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DE60028777D1 - PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A RECOMBINANT MICRO ORGANISM IN THE ABSENCE OF COENZYME B12 - Google Patents

PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A RECOMBINANT MICRO ORGANISM IN THE ABSENCE OF COENZYME B12

Info

Publication number
DE60028777D1
DE60028777D1 DE60028777T DE60028777T DE60028777D1 DE 60028777 D1 DE60028777 D1 DE 60028777D1 DE 60028777 T DE60028777 T DE 60028777T DE 60028777 T DE60028777 T DE 60028777T DE 60028777 D1 DE60028777 D1 DE 60028777D1
Authority
DE
Germany
Prior art keywords
coenzyme
propanediol
absence
preparation
concerns
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60028777T
Other languages
German (de)
Other versions
DE60028777T2 (en
Inventor
Patricia Sarcabal
Christian Croux
Philippe Soucaille
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INST NAT SCIENCES APPLIQ
Centre National de la Recherche Scientifique CNRS
Institut National des Sciences Appliquees de Toulouse INSA
Institut National de la Recherche Agronomique INRA
Original Assignee
INST NAT SCIENCES APPLIQ
Centre National de la Recherche Scientifique CNRS
Institut National des Sciences Appliquees de Toulouse INSA
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INST NAT SCIENCES APPLIQ, Centre National de la Recherche Scientifique CNRS, Institut National des Sciences Appliquees de Toulouse INSA, Institut National de la Recherche Agronomique INRA filed Critical INST NAT SCIENCES APPLIQ
Publication of DE60028777D1 publication Critical patent/DE60028777D1/en
Application granted granted Critical
Publication of DE60028777T2 publication Critical patent/DE60028777T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.
DE60028777T 1999-07-09 2000-07-07 PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A RECOMBINANT MICROORGANISM IN THE ABSENCE OF COENZYME B12 Expired - Lifetime DE60028777T2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR9908939A FR2796081B1 (en) 1999-07-09 1999-07-09 PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A MICROORGANISM RECOMBINANT IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS
FR9908939 1999-07-09
PCT/FR2000/001981 WO2001004324A1 (en) 1999-07-09 2000-07-07 Method for preparing 1,3-propanediol by a recombinant micro-organism in the absence of coenzyme b12 or one of its precursors
US10/043,639 US7267972B2 (en) 1999-07-09 2002-01-09 Method for preparing 1,3-propanediol by a recombinant micro-organism in the absence of coenzyme b12 or one of its precursors

Publications (2)

Publication Number Publication Date
DE60028777D1 true DE60028777D1 (en) 2006-07-27
DE60028777T2 DE60028777T2 (en) 2007-05-31

Family

ID=29720861

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60028777T Expired - Lifetime DE60028777T2 (en) 1999-07-09 2000-07-07 PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A RECOMBINANT MICROORGANISM IN THE ABSENCE OF COENZYME B12

Country Status (11)

