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DE50115604D1 - SYNTHETIC SPIDER SILK PROTEINS AND THEIR EXPRESSION IN TRANSGENIC PLANTS - Google Patents

SYNTHETIC SPIDER SILK PROTEINS AND THEIR EXPRESSION IN TRANSGENIC PLANTS

Info

Publication number
DE50115604D1
DE50115604D1 DE50115604T DE50115604T DE50115604D1 DE 50115604 D1 DE50115604 D1 DE 50115604D1 DE 50115604 T DE50115604 T DE 50115604T DE 50115604 T DE50115604 T DE 50115604T DE 50115604 D1 DE50115604 D1 DE 50115604D1
Authority
DE
Germany
Prior art keywords
encoding
protein
spider silk
plant
iii
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE50115604T
Other languages
German (de)
Inventor
Juergen Scheller
Udo Conrad
Frank Grosse
Karl-Heinz Guehrs
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LEIBNIZ INST fur PFLANZENGENE
Original Assignee
LEIBNIZ INST fur PFLANZENGENE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LEIBNIZ INST fur PFLANZENGENE filed Critical LEIBNIZ INST fur PFLANZENGENE
Priority to DE50115604T priority Critical patent/DE50115604D1/en
Priority claimed from PCT/EP2001/006586 external-priority patent/WO2001094393A2/en
Application granted granted Critical
Publication of DE50115604D1 publication Critical patent/DE50115604D1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

DNA sequence (I), encoding a synthetic spider silk protein (II), comprising modules, each comprising a group of sequentially arranged oligonucleotides (ON), each ON encoding a repeating unit of a spidroin protein (III), is new. DNA sequence (I), encoding a synthetic spider silk protein (II), comprising modules, each comprising a group of sequentially arranged oligonucleotides (ON), each ON encoding a repeating unit of a spidroin protein (III). (II) has at least 84, best 94,% homology with (III), especially the spidroin-1 protein of Nephila clavipes. Independent claims are also included for the following: (a) recombinant nucleic acid (Ia) comprising (I) and a ubiquitously active promoter; (b) vector containing (Ia); (c) microorganisms containing (Ia) or the vector of (b); (d) recombinant (II); (e) preparation of a plant, or plant cell, that produces (II); (f) transgenic plant cell containing (Ia) or the vector of (b), or produced by method (e); (g) transgenic plants containing the cells of (Ia), also their parts, harvested products or replicative material; (h) method for producing plant-derived or otherwise recombinant (II); and (i) plant-derived (II) produced by method (h).
DE50115604T 2000-06-09 2001-06-11 SYNTHETIC SPIDER SILK PROTEINS AND THEIR EXPRESSION IN TRANSGENIC PLANTS Expired - Lifetime DE50115604D1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE50115604T DE50115604D1 (en) 2000-06-09 2001-06-11 SYNTHETIC SPIDER SILK PROTEINS AND THEIR EXPRESSION IN TRANSGENIC PLANTS

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE10028212 2000-06-09
DE10053478 2000-10-24
DE10113781A DE10113781A1 (en) 2000-06-09 2001-03-21 New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins
PCT/EP2001/006586 WO2001094393A2 (en) 2000-06-09 2001-06-11 Synthetic spider silk proteins and the expression thereof in transgenic plants
DE50115604T DE50115604D1 (en) 2000-06-09 2001-06-11 SYNTHETIC SPIDER SILK PROTEINS AND THEIR EXPRESSION IN TRANSGENIC PLANTS

Publications (1)

Publication Number Publication Date
DE50115604D1 true DE50115604D1 (en) 2010-10-07

Family

ID=26006000

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10113781A Withdrawn DE10113781A1 (en) 2000-06-09 2001-03-21 New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins
DE50115604T Expired - Lifetime DE50115604D1 (en) 2000-06-09 2001-06-11 SYNTHETIC SPIDER SILK PROTEINS AND THEIR EXPRESSION IN TRANSGENIC PLANTS

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10113781A Withdrawn DE10113781A1 (en) 2000-06-09 2001-03-21 New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins

Country Status (2)

Country Link
AT (1) ATE478953T1 (en)
DE (2) DE10113781A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2399820A (en) * 2002-01-11 2004-09-29 Nexia Biotech Inc Recovery of biofilament proteins from biological fluids
US7060260B2 (en) 2003-02-20 2006-06-13 E.I. Du Pont De Nemours And Company Water-soluble silk proteins in compositions for skin care, hair care or hair coloring
AU2006333611B2 (en) 2005-12-30 2012-04-26 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins
CN116425848B (en) * 2023-04-11 2024-05-24 北京新诚中科技术有限公司 Recombinant chimeric spider silk protein, biological protein fiber, and preparation methods and applications thereof

Also Published As

Publication number Publication date
DE10113781A1 (en) 2001-12-13
ATE478953T1 (en) 2010-09-15

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