DE3686399D1 - Herstellung von thrombin-inhibitoren. - Google Patents

Herstellung von thrombin-inhibitoren.

Info

Publication number: DE3686399D1
Authority: DE; Germany
Prior art keywords: production; thrombin inhibitors; eukaryotic host; hybrid vectors; desulphatohirudin
Prior art date: 1985-12-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE8686117098T

Other languages

English (en)

Other versions

DE3686399T2 (de

Inventor

Walter Marki

Bernd Meyhack

Jutta Heim

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

UCP GEN-PHARMA AG ZUERICH CH

Original Assignee

Ciba Geigy AG

UCP Gen Pharma AG

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-12-12

Filing date

1986-12-09

Publication date

1992-09-17

1985-12-12 Priority claimed from GB858530631A external-priority patent/GB8530631D0/en

1986-12-09 Application filed by Ciba Geigy AG, UCP Gen Pharma AG filed Critical Ciba Geigy AG

1992-09-17 Application granted granted Critical

1992-09-17 Publication of DE3686399D1 publication Critical patent/DE3686399D1/de

1993-01-14 Publication of DE3686399T2 publication Critical patent/DE3686399T2/de

2006-12-10 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

238000004519 manufacturing process Methods 0.000 title abstract 2
229940122388 Thrombin inhibitor Drugs 0.000 title 1
239000003868 thrombin inhibitor Substances 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 2
239000013598 vector Substances 0.000 abstract 2
108010007267 Hirudins Proteins 0.000 abstract 1
102000007625 Hirudins Human genes 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
108010073652 desirudin Proteins 0.000 abstract 1
XYWBJDRHGNULKG-OUMQNGNKSA-N desirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 XYWBJDRHGNULKG-OUMQNGNKSA-N 0.000 abstract 1
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 abstract 1
229940006607 hirudin Drugs 0.000 abstract 1
238000000034 method Methods 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
230000028327 secretion Effects 0.000 abstract 1
238000011144 upstream manufacturing Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Mycology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Diabetes (AREA)
Tropical Medicine & Parasitology (AREA)
Pharmacology & Pharmacy (AREA)
General Chemical & Material Sciences (AREA)
Animal Behavior & Ethology (AREA)
Hematology (AREA)
Public Health (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Veterinary Medicine (AREA)
Chemical Kinetics & Catalysis (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

DE8686117098T 1985-12-12 1986-12-09 Herstellung von thrombin-inhibitoren. Expired - Lifetime DE3686399T2 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB858530631A GB8530631D0 (en)	1985-12-12	1985-12-12	Thrombin inhibitors
GB868613088A GB8613088D0 (en)	1985-12-12	1986-05-29	Thrombin inhibitors

Publications (2)

Publication Number	Publication Date
DE3686399D1 true DE3686399D1 (de)	1992-09-17
DE3686399T2 DE3686399T2 (de)	1993-01-14

Family

ID=26290108

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE8686117098T Expired - Lifetime DE3686399T2 (de)	1985-12-12	1986-12-09	Herstellung von thrombin-inhibitoren.

Country Status (17)

Country	Link
EP (1)	EP0225633B1 (de)
JP (2)	JPH0650990B2 (de)
AT (1)	ATE79403T1 (de)
AU (1)	AU615413B2 (de)
CA (1)	CA1341496C (de)
DE (1)	DE3686399T2 (de)
DK (1)	DK175492B1 (de)
ES (1)	ES2051688T3 (de)
FI (1)	FI94773C (de)
GR (1)	GR3006248T3 (de)
HU (1)	HU209146B (de)
IE (1)	IE57859B1 (de)
IL (1)	IL80932A (de)
MY (1)	MY101203A (de)
NO (1)	NO178035C (de)
PH (1)	PH31277A (de)
PT (1)	PT83898B (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3583361D1 (de) *	1984-06-14	1991-08-08	Ucp Gen Pharma Ag	Verfahren zur herstellung von thrombin-inhibitoren.
MC1834A1 (fr) *	1986-07-10	1988-06-03	Transgene Sa	Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
GB8620926D0 (en) *	1986-08-29	1986-10-08	Delta Biotechnology Ltd	Yeast promoter
US6005071A (en) *	1987-01-23	1999-12-21	Merrell Pharmaceuticals Inc.	Anticoagulant peptides
US5789540A (en) *	1987-01-23	1998-08-04	Merrell Pharmaceuticals Inc.	Anticoagulant peptides
CA1341032C (en) *	1987-01-23	2000-06-20	John L. Krstenansky	Anticoagulant peptides
US5256559A (en) *	1988-03-04	1993-10-26	Biogen, Inc.	Methods and compositions for inhibiting platelet aggregation
GB8809860D0 (en) *	1988-04-26	1988-06-02	Ciba Geigy Ag	Process for production of polypeptides
GB8907110D0 (en) *	1988-05-04	1989-05-10	Ciba Geigy Ag	Improvements in the production of polypeptides
US5268296A (en) *	1988-06-11	1993-12-07	Ciba-Geigy Corporation	DNA vector and recombinant host cell for production of hirullin P6 and P18
US5167960A (en) *	1988-08-03	1992-12-01	New England Deaconess Hospital Corporation	Hirudin-coated biocompatible substance
US5112615A (en) *	1988-08-03	1992-05-12	New England Deaconess Hospital Corporation	Soluble hirudin conjugates
GB8826428D0 (en) *	1988-11-11	1988-12-14	Biopharm Ltd	Antithrombin
FR2646437B1 (fr) *	1989-04-28	1991-08-30	Transgene Sa	Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
US5179196A (en) *	1989-05-04	1993-01-12	Sri International	Purification of proteins employing ctap-iii fusions
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
US6514730B1 (en)	1991-03-21	2003-02-04	Consortium für elektrochemische Industrie GmbH	Secretion of hirudin derivatives
SG49859A1 (en) *	1992-09-04	2002-03-19	Ucp Gen Pharma Ag	Process for the production of protease inhibitors
DE69616276T2 (de)	1995-02-09	2002-12-12	Aventis Pharma Deutschland Gmbh	Verfahren zur produktion von proteinen
US6468098B1 (en)	1999-08-17	2002-10-22	Formfactor, Inc.	Electrical contactor especially wafer level contactor using fluid pressure
US7795205B2 (en)	2004-04-12	2010-09-14	Canyon Pharmaceuticals, Inc.	Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
JP7284955B2 (ja) *	2020-07-20	2023-06-01	フォーデイズ株式会社	神経幹細胞の増殖促進剤及びそれを用いた神経幹細胞の増殖促進方法
WO2025262265A1 (en)	2024-06-21	2025-12-26	Etablissement Francais Du Sang	Chimeric molecules comprising an anti-fibrin antibody and an antithrombotic molecule

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1984004330A1 (en) *	1983-04-22	1984-11-08	Amgen	Secretion of exogenous polypeptides from yeast
EP0656418B1 (de) *	1983-10-03	2002-12-04	Chiron Corporation	Klonieren von Superoxiddismutase und Expression in Mikroorganismen
FI86746C (fi) *	1983-11-21	1992-10-12	Ciba Geigy Ag	Foerfarande foer framstaellning av maensklig vaevnads-plasminogenaktivator (tpa) med hjaelp av saccharomyces cerevisiae -jaest och i foerfarandet anvaenda hybridvektorer
DE3342139A1 (de) *	1983-11-22	1985-05-30	Ciba-Geigy Ag, Basel	Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
CA1341414C (fr) *	1984-03-27	2002-12-31	Paul Tolstoshev	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3583361D1 (de) *	1984-06-14	1991-08-08	Ucp Gen Pharma Ag	Verfahren zur herstellung von thrombin-inhibitoren.
FR2568891B1 (fr) *	1984-08-09	1988-05-27	Transgene Sa	Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
GB8521496D0 (en) *	1985-08-29	1985-10-02	Ciba Geigy Ag	Repressible yeast promoters
MC1834A1 (fr) *	1986-07-10	1988-06-03	Transgene Sa	Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation

1986
- 1986-12-04 MY MYPI86000173A patent/MY101203A/en unknown
- 1986-12-08 FI FI865003A patent/FI94773C/fi not_active IP Right Cessation
- 1986-12-09 EP EP86117098A patent/EP0225633B1/de not_active Expired - Lifetime
- 1986-12-09 ES ES86117098T patent/ES2051688T3/es not_active Expired - Lifetime
- 1986-12-09 DE DE8686117098T patent/DE3686399T2/de not_active Expired - Lifetime
- 1986-12-09 AT AT86117098T patent/ATE79403T1/de not_active IP Right Cessation
- 1986-12-10 PT PT83898A patent/PT83898B/pt unknown
- 1986-12-10 CA CA000524903A patent/CA1341496C/en not_active Expired - Fee Related
- 1986-12-10 IL IL80932A patent/IL80932A/en not_active IP Right Cessation
- 1986-12-11 HU HU865177A patent/HU209146B/hu unknown
- 1986-12-11 DK DK198605952A patent/DK175492B1/da not_active IP Right Cessation
- 1986-12-11 NO NO865007A patent/NO178035C/no not_active IP Right Cessation
- 1986-12-11 AU AU66504/86A patent/AU615413B2/en not_active Expired
- 1986-12-11 IE IE3244/86A patent/IE57859B1/en not_active IP Right Cessation
- 1986-12-11 JP JP61293558A patent/JPH0650990B2/ja not_active Expired - Lifetime
1988
- 1988-04-07 PH PH36763A patent/PH31277A/en unknown
1992
- 1992-11-13 GR GR920401771T patent/GR3006248T3/el unknown
1993
- 1993-11-12 JP JP5282983A patent/JPH0787791B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
MY101203A (en)	1991-08-17
DE3686399T2 (de)	1993-01-14
NO865007L (no)	1987-06-15
AU6650486A (en)	1987-06-18
JPS62208296A (ja)	1987-09-12
EP0225633A2 (de)	1987-06-16
DK595286D0 (da)	1986-12-11
FI865003A0 (fi)	1986-12-08
EP0225633A3 (en)	1988-11-30
IE57859B1 (en)	1993-04-21
FI865003L (fi)	1987-06-13
ES2051688T3 (es)	1994-07-01
EP0225633B1 (de)	1992-08-12
PH31277A (en)	1998-07-06
ATE79403T1 (de)	1992-08-15
GR3006248T3 (de)	1993-06-21
NO865007D0 (no)	1986-12-11
IE863244L (en)	1987-06-12
FI94773B (fi)	1995-07-14
DK595286A (da)	1987-06-13
CA1341496C (en)	2006-02-14
IL80932A0 (en)	1987-03-31
NO178035B (no)	1995-10-02
HUT42523A (en)	1987-07-28
IL80932A (en)	1998-02-22
NO178035C (no)	1996-01-10
HU209146B (en)	1994-03-28
JPH0787791B2 (ja)	1995-09-27
JPH0650990B2 (ja)	1994-07-06
PT83898A (en)	1987-01-01
PT83898B (pt)	1989-06-30
AU615413B2 (en)	1991-10-03
FI94773C (fi)	1995-10-25
DK175492B1 (da)	2004-11-08
JPH06197766A (ja)	1994-07-19

Publication	Publication Date	Title
DE3686399D1 (de)	1992-09-17	Herstellung von thrombin-inhibitoren.
ATE158816T1 (de)	1997-10-15	Expression von pace in wirtszellen und verfahren zu dessen verwendung
ATE91714T1 (de)	1993-08-15	Transformation von trichoderma.
PT88479A (pt)	1989-07-31	Metodo para cultura de celulas recombinantes
ATE144281T1 (de)	1996-11-15	Hefezellen der gattung-schwanniomyces
ATE90727T1 (de)	1993-07-15	Dns-konstruktionen, enthaltend eine kluyveromyces-alpha-factor-leadersequenz zur leitung der sekretion von heterologen polypeptiden.
FI831818L (fi)	1983-11-29	Mikrobiologiskt framstaellda - och -interferoner, dessa interferoner kodande dna-sekvenser, dennagenetiska information innehaollande mikroorganismer och deras framstaellningsfoerfarande
FI90667C (fi)	1994-03-10	Menetelmä valmistaa uutta ihmisen tyypin I interferoniproteiinia
ES2055149T3 (es)	1994-08-16	Derivado de hirudina.
ATE63335T1 (de)	1991-05-15	Herstellung von rekombinantem protein.
DE59108128D1 (de)	1996-10-10	Verfahren zur Herstellung von Fremdproteinen in Streptomyceten
DK509886D0 (da)	1986-10-24	Udtrykkelsesforoegende dna-sekvenser
ATE102994T1 (de)	1994-04-15	Verfahren zur herstellung von proteinen.
DE3689175D1 (de)	1993-11-25	Rekombinante Wirte, deren Herstellung und Verwendung.

Legal Events

Date	Code	Title	Description
1993-09-09	8364	No opposition during term of opposition
1997-09-11	8327	Change in the person/name/address of the patent owner	Owner name: NOVARTIS AG, BASEL, CH UCP GEN-PHARMA AG, KIRCHBER
2005-01-13	8327	Change in the person/name/address of the patent owner	Owner name: UCP GEN-PHARMA AG, ZUERICH, CH
2005-01-13	8328	Change in the person/name/address of the agent	Representative=s name: REITSTOETTER, KINZEBACH & PARTNER (GBR), 81679 MUENCHEN