Country Link
US (3) US7267972B2 (en)
EP (1) EP1200597B1 (en)
AT (1) ATE330014T1 (en)
AU (1) AU6296400A (en)
CY (1) CY1105334T1 (en)
DE (1) DE60028777T2 (en)
DK (1) DK1200597T3 (en)
ES (1) ES2264936T3 (en)
FR (1) FR2796081B1 (en)
PT (1) PT1200597E (en)
WO (1) WO2001004324A1 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2796081B1 (en) 1999-07-09 2003-09-26 Agronomique Inst Nat Rech PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A MICROORGANISM RECOMBINANT IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS
WO2004056963A2 (en) 2002-12-16 2004-07-08 E.I. Du Pont De Nemours And Company B12 dependent dehydratases with improved reaction kinetics
CN1935991B (en) * 2006-06-21 2010-05-12 南宁中诺生物工程有限责任公司 Clostridium perfringen glycerol dehydrase gene, and its 1,3-propylene glycol producing method
KR20090090319A (en) 2006-10-31 2009-08-25 메타볼릭 익스플로러 Method for biologically producing 1,3-propanediol from glycerol in high yield
WO2008052596A1 (en) * 2006-10-31 2008-05-08 Metabolic Explorer Process for the biological production of n-butanol with high yield
DE102007027006A1 (en) * 2007-06-08 2008-12-11 Evonik Degussa Gmbh Microbiological production of aldehydes, in particular of 3-hydroxypropionaldehyde
JP5774493B2 (en) 2008-12-31 2015-09-09 メタボリック エクスプローラー Diol production method
DE102009002811A1 (en) * 2009-05-05 2010-11-11 Evonik Degussa Gmbh Enzymatic process for the preparation of aldehydes
EP2248904A1 (en) * 2009-05-05 2010-11-10 Metabolic Explorer Continuous culture for 1,3-propanediol production using high glycerine concentration
WO2011022651A1 (en) 2009-08-21 2011-02-24 John Mcbride Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms
WO2011042434A1 (en) 2009-10-05 2011-04-14 Metabolic Explorer 1,3-propanediol production with immobilised cells reactors
KR101090362B1 (en) 2009-10-29 2011-12-07 한국생명공학연구원 New method for producing 3-hydroxypropionic acid from glycerol
KR101220499B1 (en) * 2010-05-25 2013-01-10 한국생명공학연구원 Method for Preparing 3-Hydroxypropionic Acid from Glycerol with High Yield
AR083799A1 (en) 2010-11-10 2013-03-20 Metabolic Explorer Sa MICROORGANISMS FOR THE PRODUCTION OF 1,3-PROPANODIOL USING CONCENTRATION OF HIGH GLYCERINE
EP2540834A1 (en) * 2011-06-29 2013-01-02 Metabolic Explorer Method for the preparation of 1,3-propanediol
US8673602B2 (en) * 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US9527953B2 (en) 2012-11-19 2016-12-27 Samsung Electronics Co., Ltd. Continuous preparation for polyester
US9501147B2 (en) * 2013-12-29 2016-11-22 Immersion Corporation Haptic device incorporating stretch characteristics
EP3328997B1 (en) 2015-07-27 2020-12-23 Institut National Des Sciences Appliquees New polypeptide having ferredoxin-nadp+ reductase activity, polynucleotide encoding the same and uses thereof
CA3053436A1 (en) 2017-02-20 2018-08-23 Metabolic Explorer Microbial consortium for 1,3-propanediol production using high glycerol concentration
EP3438270A1 (en) 2017-08-04 2019-02-06 Metabolic Explorer Microorganism and method for improved 1,3-propanediol production by fermentation on a culture medium with high glycerine content
AR114547A1 (en) 2018-08-10 2020-09-16 Metabolic Explorer Sa MICROORGANISMS WITH IMPROVED PRODUCTION OF 1,3-PROPANODIOL AND BUTYRIC ACID
WO2022073014A1 (en) * 2020-09-30 2022-04-07 Genomium, Inc. Fermentation process to produce bioacrolein and bioacrylic acid
CN112358986B (en) * 2020-11-09 2022-10-21 华南理工大学 Clostridium butyricum and application thereof in production of 1,3-propylene glycol through immobilized fermentation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
US5633362A (en) 1995-05-12 1997-05-27 E. I. Du Pont De Nemours And Company Production of 1,3-propanediol from glycerol by recombinant bacteria expressing recombinant diol dehydratase
KR100509310B1 (en) 1996-11-13 2005-08-22 이 아이 듀폰 디 네모아 앤드 캄파니 Method for the Production of 1,3-Propanediol by recombinant Organisms
FR2796081B1 (en) 1999-07-09 2003-09-26 Agronomique Inst Nat Rech PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A MICROORGANISM RECOMBINANT IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS

Also Published As

Publication number Publication date
ES2264936T3 (en) 2007-02-01
US7695943B2 (en) 2010-04-13
US20090029431A1 (en) 2009-01-29
DK1200597T3 (en) 2006-10-23
AU6296400A (en) 2001-01-30
US20100129892A1 (en) 2010-05-27
ATE330014T1 (en) 2006-07-15
US20030175916A1 (en) 2003-09-18
FR2796081B1 (en) 2003-09-26
CY1105334T1 (en) 2010-03-03
WO2001004324A1 (en) 2001-01-18
EP1200597A1 (en) 2002-05-02
PT1200597E (en) 2006-09-29
US7858355B2 (en) 2010-12-28
DE60028777T2 (en) 2007-05-31
FR2796081A1 (en) 2001-01-12
EP1200597B1 (en) 2006-06-14
US7267972B2 (en) 2007-09-11

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