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DE60011187D1 - Ligation of double-stranded DNAs in the presence of RecA - Google Patents

Ligation of double-stranded DNAs in the presence of RecA

Info

Publication number
DE60011187D1
DE60011187D1 DE60011187T DE60011187T DE60011187D1 DE 60011187 D1 DE60011187 D1 DE 60011187D1 DE 60011187 T DE60011187 T DE 60011187T DE 60011187 T DE60011187 T DE 60011187T DE 60011187 D1 DE60011187 D1 DE 60011187D1
Authority
DE
Germany
Prior art keywords
reca
ligation
double
stranded dnas
dnas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60011187T
Other languages
German (de)
Other versions
DE60011187T2 (en
Inventor
Yasushi Shigemori
Michio Oishi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aisin Corp
Original Assignee
Aisin Seiki Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aisin Seiki Co Ltd filed Critical Aisin Seiki Co Ltd
Application granted granted Critical
Publication of DE60011187D1 publication Critical patent/DE60011187D1/en
Publication of DE60011187T2 publication Critical patent/DE60011187T2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60011187T 1999-07-02 2000-06-30 Ligation of double-stranded DNAs in the presence of RecA Expired - Fee Related DE60011187T2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP18921199A JP4465741B2 (en) 1999-07-02 1999-07-02 Ligation at the end of a double-stranded DNA fragment
JP18921199 1999-07-02

Publications (2)

Publication Number Publication Date
DE60011187D1 true DE60011187D1 (en) 2004-07-08
DE60011187T2 DE60011187T2 (en) 2005-07-07

Family

ID=16237415

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60011187T Expired - Fee Related DE60011187T2 (en) 1999-07-02 2000-06-30 Ligation of double-stranded DNAs in the presence of RecA

Country Status (4)

Country Link
US (1) US7022501B1 (en)
EP (1) EP1065279B1 (en)
JP (1) JP4465741B2 (en)
DE (1) DE60011187T2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4616988B2 (en) 2000-12-20 2011-01-19 株式会社アイシン・コスモス研究所 DNA polymorphism detection method applying triple-stranded DNA formation technology
JP4220744B2 (en) * 2001-09-25 2009-02-04 株式会社アイシン・コスモス研究所 Processing libraries using ligation inhibition
JP2005523014A (en) * 2002-04-16 2005-08-04 プロメガ　コーポレイション Method for enhancing homologous recombination
CN108118085A (en) * 2016-11-29 2018-06-05 广州康昕瑞基因健康科技有限公司 Recombinase strand-displacement activity detection method and kit
BR112019028221A2 (en) * 2017-07-05 2020-07-07 OriCiro Genomics, Inc. DNA production method and DNA fragment junction kit
JPWO2023038145A1 (en) * 2021-09-13 2023-03-16
CN114908083B (en) * 2022-07-18 2023-05-05 中山大学附属第七医院(深圳) Method for in-vitro rapid synthesis of double-stranded circular DNA

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU658386B2 (en) * 1990-11-09 1995-04-13 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Method of targeting DNA
EP0842283A1 (en) * 1995-07-21 1998-05-20 THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES RecA-ASSISTED CLONING OF DNA
JP4265028B2 (en) 1999-04-14 2009-05-20 アイシン精機株式会社 Method for specific cleavage of double-stranded DNA and kit therefor

Also Published As

Publication number Publication date
US7022501B1 (en) 2006-04-04
JP2001017172A (en) 2001-01-23
EP1065279A1 (en) 2001-01-03
JP4465741B2 (en) 2010-05-19
DE60011187T2 (en) 2005-07-07
EP1065279B1 (en) 2004-06-02

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee