-05-13 The Upjohn Company Carboxy containing ester prodrugs of corticosteroids
US5681726A (en) * 1988-09-19 1997-10-28 Stratagene Method of double stranded DNA synthesis
US5872232A (en) * 1990-01-11 1999-02-16 Isis Pharmaceuticals Inc. 2'-O-modified oligonucleotides
US5859221A (en) * 1990-01-11 1999-01-12 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US5707796A (en) * 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
CA2089376C (en) 1990-08-13 2010-05-04 Phillip Dan Cook Sugar modified oligonucleotides that detect and modulate gene expression
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
US6235887B1 (en) * 1991-11-26 2001-05-22 Isis Pharmaceuticals, Inc. Enhanced triple-helix and double-helix formation directed by oligonucleotides containing modified pyrimidines
WO1994019012A2 (en) 1993-02-24 1994-09-01 Wang Jui H Compositions and methods of application of reactive antiviral polymers
ATE174600T1 (de) * 1993-10-27 1999-01-15 Ribozyme Pharm Inc 2'-amido-und 2'-peptido-modifizierte oligonukleotide
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
CA2261892A1 (en) * 1996-07-24 1998-01-29 Michelle M. Hanna Base-protected nucleotide analogs with protected thiol groups
US5849902A (en) 1996-09-26 1998-12-15 Oligos Etc. Inc. Three component chimeric antisense oligonucleotides
WO1999014346A2 (en) 1997-09-19 1999-03-25 Sequitur, Inc. SENSE mRNA THERAPY
DE19801153A1 (de) 1998-01-14 1999-07-15 Jenne Andreas Verfahren zur Selektion von Ribozymen, die Ribonucleinsäuren in trans an 2'-OH-Gruppen kovalent modifizieren können und durch dieses Verfahren erhältliche Ribozyme
WO1999055857A2 (en) 1998-04-29 1999-11-04 Ribozyme Pharmaceuticals, Inc. Nucleoside triphosphates and their incorporation into ribozymes
AU5728999A (en) 1998-07-28 2000-02-21 Micromet Ag Heterominibodies
EP1177281B1 (de) * 1999-04-30 2009-01-21 Cyclops Genome Sciences Limited Isolierung von nukleinsäuren
US6720144B1 (en) * 2001-06-21 2004-04-13 Quest Diagnostics Detection of clonal T-cell receptor-γ gene rearrangement by PCR/temporal temperature gradient gel electrophoresis (TTGE)
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
EP1649016A1 (de) 2003-07-23 2006-04-26 Cyclops Genome Sciences Limited Reinigungskügelchen

Also Published As

Publication number Publication date
US20030039985A1 (en) 2003-02-27
US20050074753A1 (en) 2005-04-07
US7244568B2 (en) 2007-07-17
AU4589000A (en) 2000-11-17
AU4767300A (en) 2001-12-17
WO2000066605A2 (en) 2000-11-09
AU4588000A (en) 2000-12-28
US20050272679A1 (en) 2005-12-08
EP1196631B1 (de) 2006-12-06
EP1177281B1 (de) 2009-01-21
AU4767400A (en) 2000-12-28
DE60032235T2 (de) 2007-11-15
DE60032235D1 (de) 2007-01-18
WO2001094626A1 (en) 2001-12-13
US6794140B1 (en) 2004-09-21
WO2000075306A3 (en) 2001-05-17
WO2000066605A3 (en) 2001-04-26
ATE347616T1 (de) 2006-12-15
WO2000075302A2 (en) 2000-12-14
EP1196631A1 (de) 2002-04-17
WO2000075302A3 (en) 2001-04-26
WO2000075306A2 (en) 2000-12-14
US6867290B2 (en) 2005-03-15
ATE421583T1 (de) 2009-02-15
EP1177281A2 (de) 2002-02-06

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